The infestation by nematodes of the genus Haemonchus is a very relevant problem in small ruminant livestock production in tropical countries. This disease causes weight loss, immunosuppression, and gastroenteritis-associated malabsorption syndrome that affect the animals' welfare and normal development. Else, causing significant economic losses in the sheep production system due to both the effect on product yield and reproduction (1-5).
Gastrointestinal nematodes of the genus Haemonchus are hematophagous parasites and are the primary pathogen agent in the ruminants abomasum (6). Adult parasites of the H. contortus, H. placei, and H. similis the species can consume up to 0.05 mL of blood/day from their host, having a high clinical impact (7). The parasite sexual reproduction takes place in a natural host life cycle. Females are prolific, laying up to 10,000 eggs/day (pre-patent period of 3-5 weeks), which leads to a large number of infective and pre-infective forms of the parasite in the grasslands (eggs and L1, L2, and L3) (8). According to Stromberg and Gasbarre (9), the Haemonchus sp. larvae with infective capacity (L3) migrate from feces to forage approximately seven days post-hatch at 23 °C and 65% RH (8, 9). Later, Silva et al. (10) demonstrated that the L3 vertical migration on the forage is critical in the parasite transmission, allowing its ingestion by the grazing host. This migratory behavior is influenced by climatic conditions such as temperature, relative humidity, sunlight, and rainfall (3, 11-13).
H. contortus and H. placei have been reported in Colombian herds since 1983 (14). Researches on the parasite ecology has been conducted in different agroclimatic zones (15-19). In a 4-year study using weaned calves, Parra and Uribe (20) found a high level of pasture infestation with Haemonchus sp. larvae associated with periods of high rainfall (April to June, and August to September) in the piedmont (foothills) of the eastern Colombian plains.
Worldwide, this parasitosis control has been implemented since the 19th century, mainly using regular pharmaceutical compounds. Nevertheless, due to these products’ unsupervised dosage regimes, the parasitic organisms have developed resistance (1, 21-23). Consequently, FAO recommends developing sustainable pest control strategies in animal production systems (e.g., against gastrointestinal parasites); sustainable from the environmental perspective and long-time effective from the animal health perspective (24). Thus, based on the large numbers of ethnopharmacological reports, the research on medicinal plant extracts for parasite control has become a widely distributed scientific practice that had yield relevant findings for integrated pest management practice (25-28). Moreover, the use of plant extracts can have less impact on the environment due to their short biodegradation time in the soil compared to traditional pharmaceutical compounds (29).
Various types of traditional plants for medical use have been studied to obtain extracts that present potential antiparasitic activity. Hence, the anthelmintic activity of M. charantia has been registered in in-vitro and in-vivo trials and by phytochemical analyses in other latitudes (30-32). M. charantia is known as "the bitter melon" and belongs to the Cucurbitaceae family (33). The plant grows in tropical areas of Asia, Amazon, East Africa, and the Caribbean. It is cultivated throughout the world as a food source and for medicinal purposes (34).
M. charantia has been used traditionally as a medicinal plant in different countries such as Brazil, China, Cuba, Ghana, Haiti, India, México, Malaysia, New Zealand, Nicaragua, Panamá Perú, and Colombia (35-37). Some reports have already described the exploration of the potential effects of M. charantia extracts for the control of H. contortus in small ruminants (38-40). In this regard, the anthelmintic capacity of M. charantia has been attributed to different secondary metabolites such as the triterpene compound called cucurbitacin B, the non-protein amino acid called cucurbitin (3-amino-pyrrolidine-3-carboxylic acid), many saponins, and various sterols (41, 42). Other compounds such as cucurmosin (ribosome-inactivating protein) found in stem and leaves have also been studied (43).
The objective of this study was to evaluate the in-vitro effect of the methanolic extracts of M. charantia on the hatching process of Haemonchus sp. nematode as a potential therapeutic alternative for integrated parasite management programs in sheep production systems of the piedmont region in Meta, Colombia.
10 Kg of M. charantia leaves were collected in the Barcelona farm of Universidad de Los Llanos (Villavicencio, Meta, Colombia) during February (dry season). A sample of the leaf, stem, and fruit-flowering was sent to the Colombian National Herbarium to perform a taxonomic classification. The methanolic extract was made from M. charantia leaves, dried in a recirculating air oven at 40 °C for 72 hours. Then, the dry material was pulverized, obtaining a homogeneous sifting. The material was percolated continually until exhaustion with methanol 98% (Merck®, Germany). The percolated liquid was filtered and concentrated at 40°C using a Roto-evaporator (IKA®, Brazil). The resulting extract was reconstituted in 0.5% dimethyl sulfoxide solution (DMSO) (Merck®, Germany) to 10, 20, 40, 80, and 160 mg/mL concentrations for the in-vitro tests, following the methodology proposed by Domingues et al. (28).
Following the Michael et al. (45) methodology, eggs of Haemonchus sp. were obtained from 20g of feces taken from the rectal ampulla of a monoinfected sheep. Its collection and concentration were performed using different sieves (1 mm, 106 μm, 53 μm, and 25 μm mesh) according to the method published by Bizimenyera et al. (46) based on the original method reported by Coles et al. (47). The effect of the extract in eggs was evaluated according to the guidelines proposed by the WAAVP (World Association for the Advancement of Veterinary Parasitology) and described by Powers et al. (48). Briefly, approximately 100 eggs were exposed (250 μL final volume) to the five treatment levels of the methanolic extract of M. charantia, to 25 µg/mL of Albendazole (Sigma®, Germany; positive control), and 0.5% DMSO (negative control) (Table 1). Each test was replicated five times; all tests were performed 24 hours at 27°C and RH>80%. After that, a drop of Lugol's Iodine solution (PipingRock®, U.S.A.) was added to stop the hatching process. Larvae (L1) and eggs were counted using an inverted microscope (Leica®, Germany). The hatching inhibition percentage (HI %) was determined for each level of treatment with the following formula: HI % = 100 (P test/P total), where "P test" is the number of eggs and "P total" is the number of eggs plus the count of L1 individuals. This research was approved by the General Director of Research of the Universidad de Los Llanos through the project "Alternativa terapéutica para el control de la haemoncosis con base en el extracto metanólico de Momordica charantia en sistemas de producción bovina del piedemonte del departamento del Meta" according to announcement 01-P-2013, which guarantees the humane treatment of the experimental animals used.
The preliminary phytochemical analysis of M. charantia leaves was conducted following the colorimetric methods and thin-layer chromatography methodology proposed by Sanabria (44). These methods permit the detection of alkaloids, steroids or triterpenoids, flavonoids, naphthokines or anthraquinones, tannins, lactones, coumarins, and other cardiotonics.
The hatching inhibition values obtained are presented as hatching inhibition percentages and were compared to the negative control group (DMSO 0.5%) using the Chi-square test for comparing two proportions distributions (p<0,05). The LC50 and LC90 were estimated from egg hatching inhibition results through the Probit regression model analysis (estimate plus 95% confidence interval). The data were organized and analyzed with OpenStat 4.0, version 7.0 statistical program.
