Detection of naturally infected Anopheles mosquitoes by Plasmodium spp. in Puerto Libertador, Cordoba, Colombia

Authors

  • Natalí Álvarez University of Antioquia
  • Doris A. Rosero University of Antioquia
  • Giovan F Gómez University of Antioquia
  • Margarita M Correa University of Antioquia

DOI:

https://doi.org/10.17533/udea.hm.12647

Keywords:

Anopheles, Colombia, Enzyme-Linked Immumosorbent Assay, malaria, Plasmodium, polymerase chain reaction

Abstract

INTRODUCTION
Anopheles species vary in their ability to transmit malaria parasites; therefore, it is important to conduct studies directed to determine the species present in malaria endemic regions and establish their role in transmission.
OBJECTIVE
To determine the Anopheles species involved in malaria transmission in the locality of Juan José, Puerto Libertador, Cordoba, Colombia.
MATERIALS AND METHODS
Natural infectivity by Plasmodium vivax VK210, P. vivax VK247 and Plasmodium falciparum was evaluated by ELISA using pools of the head and thorax of five mosquitoes of the same species per well. ELISA results with values greater then the cutoff point were confirmed by a second ELISA and nested PCR.
RESULTS
From 2,054 specimens evaluated by ELISA, 2,038 were Anopheles (Nyssorhynchus) nuneztovari Gabaldon; from these, three specimens were found infected, two with P. vivax VK210 and one with P. falciparum, representing an infection rate of 0.15%. Of 16 An (Nys.) darlingi Root evaluated, none was found infected.
CONCLUSIONS
An. nuneztovari s.l., and An. darlingi, two main malaria vectors of Colombia were present in San Juse, Puerto Libertador; however, only An. nuneztovari s.l., was found infected, indicating its role in transmission in this locality. Further sampling in Juan Jose will allow for a better understanding of the role of An. darlingi in transmission. This information will contribute to the desing and implementation of tergeted and effective control measures. 
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Author Biographies

Natalí Álvarez, University of Antioquia

Microbiology and Bioanalysis student, Molecular Microbiology Group, School of Microbiology, University of Antioquia. Medellin Colombia.

Doris A. Rosero, University of Antioquia

Microbiologist and Bioanalyst, Master's student in Biology, Molecular Microbiology Group, School of Microbiology, Universidad de Antioquia, Medellín, Colombia.

Giovan F Gómez, University of Antioquia

Microbiologist and Bioanalyst, PhD Student in Basic Biomedical Sciences, Molecular Microbiology Group, School of Microbiology, University of Antioquia, Medellín, Colombia.

Margarita M Correa, University of Antioquia

PhD, Professor, Molecular Microbiology Group, School of Microbiology, University of Antioquia, Medellín, Colombia.

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Published

2012-08-25

How to Cite

Álvarez, N., Rosero, D. A., Gómez, G. F., & Correa, M. M. (2012). Detection of naturally infected Anopheles mosquitoes by Plasmodium spp. in Puerto Libertador, Cordoba, Colombia. Hechos Microbiológicos, 2(2), 27–35. https://doi.org/10.17533/udea.hm.12647

Issue

Vol. 2 No. 2 (2011)

Section

Artículos de investigación original
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