Utilidad de las técnicas moleculares aplicadas al diagnóstico de Babesia bovis y Babesia bigemina en Ganado Bovino: Revisión Sistemática

Natalia María Guevara A.; Lina María Rivas J.; Leonardo Alberto Ríos O.

doi:10.17533/udea.hm.12651

Authors

Natalia María Guevara A. University of Antioquia
Lina María Rivas J. University of Chile
Leonardo Alberto Ríos O. University of Antioquia

DOI:

https://doi.org/10.17533/udea.hm.12651

Keywords:

Babesia, western blotting, cattle, diagnostic techniques and procedures, polimerase chain reaction

Abstract

INTRODUCTION

Several microscopic, serological and molecular methods are available to diagnosis babesiosis; however, molecular techniques have gained special interest because they allow detection of genetic material at low concentrations, and sometimes it is possible to discriminate between species.

OBJECTIVE

To describe the utility of the main molecular techniques applied to diagnosis of infections by Babesia bovis and B. bigemina in cattle.

MATERIALS AND METHODS

We conducted a systematic review of literature published between 2006 and 2011 in Pubmed, ScienceDirect and Springer Link databases using MeSH terms: Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Molecular Diagnostic Techniques, Western Blotting, Microarray Analysis, Cattle, Babesia and Babesia bovis.

RESULTS

A total of 299 papers were obtained, 28 of them were selected according to the inclusion criteria. Molecular techniques used were PCR and its variants and RLB, to a lesser extent LAMP and Western Blot were used. A brief description of each technique, its application in the diagnosis of infections by B. bovis and B. bigemina in cattle and some aspects to be consider for successful implementation were described; in addition, molecular markers, extraction methods of nucleic acids used and geographic discrimination in the use of the techniques were also described.

CONCLUSIONS

Molecular techniques have the ability to detect infections by B. bovis and B. bigemina with high specificity. In addition, they allow performing studies on the biology of these miccroorganisms, as well as epidemiological, phylogenetic and immunization studies.

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Author Biographies

Natalia María Guevara A., University of Antioquia

Microbiologist and Bioanalyst. Master's student in Microbiology and Bioanalysis, Hematology emphasis. University of Antioquia.

Lina María Rivas J., University of Chile

Microbiologist and Bioanalyst. Student Master in Biological and Medical Sciences Mention Microbiology. University of Chile. Santiago- Chile.

Leonardo Alberto Ríos O., University of Antioquia

Associate professor. Veterinary Microbiology Research Group. School of Microbiology. University of Antioquia.

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