The methanolic extracts of M. charantia showed satisfactory hatching inhibition results on Haemonchus sp eggs (Table 1). Concentrations from 1 to 8% (10 to 80 mg/mL) showed significant differences with the DMSO 0.5% negative control group (χ2 = 0.024, p-value < 0.05). Likewise, inhibitory action to the hatching of Haemonchus sp. eggs in the concentration of 16% (160 mg/mL) of the plant extract was similar to the Albendazole 25µg/mL control group. We also found significant differences in hatching inhibition percentages (p-value < 0.001) to DMSO 0.5%. For this study, according to the Probit regression results, the LC50 estimated was 52.18 mg/mL (95%CI: 37.87 - 63.22), and the estimated LC90 was 201.45 mg/mL (95%CI: 186.01 - 221.89).
| Extract concentration - controls | % Hatching inhibition |
|---|---|
| 10 mg/mL | 38.3% A |
| 20 mg/mL | 24.2% A |
| 40 mg/mL | 34.5% A |
| 80 mg/mL | 50.4%A |
| 160 mg/mL | 84.6% B |
| Albendazole 25 µg/mL | 100%B |
| DMSO 5 mg/mL | 0% |
Table 2, presents the characterization of secondary metabolites groups in the methanolic extract of M. charantia, obtained by qualitative techniques of colorimetry and thin-layer chromatography. These techniques allowed us to characterize alkaloids, triterpenes, and anthracenic glucosides.
M. charantia has been used in traditional medicine (30-32, 34-37). The phytochemical assets of its extracts have been appraised due to antibacterial (49, 50), anticancer (51, 52), antidiabetic (53, 54), antidepressant (55), antifungal (56, 57), antiviral (58), and anthelmintic (30-32, 59-62) properties, among others. Likewise, nutritional analyses of M. charantia indicate that this plant is rich in fiber, calcium, potassium, iron, and vitamins A and C (63). The pulp around the ripe fruit seeds is a good source of carotenoid lycopene (64). Besides, M. charantia is an important source of phenolic compounds with high antioxidant and antimutagenic properties (65).
The plant extracts action mechanisms can affect different stages of the Haemonchus sp. life cycle, including the free-living phase (pre-parasitic) and either the L3 infective and parasitic phases. The antihelmintic effect of cucurbits is related to the presence of hydrolases in M. charantia capable of binding to the parasite's cuticle, activating a proteinase enzymatic complex that promotes the digestion of the parasite's cuticle, finally causing the death of the parasite (78-80). Regarding the results of our study, the catalytic activity of the anthracenic glycosides found in M. charantia (Table 2) could be responsible for the degradation of the Haemonchus sp. eggshell membrane. It may play a central role in the antiparasitic activity (81). However, further research is necessary to elaborate elucidation of the chemical processes involved.
Beloin et al. (82) worked with different extracts of M. charantia leaves and found a direct anthelmintic effect of momordicins (saponins) and triterpenes on Caenorhabditis elegans (nematode) using a 500 mg/mL lethal concentration. Similar results were reported in a study by Ling et al. (83). They found momordicins in an ethanolic extract of M. charantia, and demonstrated the adverse effects on the feeding processes of the L2 and L3 stages of Plutella xylostella (a pest of ornamental crops). They also reported lethal larval concentrations of momordicin II of 76, 69, and 116.24 mg/mL, respectively. Other effects reported were inhibition of weight gain and reduced larval survival.
Studies by Islam et al. (84) used aqueous methanolic and ethanolic extracts of M. charantia leaves, showing effects on the hatching of Ascaridia galli eggs. Using a concentration of 4% of the extract, they reported 70% and 67% eggs development adverse effects with the aqueous extract and 65% with the methanolic extract. These results indicated that a higher concentration was needed and support the concentration used in the present study (16%) to achieve similar results to the positive control (Table 1).
Tjokropranoto and Nathania (59) studied the effect of M. charantia in adult nematodes. From an in-vivo experiment in pigs, they reported that after treatments with an ethanolic extract of M. charantia leaves at concentrations of 10%, 20%, and 40%, the average percentage of Ascaris suum that were paralyzed or killed was 75%, 83%, and 88% respectively. Likewise, Chastity et al. (60) studied an ethanolic extract of M. charantia leaves to estimate the mortality rate on Ascaris suum. They found that the adult nematodes mortality time was 10 hours, with the 80% extract concentration compared to the 4 hours required using pyrantel pamoate (positive control). Besides, Shahadat et al. (61) y Alam et al. (62) studied the effect of aqueous extracts of M. charantia on Ascardia galli affecting broilers. They found very high mortality rates (75% and 95%, respectively) using concentrations up to 100 mg/mL. This high concentration was chosen to induce short-term mortalities. From the studies described, it is inferred that more studies are needed on the effect of M. charantia extract in adult and larval stages of Haemonchus sp.
We confirmed the presence of terpenes, alkaloids, and anthraquinone glycosides, which are important secondary metabolites attributed to M. charantia with antihelmintic potential (Table 2). These compounds have been reported for this purpose by Poolperm and Jiraungkoorskul (30), Morton (43), and Bauri et al. (66). These secondary metabolites can also be found in other medicinal plants with anthelmintic activity: terpenes (67-72), alkaloids (73-75), and glycosides (67, 70, 73, 76). However, flavonoids, other anthelmintic metabolites described in M. charantia were not detected by our techniques (66, 77).
The scientific inferences exposed in this study, similar to others in the detection of secondary metabolites groups of and describing their anthelmintic effects, confirm the potential of M. charantia extracts as beneficial compounds to be part of the integrated parasite control programs in livestock production systems in the tropics.
In the in-vitro test, the methanolic extract of M. charantia effectively inhibited the hatching of Haemonchus sp. The presence of secondary metabolites like alkaloids, triterpenes, and anthracene glucosides was detected in the methanolic extract of M. charantia (under the given collection period, location, and conditions).
It is necessary to develop a process of bio-guided fractionation of the methanolic extract of M. charantia in order further to purify the relevant compounds and evaluate its biological activity on different life stages of H. contortus; this being a plant species widely adapted to the conditions of the piedmont (foothills) of Meta, Colombia.
Kelly V. Martínez for her support and collaboration in animal care and welfare assurance and her experimental phase contributions.
1. Drudge J, Szanto J, Wyant Z, Elam G. Field studies on parasite control in sheep: comparison of thiabendazole, ruelene, and phenothiazine. Am. J. Vet. Res [Internet]. 1964;25:1512-1518. Available from: https://www.scienceopen.com/document?vid=1571be24-967d-4592-b8fd-c9ae1d5196ec
J Drudge J Szanto Z Wyant G Elam Field studies on parasite control in sheep: comparison of thiabendazole, ruelene, and phenothiazineAm. J. Vet. Res19642515121518https://www.scienceopen.com/document?vid=1571be24-967d-4592-b8fd-c9ae1d5196ec
2. Grisi L. O problema de parasitismo interno dos bovinos nos tropicos. Seminario internacional: Manejo y control de ecto y endoparásitos en ganado bovino, Convenio ICA-GTZ-Unisalle, Cartagena de Indias; 1993.
