Efecto de la luz en la supervivencia de Caenorhabditis elegans

Marcela  Gómez Garzón; Juan Pablo Cobos; Juan Diego Castillo

doi:10.17533/udea.hm.v15n1a02

Authors

Marcela Gómez Garzón Fundación Universitaria de Ciencias de la Salud https://orcid.org/0000-0003-0584-8783
Juan Pablo Cobos Fundación Universitaria Ciencias de la Salud https://orcid.org/0009-0005-0049-1457
Juan Diego Castillo Fundación Universitaria Ciencias de la Salud https://orcid.org/0009-0004-8048-5331

DOI:

https://doi.org/10.17533/udea.hm.v15n1a02

Keywords:

Caenorhabditis elegans, light, survival

Abstract

Introduction: Sleep is essential for human health, and it is influenced by internal circadian rhythms that can be adjusted by external cues such as light. The ne-matode Caenorhabditis elegans is an invertebrate model that shows similarities to mammalian-sleep patterns. However, excessive light exposure can negatively affect stress induced sleep (SIS). Objective: To eva-luate the effect of blue light (LED) and medium wave UV (UVB) on the survival of C. elegans and compare with the circadian cycle of 12 h white light exposure. Methods: An experiment was carried out using L1 larvae of C. elegans N2 cultured on NGM agar with a food source of E. coli OP50, and subjected to dark-ness, white, LED and UVB Lights in periods of 12 h and 24 h. Results: At the end of the experiment, an increase in mortality due to phototoxicity was evident, demonstrated by a lower survival rate in the larvae ex-posed for 24 h, when compared to the larvae that re-mained in permanent darkness, a natural condition of the parasite. Conclusions: These results show us that the longer the exposure photoperiod, the shorter the survival of C. elegans.

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Author Biographies

Marcela Gómez Garzón, Fundación Universitaria de Ciencias de la Salud

Facultad de Enfermería. Fundación Universitaria Ciencias de la Salud. Semillero SIMIC.

Juan Pablo Cobos, Fundación Universitaria Ciencias de la Salud

Estudiante Facultad de Enfermería. Fundación Universitaria Ciencias de la Salud. Semillero SIMIC

Juan Diego Castillo, Fundación Universitaria Ciencias de la Salud

Estudiante Facultad de Enfermería. Fundación Universitaria Ciencias de la Salud. Semillero SIMIC

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