ELABORACIÓN DE ANDAMIOS POROSOS OSTEOINDUCTIVOS DE POLI (ÁCIDO L-LÁCTICO)/QUITOSANO PARA LA REGENERACIÓN DE TEJIDO ÓSEO

Liz Solange Correa; Fabio Zuluaga; Carlos Valencia; Jorge Eduardo Godoy

doi:10.17533/udea.rcm.22873

Authors

Liz Solange Correa Universidad del Valle
Fabio Zuluaga Universidad del Valle
Carlos Valencia Universidad del Valle
Jorge Eduardo Godoy Universidad del Valle

DOI:

https://doi.org/10.17533/udea.rcm.22873

Keywords:

Chitosan, Poly (L-lactide), in vivo test, Aspergillus níger, Porous Scaffolds

Abstract

In this work the extraction of chitosan (CHT) was made from the fungus Aspergillus niger whose yield was 0.6% on dry initial amount. The CHT was characterized by infrared spectroscopy (FT-IR) and NMR-1H) Nuclear Magnetic Resonance. Its molecular weight (200,605 g / mol), was determined by capillary viscosimetry and its degree of deacetylation (68.51%) was determined by elemental analysis techniques and potentiometric titration. Scaffolds of poly (L-lactide) (PLLA)/CHT were prepared by depositing CHT through the pores of the PLLA scaffolds, previously created by the melt molding technique. Morphology and pore architecture of these scaffolds were evaluated by Scanning Electron Microscopy (SEM) and found the existence of CHT within the pores by fluorescence spectroscopy technique. These material were tested for compression and hydrolytic degradation porosity in order to simulate their behavior within the organism by in vivo assays. These in vivo in rabbits tests showed that the porous blocks of PLLA/CHT implanted into critical size effects in rabbits showed adequate biointegration, showing scar bone regeneration process.

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Author Biographies

Liz Solange Correa, Universidad del Valle

Master's Student, School of Materials Engineering, Universidad del Valle, Cali, Colombia

Fabio Zuluaga, Universidad del Valle

Professor, Department of Chemistry, Universidad del Valle, Cali, Colombia

Carlos Valencia, Universidad del Valle

PhD Student, School of Dentistry, Universidad del Valle, Cali, Colombia

Jorge Eduardo Godoy, Universidad del Valle

Master's Student, School of Materials Engineering, Universidad del Valle, Cali, Colombia

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