L Grisi O problema de parasitismo interno dos bovinos nos tropicosSeminario internacional: Manejo y control de ecto y endoparásitos en ganado bovinoConvenio ICA-GTZ-UnisalleCartagena de Indias1993
3. Agyei A. Seasonal changes in the level of infective strongylate nematodes larvae on pasture in the coastal savanna regions of Ghana. Vet. Parasitol. 1997;70:175-182. DOI: https://doi.org/10.1016/S0304-4017(96)01101-6
A Agyei Seasonal changes in the level of infective strongylate nematodes larvae on pasture in the coastal savanna regions of GhanaVet. Parasitol.199770175182https://doi.org/10.1016/S0304-4017(96)01101-6
5. Charlier J, Höglund J, Dorny P, von Samson-Himmelstjerna G, Vercruysse, J. Gastrointestinal nematode infections in adult dairy cattle: impact on production, diagnosis and control. Vet. Parasitol. 2009;164:70-79. DOI: https://doi.org/10.1016/j.vetpar.2009.04.012
Höglund J Charlier J von Samson-Himmelstjerna G Dorny P J Vercruysse Gastrointestinal nematode infections in adult dairy cattle: impact on production, diagnosis and controlVet. Parasitol.20091647079https://doi.org/10.1016/j.vetpar.2009.04.012
6. O'Connor L, Walkden S, Kahn L. Ecology of the free-living stages of major trichostrongylid parasites of sheep. Vet. Parasitol. 2006;142:1-15. DOI: https://doi.org/10.1016/j.vetpar.2006.08.035
L O'Connor S Walkden L Kahn Ecology of the free-living stages of major trichostrongylid parasites of sheepVet. Parasitol.2006142115https://doi.org/10.1016/j.vetpar.2006.08.035
8. Prichard R. Genetic variability following selection of Haemonchus contortus with anthelmintics. Trends Parasitol. 2001;17:445-453. DOI: https://doi.org/10.1016/s1471-4922(01)01983-3
R Prichard Genetic variability following selection of Haemonchus contortus with anthelminticsTrends Parasitol.200117445453https://doi.org/10.1016/s1471-4922(01)01983-3
9. Stromberg B, Gasbarre L. Gastrointestinal Nematode Control Programs with an Emphasis on Cattle. Vet Clin Food Anim. 2006;22:543-565. DOI: https://doi.org/10.1016/j.cvfa.2006.08.003
B Stromberg L Gasbarre Gastrointestinal Nematode Control Programs with an Emphasis on CattleVet Clin Food Anim.200622543565https://doi.org/10.1016/j.cvfa.2006.08.003
10. Silva B, Amarante M, Kadri S, Carrijo-Mauad J, Amarante A. Vertical migration of Haemonchus contortus third stage larvae on Brachiaria decumbens grass. Vet. Parasitol. 2008;158:85-92. DOI: https://doi.org/10.1016/j.vetpar.2008.08.009
B Silva M Amarante S Kadri J Carrijo-Mauad A Amarante Vertical migration of Haemonchus contortus third stage larvae on Brachiaria decumbens grassVet. Parasitol.20081588592https://doi.org/10.1016/j.vetpar.2008.08.009
11. Callinan A, Westcott J. Vertical distribution of trichostrongylid larvae on herbage and in soil. Int. J. Parasitol. 1986;16:241-244. DOI: https://doi.org/10.1016/0020-7519(86)90050-0
A Callinan J Westcott Vertical distribution of trichostrongylid larvae on herbage and in soilInt. J. Parasitol.198616241244https://doi.org/10.1016/0020-7519(86)90050-0
12. Silangwa S, Todd A. Vertical migration of Trichostrongylid larvae on grasses. J. Parasitol. 1964;50:278-285. DOI: https://doi.org/10.2307/3276286
S Silangwa A Todd Vertical migration of Trichostrongylid larvae on grassesJ. Parasitol.196450278285https://doi.org/10.2307/3276286
13. Hoberg E, Lichtenfels J, Gibbons L. Phylogeny for species of Haemonchus (Nematoda: Trichostrongyloidea): considerations of their evolutionary history and global biogeography among Camelidae and Pecora (Artiodactyla). J. Parasitol. 2004;90:1085-1102. DOI: https://doi.org/10.1645/ge-3309
E Hoberg J Lichtenfels L Gibbons Phylogeny for species of Haemonchus (Nematoda: Trichostrongyloidea): considerations of their evolutionary history and global biogeography among Camelidae and Pecora (Artiodactyla)J. Parasitol.20049010851102https://doi.org/10.1645/ge-3309
14. Rivera B, Parra D, García O, Aycardi E. Gastro-intestinal parasites in calves in Colombia. Tropical Animal Health and Production [Internet]. 1983;15:107-114. Available from: https://link.springer.com/article/10.1007/BF02239806
B Rivera D Parra O García E Aycardi Gastro-intestinal parasites in calves in ColombiaTropical Animal Health and Production198315107114https://link.springer.com/article/10.1007/BF02239806
15. Pinilla J, Florez P, Sierra M, Morales E, Sierra R, Vásquez M, et al. Prevalencia del parasitismo gastrointestinal en bovinos del Departamento del Cesar, Colombia. Rev. Inv. Perú. 2018;29:278-287. DOI: http://dx.doi.org/10.15381/rivep.v29i1.14202
J Pinilla P Florez M Sierra E Morales R Sierra M Vásquez Prevalencia del parasitismo gastrointestinal en bovinos del Departamento del Cesar, ColombiaRev. Inv. Perú.201829278287http://dx.doi.org/10.15381/rivep.v29i1.14202
16. Tullner F, Roqueme L, Otte J. Investigaciones sobre la ocurrencia, epidemiología, e importancia económica de los helmintos en terneros en el departamento de Córdoba, Colombia, ICA-GTZ, Informe Técnico N° 10, Bogotá; 1993; 1-58p
F Tullner L Roqueme J Otte Investigaciones sobre la ocurrencia, epidemiología, e importancia económica de los helmintos en terneros en el departamento de Córdoba, ColombiaICA-GTZInforme Técnico N° 10Bogotá1993158
17. Marquéz D, Jaramillo F, Romero A. Dinámica del parasitismo gastrointestinal en bovinos del hato de Tibaitata, Colombia. Revista de medicina Veterinaria y Zootecnia. Universidad Nacional de Colombia. 2000;47:49-56.
