Utilidad diagnóstica de la amplificación isotérmica con la formación de asas (LAMP) para la detección de ADN de citomegalovirus, Epstein Barr y herpes simple 1 y 2 en muestras biológicas: revisión sistemática

Olga L.  Rincón Caballero; Beatriz H.  Aristizábal Bernal

doi:10.17533/udea.hm.5223

Authors

Olga L. Rincón Caballero Pablo Tobon Uribe Hospital
Beatriz H. Aristizábal Bernal Pablo Tobon Uribe Hospital

DOI:

https://doi.org/10.17533/udea.hm.5223

Keywords:

cytomegalovirus, human herpesvirus 1, human herpesvirus 2, human herpesvirus 4, Epstein Barr virus, loopmediated isothermal amplification (LAMP), nucleic acid amplification techniques

Abstract

Introduction

Viral infections cause significant morbidity and mortality in immunocompromised and non-immunocompromised individuals. The completion of an early etiologic diagnosis offers great advantages in the treatment of patients, as appropriate and timely antiviral treatment may improve prognosis. This article demonstrates the diagnostic utility of the Loop-mediated isothermal amplification (LAMP) assay for the detection of deoxyribonucleic acid (DNA) of cytomegalovirus, Epstein Barr and herpes simplex 1 and 2 from biological samples.

Materials and Methods

We developed a rigorous systematic review of scientific literature on the subject during the years 2000 to 2009, using different databases. The inclusion and exclusion criteria defined for the articles were applied independently by two investigators and, finally, three studies were considered for data analysis. resultsThe findings show a simple, rapid method for detecting microorganism by LAMP test when compared with its predecessor PCR assay. In addition, the test has a good performance with a sensitivity of 80% to 86%, specificity of 85.7% to 100%, positive predictive value of 66.7% to 100% and negative predictive value of 90% to 99.4%.

Discussion

According to published studies where the LAMP assay was used, this test is a molecular diagnostic tool similar to real-time PCR in terms of sensitivity and specificity, as demonstrated also in the studies selected for this review. The assay was evaluated using clinical samples and the results indicated the suitability and simplicity of the test as a rapid, field diagnostic tool. There are still very few original studies on the usefulness of the LAMP assay, however, this review presents a contribution to the field and provides clarity on the real and valid applications of the LAMP test.

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Author Biographies

Olga L. Rincón Caballero, Pablo Tobon Uribe Hospital

Bacteriologist and Clinical Laboratorian, Molecular Diagnostic Laboratory, Pablo Tobón Uribe Hospital. Researcher at the Center for Epidemiology and Molecular Diagnosis of Infectious Diseases, EDIMEI.

Beatriz H. Aristizábal Bernal, Pablo Tobon Uribe Hospital

Molecular Diagnostic Laboratory Coordinator, Pablo Tobón Uribe Hospital. Researcher Medical and Experimental Mycology Unit, Corporation for Biological Research (CIB). Researcher at the Center for Epidemiology and Molecular Diagnosis of Infectious Diseases, EDIMEI.

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