LA BIOINTEGRACIÓN DE LOS FOSFATOS DE CALCIO, UN PROBLEMA DE INTERFAZ

Carlos H. Valencia; Mario A. Ortiz; Sandra Arce

doi:10.17533/udea.rcm.19426

Authors

Carlos H. Valencia Universidad del Valle
Mario A. Ortiz Universidad Del Valle
Sandra Arce Autonomous university of Occident

DOI:

https://doi.org/10.17533/udea.rcm.19426

Keywords:

Tricalcium phosphate, Chitosan, Bone regeneration, Biomaterials

Abstract

Calcium phosphate has a great potential as bone substitute in regenerative techniques due to its

bioactivity and fast resorption rate. However, it is necessary to understand its biological

integration dynamics.

This paper evaluates this problem using histochemistry and immunohistochemistry techniques.

All protocols used in this work were approved by Animal Ethics Committee at Universidad del

Valle. Therefore, the aim of this study was evaluate the biointegration process of a selected

Calcium phosphate/chitosan bio-compound implanted in critical-size defects made by surgical techniques in parietal bone of male Wistar rats.

Samples were collected at 20, 40 and 60 days after implantation and were studied using histochemical (hematoxilin-eosin, Masson trichrome staining and Gomori Stain for reticulin) and immunohistochemistry techniques (using primary antibodies against human smooth muscle actin

and tartrate-resistant acid phosphatase).

The histological evaluation of the samples shows a strong inflammatory reaction at initial stages

that decreases in final stages. At the same time, angiogenesis, collagen synthesis, osteoclastogenesis y osteoblastogenesis may be described. The implanted bio-compound isincorporated in the surrounding tissue by a collagen and blood vessels network. TRAP positive cells (osteoclast-like cells) can be found around the biocompound.

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Author Biographies

Carlos H. Valencia, Universidad del Valle

Oral and Reconstructive Implantologist. Faculty of Health. Universidad del Valle. Cali, Colombia

Mario A. Ortiz, Universidad Del Valle

MSc. Faculty of Health. Universidad Del Valle. Cali, Colombia

Sandra Arce, Autonomous university of Occident

MSc. Faculty of Engineering. Autonomous university of Occident. Cali, Colombia

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