D Marquéz F Jaramillo A Romero Dinámica del parasitismo gastrointestinal en bovinos del hato de Tibaitata, ColombiaRevista de medicina Veterinaria y ZootecniaUniversidad Nacional de Colombia2000474956
18. Herrera L, Ríos L, Zapata R. Frecuencia de la infección por nemátodos gastrointestinales en ovinos y caprinos de cinco municipios de Antioquia. Rev. MVZ Córdoba. 2013;18:3852-2860. DOI: https://doi.org/10.21897/rmvz.157
L Herrera L Ríos R Zapata Frecuencia de la infección por nemátodos gastrointestinales en ovinos y caprinos de cinco municipios de AntioquiaRev. MVZ Córdoba.20131838522860https://doi.org/10.21897/rmvz.157
19. Marquéz D, García F, Jiménez G, Garzón C, Alarcón R, Basto G, et al. Diseño y estrategias para el control de ecto y endoparásitos del ganado en trópicos medio, bajo y de altura, de Cundinamarca y Boyacá. Informe Técnico Final Pronatta. Bogotá D.C. 2003.
D Marquéz F García G Jiménez C Garzón R Alarcón G Basto Diseño y estrategias para el control de ecto y endoparásitos del ganado en trópicos medio, bajo y de altura, de Cundinamarca y Boyacá. Informe Técnico Final PronattaBogotá D.C.2003
21. Lichtenfels J, Pilitt P, Hoberg E. New morphological characters for identifying individual specimens of Haemonchus spp. (Nematoda: Trichostrongyloidea) and a key to species in ruminants of North America. J. Parasitol. 1994;80:107-119. DOI: https://doi.org/10.2307/3283353
J Lichtenfels P Pilitt E Hoberg New morphological characters for identifying individual specimens of Haemonchus spp. (Nematoda: Trichostrongyloidea) and a key to species in ruminants of North AmericaJ. Parasitol.199480107119 https://doi.org/10.2307/3283353
22. Cruz D, Rocha L, Arruda S, Palieraqui J, Cordeiro R, Santos Junior E, Molento M, et al. Anthelmintic efficacy and management practices in sheep farms from the state of Rio de Janeiro, Brazil. Vet. Parasitol. 2010;170:340-343. DOI: https://doi.org/10.1016/j.vetpar.2010.02.030
D Cruz L Rocha S Arruda J Palieraqui R Cordeiro E Santos Junior M Molento Anthelmintic efficacy and management practices in sheep farms from the state of Rio de Janeiro, BrazilVet. Parasitol.2010170340343https://doi.org/10.1016/j.vetpar.2010.02.030
23. Brasil B, Nunes R, Bastianetto E, Drummond M, Carvalho D, Leite R, et al. Genetic diversity patterns of Haemonchus placei and Haemonchus contortus populations isolated from domestic ruminants in Brazil. International Journal for Parasitology. 2012;42:469-479. DOI: https://doi.org/10.1016/j.ijpara.2012.03.003
B Brasil R Nunes E Bastianetto M Drummond D Carvalho R Leite Genetic diversity patterns of Haemonchus placei and Haemonchus contortus populations isolated from domestic ruminants in BrazilInternational Journal for Parasitology201242469479https://doi.org/10.1016/j.ijpara.2012.03.003
24. Food and Agriculture Organization of the United Nations. Resistance Management and Integrated Parasite Control in Ruminants: Guidelines, 1 first ed, FAO Animal Production and Health Division. Agricultural Dept, Rome; 2004.
Food and Agriculture Organization of the United Nations Resistance Management and Integrated Parasite Control in Ruminants: Guidelines1FAO Animal Production and Health Division. Agricultural DeptRome2004
25. Rojas D, López J, Tejada I, Vázquez V, Shimada A, Sánchez D, et al. Impact of condensed tannins from tropical forages on Haemonchus contortus burdens in Mongolian gerbils (Meriones unguiculatus) and Pelibuey lambs. Anim. Feed Sci. Technol. 2006;128:218-228. DOI: https://doi.org/10.1016/j.anifeedsci.2005.10.008
D Rojas J López I Tejada V Vázquez A Shimada D Sánchez Impact of condensed tannins from tropical forages on Haemonchus contortus burdens in Mongolian gerbils (Meriones unguiculatus) and Pelibuey lambsAnim. Feed Sci. Technol.2006128218228https://doi.org/10.1016/j.anifeedsci.2005.10.008
26. Alonso M, Torres J, Sandoval C, Hoste H, Aguilar A. In vitro larval migration and kinetics of exsheathment of Haemonchus contortus larvae exposed to four tropical tanniniferous plant extracts. Vet. Parasitol. 2008;153:313-319. DOI: https://doi.org/10.1016/j.vetpar.2008.01.042
M Alonso J Torres C Sandoval H Hoste A Aguilar In vitro larval migration and kinetics of exsheathment of Haemonchus contortus larvae exposed to four tropical tanniniferous plant extractsVet. Parasitol.2008153313319https://doi.org/10.1016/j.vetpar.2008.01.042
27. Minho A, Bueno I, Louvandini H, Jackson F, Gennari S, Abdalla A. Effect of Acacia molissima tannin extract on the control of gastrointestinal parasites in sheep. Anim. Feed Sci. Technol. 2008;147:172-181. DOI: https://doi.org/10.1016/j.anifeedsci.2007.09.016
A Minho I Bueno H Louvandini F Jackson S Gennari A Abdalla Effect of Acacia molissima tannin extract on the control of gastrointestinal parasites in sheepAnim. Feed Sci. Technol.2008147172181https://doi.org/10.1016/j.anifeedsci.2007.09.016
28. Domingues L, Giglioti R, Feitosa K, Fantatto R, Rabelo M, Oliveira M, et al. In vitro and in vivo evaluation of the activity of pineapple (Ananas comosus) on Haemonchus contortus in Santa Inês sheep. Vet. Parasitol. 2013;197:263-270. DOI: https://doi.org/10.1016/j.vetpar.2013.04.031
L Domingues R Giglioti K Feitosa R Fantatto M Rabelo M Oliveira In vitro and in vivo evaluation of the activity of pineapple (Ananas comosus) on Haemonchus contortus in Santa Inês sheepVet. Parasitol.2013197263270https://doi.org/10.1016/j.vetpar.2013.04.031
29. Ribeiro V, Avancini C, Gonçalves K, Toigo E, von Poser G. Acaricidal activity of Calea serrata (Asteraceae) on Boophilus microplus and Rhipicephalus sanguineus. Vet. Parasitol. 2008;15:351-354. DOI: https://doi.org/10.1016/j.vetpar.2007.11.007
V Ribeiro C Avancini K Gonçalves E Toigo G von Poser Acaricidal activity of Calea serrata (Asteraceae) on Boophilus microplus and Rhipicephalus sanguineusVet. Parasitol.200815351354https://doi.org/10.1016/j.vetpar.2007.11.007
30. Poolperm S, Jiraungkoorskul W. An Update Review on the Anthelmintic Activity of Bitter Gourd, Momordica charantia. Pharmacogn. Rev. 2017;11:31-34. DOI: https://doi.org/10.4103/phrev.phrev_52_16
S Poolperm W Jiraungkoorskul An Update Review on the Anthelmintic Activity of Bitter Gourd, Momordica charantiaPharmacogn. Rev.2017113134https://doi.org/10.4103/phrev.phrev_52_16
31. Andrade K, Duque D, Jaramillo D. Momordica charantia como alternativa terapéutica en la medicina veterinaria. Rev. Sist. Prod. Agroecol. 2012;3:15-35. DOI: https://doi.org/10.18387/polibotanica.41.6
K Andrade D Duque D Jaramillo Momordica charantia como alternativa terapéutica en la medicina veterinariaRev. Sist. Prod. Agroecol.201231535https://doi.org/10.18387/polibotanica.41.6
32. Grover J, Yadav S. Pharmacological actions and potential uses ofMomordica charantia: A review. Journal of Ethnopharmacology. 2004;93:123-132. DOI: https://doi.org/10.1016/j.jep.2004.03.035
J Grover S. Yadav Pharmacological actions and potential uses ofMomordica charantia : A reviewJournal of Ethnopharmacology200493123132https://doi.org/10.1016/j.jep.2004.03.035
35. Giron L, Freire V, Alonzo A, Caceres A. Ethnobotanical survey of the medicinal flora used by the Caribs of Guatemala. Journal of Ethnopharmacology . 1991;34:173-187. DOI: https://doi.org/10.1016/0378-8741(91)90035-C
L Giron V Freire A Alonzo A Caceres Ethnobotanical survey of the medicinal flora used by the Caribs of GuatemalaJournal of Ethnopharmacology199134173187https://doi.org/10.1016/0378-8741(91)90035-C
36. Lans C, Brown G. Observations on ethnoveterinary medicines in Trinidad and Tobago. Preventive Veterinary Medicine. 1998;35:125-142. DOI: https://doi.org/10.1016/S0167-5877(97)00055-X
C Lans G Brown Observations on ethnoveterinary medicines in Trinidad and TobagoPreventive Veterinary Medicine199835125142https://doi.org/10.1016/S0167-5877(97)00055-X
38. Achi YL, Zinsstag J, Yao K, Yeo N, Dorchies P, Jacquiet P. Host specificity of Haemonchus spp. for domestic ruminants in the savanna in northern Ivory Coast. Vet. Parasitol. 2003;116:151-158. DOI: https://doi.org/10.1016/s0304-4017(03)00258-9
YL Achi J Zinsstag K Yao N Yeo P Dorchies P Jacquiet Host specificity of Haemonchus spp. for domestic ruminants in the savanna in northern Ivory CoastVet. Parasitol.2003116151158https://doi.org/10.1016/s0304-4017(03)00258-9
39. Vieira L, Cavalcante A, Pereira M, Dantas L, Ximenes L. Evaluation of anthelmintic efficacy of plants available in Ceara State, north-east Brazil, for the control of goat gastrointestinal nematodes. Rev. Med. Vet [Internet]. 1999;150:447-452. Available from: https://www.revmedvet.com/artdes-us.php?id=53
L Vieira A Cavalcante M Pereira L Dantas L Ximenes Evaluation of anthelmintic efficacy of plants available in Ceara State, north-east Brazil, for the control of goat gastrointestinal nematodesRev. Med. Vet1999150447452https://www.revmedvet.com/artdes-us.php?id=53
40. Githiori J, Athanasiadou S, Thamsborg S. Use of plants in novel approaches for control of gastrointestinal helminths in livestock with emphasis on small ruminants. Vet. Parasitol. 2006;139:308-320. DOI: https://doi.org/10.1016/j.vetpar.2006.04.021
J Githiori S Athanasiadou S Thamsborg Use of plants in novel approaches for control of gastrointestinal helminths in livestock with emphasis on small ruminantsVet. Parasitol.2006139308320https://doi.org/10.1016/j.vetpar.2006.04.021
41. Mihranian V, Abou C. Extraction, detection, and estimation of cucurbitin in Cucurbita seeds, J Lloydia [Internet]. 1968;31:23-29. Available from: https://eurekamag.com/research/014/472/014472546.php
V Mihranian C Abou Extraction, detection, and estimation of cucurbitin in Cucurbita seedsJ Lloydia1968312329https://eurekamag.com/research/014/472/014472546.php
42. Okabe H, Miyahara Y, Yamauchi T. Studies on the constituents of Momordica Charantia L. Isolation and characterisation of momordicosides A and B, glycosides of a pentahydroxy-cucurbitane triterpene. Chem Pharm Bull. 1980;28:2753-2762. DOI: https://doi.org/10.1248/cpb.28.2753
H Okabe Y Miyahara T Yamauchi Studies on the constituents of Momordica Charantia L. Isolation and characterisation of momordicosides A and B, glycosides of a pentahydroxy-cucurbitane triterpeneChem Pharm Bull.19802827532762https://doi.org/10.1248/cpb.28.2753
45. Michael B, Meinke P, Shoop W. Comparison of ivermectin, doramectin, selamectin, and eleven intermediates in a nematode larval development assay. J Parasitol. 2001;87:692-696. https://doi.org/10.1645/00223395(2001)087[0692:coidsa]2.0.co;2
B Michael P Meinke W Shoop Comparison of ivermectin, doramectin, selamectin, and eleven intermediates in a nematode larval development assayJ Parasitol.200187692696https://doi.org/10.1645/00223395(2001)087[0692:coidsa]2.0.co;2
46. Bizimenyera E, Githiori J, Eloff J, Swan G. In vitro activity of Peltophorum africanum Sond. (Fabaceae) extracts on the egg hatching and larval development of the parasitic nematode Trichostrongylus colubriformis. Vet. Parasitol. 2006;142:336-343. DOI: https://doi.org/10.1016/j.vetpar.2006.06.013
E Bizimenyera J Githiori J Eloff G Swan In vitro activity of Peltophorum africanum Sond. (Fabaceae) extracts on the egg hatching and larval development of the parasitic nematode Trichostrongylus colubriformisVet. Parasitol.2006142336343https://doi.org/10.1016/j.vetpar.2006.06.013
47. Coles G, Bauer C, Borgsteede F, Geerts S, Klei T, Taylor M, et al. World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection ofanthelmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 1992;44:35-44. DOI: https://doi.org/10.1016/0304-4017(92)90141-u
G Coles C Bauer F Borgsteede S Geerts T Klei M Taylor World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection ofanthelmintic resistance in nematodes of veterinary importanceVet. Parasitol.1992443544https://doi.org/10.1016/0304-4017(92)90141-u
48. Powers KG, Wood I, Eckert J, Gibson T, Smith H. World associations of the advancement of veterinary parasitology (WAAVP) guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine and ovine). Vet. Parasitol. 1982;10:265-284. DOI: https://doi.org/10.1016/0304-4017(82)90078-4
KG Powers I Wood J Eckert T Gibson H Smith World associations of the advancement of veterinary parasitology (WAAVP) guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine and ovine)Vet. Parasitol.198210265284https://doi.org/10.1016/0304-4017(82)90078-4
49. Costa J, Nascimento E, Campos A, Rodrigues F. Antibacterial activity of Momordica charantia (Curcubitaceae) extracts and fractions. J Basic Clin Pharm. 2010;2:45-51. DOI: http://www.ncbi.nlm.nih.gov/pmc/articles/pmc3979203/
J Costa E Nascimento A Campos F Rodrigues Antibacterial activity of Momordica charantia (Curcubitaceae) extracts and fractionsJ Basic Clin Pharm.201024551http://www.ncbi.nlm.nih.gov/pmc/articles/pmc3979203/
50. Yaldiz G, Sekeroglu N, Kulak M, Demirkol G. Antimicrobial activity and agricultural properties of bitter melon (Momordica charantia L.) grown in northern parts of Turkey: A case study for adaptation. Nat Prod Res. 2015;29:543-545. DOI: https://doi.org/10.1080/14786419.2014.949706
G Yaldiz N Sekeroglu M Kulak G Demirkol Antimicrobial activity and agricultural properties of bitter melon (Momordica charantia L.) grown in northern parts of Turkey: A case study for adaptationNat Prod Res.201529543545https://doi.org/10.1080/14786419.2014.949706
51. Pitchakarn P, Ogawa K, Suzuki S, Takahashi S, Asamoto M, Chewonarin T. Momordica charantia leaf extract suppresses rat prostate cancer progression in vitro and in vivo. Cancer Sci. 2010;101:2234-2240. DOI: https://doi.org/10.1111/j.1349-7006.2010.01669.x
P Pitchakarn K Ogawa S Suzuki S Takahashi M Asamoto T Chewonarin Momordica charantia leaf extract suppresses rat prostate cancer progression in vitro and in vivoCancer Sci.201010122342240https://doi.org/10.1111/j.1349-7006.2010.01669.x
52. Shobha C, Vishwanath P, Suma M, Prashant A, Rangaswamy C, Gowdappa B. In vitro anti-cancer activity of ethanolic extract of Momordica charantia on cervical and breast cancer cell lines. J of Health Allied Sci [Internet] 2015;4:210-217. Available from: https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.4103%2F2278-344X.167649
C Shobha P Vishwanath M Suma A Prashant C Rangaswamy B Gowdappa In vitro anti-cancer activity of ethanolic extract of Momordica charantia on cervical and breast cancer cell linesJ of Health Allied Sci20154210217https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.4103%2F2278-344X.167649
53. Tahira S, Hussain F. Antidiabetic evaluation of Momordica charantia L fruit extracts. West Indian Med J. 2014; 63:294-299. DOI: https://doi.org/10.7727/wimj.2013.180
S Tahira F Hussain Antidiabetic evaluation of Momordica charantia L fruit extractsWest Indian Med J.201463294299https://doi.org/10.7727/wimj.2013.180
54. Perumal V, Khoo W, Abdul-Hamid A, Ismail A, Saari K, Murugesu S. Evaluation of antidiabetic properties of Momordica charantia in streptozotocin Induced diabetic rats using metabolomics approach. J Food Res [Internet] 2015;22:1298-1306. Available from: https://www.researchgate.net/publication/282198654_Evaluation_of_antidiabetic_properties_of_Momordica_charantia_in_streptozotocin_induced_diabetic_rats_using_metabolomics_approach
V Perumal W Khoo A Abdul-Hamid A Ismail K Saari S Murugesu Evaluation of antidiabetic properties of Momordica charantia in streptozotocin Induced diabetic rats using metabolomics approachJ Food Res20152212981306https://www.researchgate.net/publication/282198654_Evaluation_of_antidiabetic_properties_of_Momordica_charantia_in_streptozotocin_induced_diabetic_rats_using_metabolomics_approach
55. Meera S, Nagarjuna C. Antistress and immunomodulatory activity of aqueous extract of Momordica charantia. Pharmacogn Mag [Internet]. 2009; 5:69-73. Available from: https://www.researchgate.net/publication/287479315_Antistress_and_immunomodulatory_activity_of_aqueous_extract_of_Momordica_charantia
S Meera C Nagarjuna Antistress and immunomodulatory activity of aqueous extract of Momordica charantiaPharmacogn Mag200956973https://www.researchgate.net/publication/287479315_Antistress_and_immunomodulatory_activity_of_aqueous_extract_of_Momordica_charantia
56. Gupta M, Sharma S, Bhadauria R. In vitro efficacy of Momordica charantia extracts against phytopathogenic fungi, Fusarium oxysporum. J Biopesticides [Internet] 2016;9:8-22. Available from: https://www.researchgate.net/publication/304956887_In_vitro_efficacy_of_Momordica_charantia_extracts_against_phytopathogenic_fungi_Fusarium_oxysporum
M Gupta S Sharma R Bhadauria In vitro efficacy of Momordica charantia extracts against phytopathogenic fungi, Fusarium oxysporumJ Biopesticides20169822https://www.researchgate.net/publication/304956887_In_vitro_efficacy_of_Momordica_charantia_extracts_against_phytopathogenic_fungi_Fusarium_oxysporum
57. Wang S, Zheng Y, Xiang F, Li S, Yang, G. Antifungal activity of Momordica charantia seed extracts toward the pathogenic fungus Fusarium solani L. J Food Drug Anal [Internet]. 2016; 24:881-887. Available from: https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.jfda.2016.03.006
Zheng Y Wang S Li S Xiang F G Yang Antifungal activity of Momordica charantia seed extracts toward the pathogenic fungus Fusarium solani LJ Food Drug Anal201624881887https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.jfda.2016.03.006
58. Puri M, Kaur I, Kanwar R, Gupta R, Chauhan A, Kanwar J. Ribosome inactivating proteins (RIPs) from Momordica charantia for anti viral therapy. Curr Mol Med. 2009;9:1080-1094. DOI: https://doi.org/10.2174/156652409789839071
M Puri I Kaur R Kanwar R Gupta A Chauhan J Kanwar Ribosome inactivating proteins (RIPs) from Momordica charantia for anti viral therapyCurr Mol Med.2009910801094https://doi.org/10.2174/156652409789839071
59. Tjokropranoto R, Nathania M. Anthelmintic effect of ethanol extract of pare leaf (Momordica charantia L.) against female Ascaris suum worm in vitro. J Med Planta [Internet]. 2011;1:33-39. Available from: https://www.neliti.com/publications/245875/anthelmintic-effect-of-ethanol-extract-of-pare-leaf-momordica-charantia-l-agains
R Tjokropranoto M Nathania Anthelmintic effect of ethanol extract of pare leaf (Momordica charantia L.) against female Ascaris suum worm in vitroJ Med Planta201113339https://www.neliti.com/publications/245875/anthelmintic-effect-of-ethanol-extract-of-pare-leaf-momordica-charantia-l-agains
60. Chastity C, Yuwono K, Utami U, PralaAyu A, Priscillah W, Sutrisna E. The anthelmintics effect of Momordica charantia L. leaves and Andrographis paniculata Ness. from Indonesia. Int J Ayurveda Pharm Res [Internet]. 2015; 3:33-9. Available from: https://ijapr.in/index.php/ijapr/article/view/127
C Chastity K Yuwono U Utami A PralaAyu W Priscillah E Sutrisna The anthelmintics effect of Momordica charantia L. leaves and Andrographis paniculata Ness. from IndonesiaInt J Ayurveda Pharm Res201533339https://ijapr.in/index.php/ijapr/article/view/127
61. Shahadat H.; Mostofa M.; Mamun, M.; Hoque, M.; Awal, M. Comparative efficacy of korolla (Momordica charantia ) extract and Ivermec® pour on with their effects on certain blood parameters and body weight gain in indigenous chicken infected with Ascaridia galli. Bangladesh J Vet Med. 2008;6:153-158. DOI: https://doi.org/10.1016/j.vetpar.2007.05.015
H. Shahadat M. Mostofa M. Mamun M. Hoque M. Awal Comparative efficacy of korolla (Momordica charantia ) extract and Ivermec® pour on with their effects on certain blood parameters and body weight gain in indigenous chicken infected with Ascaridia galliBangladesh J Vet Med.20086153158https://doi.org/10.1016/j.vetpar.2007.05.015
62. Alam M, Alam K, Begum N, Amin M. Comparative efficacy of different herbal and modern anthelmintics against gastrointestinal nematodiasis in fowl. J Biol Res [Internet]. 2014; 2:145-148. Available from: https://www.sciencepubco.com/index.php/IJBR/article/view/3584
M Alam K Alam N Begum M Amin Comparative efficacy of different herbal and modern anthelmintics against gastrointestinal nematodiasis in fowlJ Biol Res20142145148https://www.sciencepubco.com/index.php/IJBR/article/view/3584
63. USDA Composition of Foods Raw, Processed, Prepared USDA. National Nutrient Database for Standard Reference, Release 21, US Department of Agriculture, Maryland; 2008.
USDA Composition of Foods Raw, Processed, Prepared USDA National Nutrient Database for Standard Reference, Release 21US Department of AgricultureMaryland2008
64. Wang L, Wang M, Li Q, Cai T, Jiang W. Partial properties of an aspartic protease in bitter gourd (Momordica charantia L.) fruit and its activation by heating. Food Chem. 2008;108:496-502. DOI: https://doi.org/10.1016/j.foodchem.2007.10.085
L Wang M Wang Q Li T Cai W Jiang Partial properties of an aspartic protease in bitter gourd (Momordica charantia L.) fruit and its activation by heatingFood Chem.2008108496502https://doi.org/10.1016/j.foodchem.2007.10.085
65. Islam S, Jalaluddin M, Hettiarachchy N. Bio-active compounds of bitter melongenotypes (Momordica charantia L.) in relation to their physiological functions. Functional Foods in Health & Disease. 2011; 2:61-74. DOI:https://doi.org/10.31989/ffhd.v1i2.139
S Islam M Jalaluddin N Hettiarachchy Bio-active compounds of bitter melongenotypes (Momordica charantia L.) in relation to their physiological functionsFunctional Foods in Health & Disease201126174https://doi.org/10.31989/ffhd.v1i2.139
66. Bauri R, Tigga M, Kullu S. A review on use of medicinal plants to control parasites. Indian J Nat Prod Resour. [Internet] 2015; 6:268-277. Available from: https://www.researchgate.net/publication/292161550_A_review_on_use_of_medicinal_plants_to_control_parasites
R Bauri M Tigga S Kullu A review on use of medicinal plants to control parasitesIndian J Nat Prod Resour.20156268277https://www.researchgate.net/publication/292161550_A_review_on_use_of_medicinal_plants_to_control_parasites
67. Aleman Y, Ferreira L, Pino O, Dias M, Roque E, de Souza A. Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus. Industrial Crops and Products. 2015;76:647-652. DOI: https://doi.org/10.1016/j.indcrop.2015.07.056
Y Aleman L Ferreira O Pino M Dias E Roque A de Souza Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortusIndustrial Crops and Products201576647652https://doi.org/10.1016/j.indcrop.2015.07.056
68. Cavalcante G, de Morais S, Andre W, Ribeiro W, Rodrigues A, De Lira F, et al. Chemical composition and in vitro activity of Qªª (Ait.) latex on Haemonchus contortus. Vet. Parasitol. 2016;226:22-25. DOI: https://doi.org/10.1016/j.vetpar.2016.06.012
G Cavalcante S de Morais W Andre W Ribeiro A Rodrigues F De Lira Chemical composition and in vitro activity of Qªª (Ait.) latex on Haemonchus contortusVet. Parasitol.20162262225https://doi.org/10.1016/j.vetpar.2016.06.012
69. Zhu L, Dai J, Yang L, Qiu J. In vitro ovicidal and larvicidal activity of the essential oil of Artemisia lancea against Haemonchus contortus (Strongylida). Vet Parasitol. 2013;195:112-117. DOI: https://doi.org/10.1016/j.vetpar.2012.12.050
L Zhu J Dai L Yang J Qiu In vitro ovicidal and larvicidal activity of the essential oil of Artemisia lancea against Haemonchus contortus (Strongylida)Vet Parasitol.2013195112117https://doi.org/10.1016/j.vetpar.2012.12.050
70. Minho A, Domingues L, Gainza Y, Figueiredo A, Boligon A, Domingues R, et al. In vitro screening of plant extract on Haemonchus contortus and Rhipicephalus (Boophilus) microplus. Journal of Essential Oil Research. 2020; 32:269-278. DOI: https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.1080%2F10412905.2020.1746414
A Minho L Domingues Y Gainza A Figueiredo A Boligon R Domingues In vitro screening of plant extract on Haemonchus contortus and Rhipicephalus (Boophilus) microplusJournal of Essential Oil Research202032269278https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.1080%2F10412905.2020.1746414
71. Carvalho C, Chagas A, Cotinguiba F, Furlan M, Brito L, Chaves F, et al. The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis. Vet Parasitol. 2012;183:260-268. DOI: https://doi.org/10.1016/j.vetpar.2011.07.051
C Carvalho A Chagas F Cotinguiba M Furlan L Brito F Chaves The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensisVet Parasitol.2012183260268https://doi.org/10.1016/j.vetpar.2011.07.051
72. Ferreira L, Benincasa B, Fachin A, Franca S, Contini S, Chagas A, et al. Thymus vulgaris L. essential oil and its main component thymol: Anthelmintic effects against Haemonchus contortus from sheep. Vet Parasitol, 2016; 228:70-76. DOI: https://doi.org/10.1016/j.vetpar.2016.08.011
L Ferreira B Benincasa A Fachin S Franca S Contini A Chagas Thymus vulgaris L. essential oil and its main component thymol: Anthelmintic effects against Haemonchus contortus from sheepVet Parasitol20162287076https://doi.org/10.1016/j.vetpar.2016.08.011
73. Kanojiya D, Shanker D, Sudan V, Jaiswal A, Parashar R. Anthelmintic activity of Ocimum sanctum leaf extract against ovine gastrointestinal activity of nematodes in India. Research in Veterinary Science. 2015;99:165-170. DOI: https://doi.org/10.1016/j.rvsc.2015.01.017
D Kanojiya D Shanker V Sudan A Jaiswal R Parashar Anthelmintic activity of Ocimum sanctum leaf extract against ovine gastrointestinal activity of nematodes in IndiaResearch in Veterinary Science201599165170https://doi.org/10.1016/j.rvsc.2015.01.017
74. Eguale T, Tilahun G, Debella A, Feleke A, Makonnen E. Haemonchus contortus: In vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helix. Experimental Parasitology. 2007; 116:340-345. DOI: https://doi.org/10.1016/j.exppara.2007.01.019
T Eguale G Tilahun A Debella A Feleke E Makonnen Haemonchus contortus: In vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helixExperimental Parasitology2007116340345https://doi.org/10.1016/j.exppara.2007.01.019
75. Davuluri T, Chennuru S, Pathipati M, Krovvidi S, Rao G. In Vitro Anthelmintic Activity of Three Tropical Plant Extracts on Haemonchus contortus. Acta Parasit. 2019; 65:11-18. DOI: http://dx.doi.org/10.2478/s11686-019-00116-x
T Davuluri S Chennuru M Pathipati S Krovvidi G Rao In Vitro Anthelmintic Activity of Three Tropical Plant Extracts on Haemonchus contortusActa Parasit.2019651118http://dx.doi.org/10.2478/s11686-019-00116-x
66. Mengistu G, Hoste H, Karonen M, Salminen J, Hendriks W, Pellikaan W. The in vitro anthelmintic properties of browse plant species against Haemonchus contortus is determined by the polyphenol content and composition. Vet Parasitol 2017; 237:110-116. DOI: https://doi.org/10.1016/j.vetpar.2016.12.020
G Mengistu H Hoste M Karonen J Salminen W Hendriks W Pellikaan The in vitro anthelmintic properties of browse plant species against Haemonchus contortus is determined by the polyphenol content and compositionVet Parasitol2017237110116https://doi.org/10.1016/j.vetpar.2016.12.020
77. Shan B, Xie J, Zhu J, Peng Y. Ethanol modified supercritical carbon dioxide extraction of flavonoids from Momordica charantia L. and its antioxidant activity. Food Bioproducts Process. 2012;90:579-587. DOI: https://doi.org/10.1016/j.fbp.2011.09.004
B Shan J Xie J Zhu Y Peng Ethanol modified supercritical carbon dioxide extraction of flavonoids from Momordica charantia L. and its antioxidant activityFood Bioproducts Process.201290579587https://doi.org/10.1016/j.fbp.2011.09.004
78. Chaudary F, Qayyum M, Miller J. Development and survival of Haemonchus contortus infective larvae derived from sheep faeces under sub-tropical conditions in the Potohar region of Pakistan. Trop. Anim. Health Prod. 2008; 40:85-92. DOI: https://doi.org/10.1007/s11250-007-9037-x
F Chaudary M Qayyum J Miller Development and survival of Haemonchus contortus infective larvae derived from sheep faeces under sub-tropical conditions in the Potohar region of PakistanTrop. Anim. Health Prod.2008408592https://doi.org/10.1007/s11250-007-9037-x
79. Stepek G, Behnke J, Buttle D, Duce I. Natural plant cysteine proteinases as anthelmintic? Trends Parasitol. 2004;20: 322-327. DOI: https://doi.org/10.1016/j.pt.2004.05.003
G Stepek J Behnke D Buttle I Duce Natural plant cysteine proteinases as anthelmintic?Trends Parasitol.200420322327https://doi.org/10.1016/j.pt.2004.05.003
80. Buttle D, Behnke J, Bartley Y, Elsheikha H, Bartley D, Garnett M, et al. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus. Parasite Vector. 2011; 4:1-11. DOI: https://doi.org/10.1186/1756-3305-4-36
D Buttle J Behnke Y Bartley H Elsheikha D Bartley M Garnett Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortusParasite Vector.20114111https://doi.org/10.1186/1756-3305-4-36
81. Mansfield L, Gamble H, Fetterer R. Characterization of the eggshell of Haemonchus contortus-I. Structural components. Comp. Biochem. Physiol. 1992;103: 681-686. DOI: https://doi.org/10.1016/0305-0491(92)90390-D
L Mansfield H Gamble R Fetterer Characterization of the eggshell of Haemonchus contortus-I. Structural componentsComp. Biochem. Physiol.1992103681686https://doi.org/10.1016/0305-0491(92)90390-D
82. Beloin N, Gbeassor M, Akpagana K, Hudson J, Soussa K, Koumaglo K, et al. Ethnomedicinal uses of Momordica charantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activity. Journal of Ethnopharmacology. 2005; 96:49-55. DOI: https://doi.org/10.1016/j.jep.2004.08.009
N Beloin M Gbeassor K Akpagana J Hudson K Soussa K Koumaglo Ethnomedicinal uses of Momordica charantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activityJournal of Ethnopharmacology2005964955https://doi.org/10.1016/j.jep.2004.08.009
83. Ling B, Wang G, Ya J, Zhang M, Liang G. Antifeedant activity and active ingredients against Plutella xylostella from Momordica charantia leaves. Agricultural Sciences in China. 2008;7:1466-1473. DOI: https://doi.org/10.1016/S1671-2927(08)60404-6
B Ling G Wang J Ya M Zhang G Liang Antifeedant activity and active ingredients against Plutella xylostella from Momordica charantia leavesAgricultural Sciences in China2008714661473https://doi.org/10.1016/S1671-2927(08)60404-6
84. Islam K, Farjana T, Begum N, Mondal M. In vitro efficacy of some indigenous plants on the inhibition of development of eggs of Ascaridia galli (Digenia: Nematoda). Bangladesh J. Vet. Med. 2008; 6:159-167. DOI: http://dx.doi.org/10.3329/bjvm.v6i2.2330
K Islam T Farjana N Begum M Mondal In vitro efficacy of some indigenous plants on the inhibition of development of eggs of Ascaridia galli (Digenia: Nematoda)Bangladesh J. Vet. Med.20086159167http://dx.doi.org/10.3329/bjvm.v6i2.2330