Mutagenicidad y genotoxicidad en fracciones de PM2,5 del aire de Villa del Rosario, Colombia

Iván Meléndez-G.; Greila P. Quintero-S.; Alfonso Quijano-Parra

doi:10.17533/udea.acbi.331387

Autores/as

Iván Meléndez-G. Universidad de Pamplona
Greila P. Quintero-S. Universidad de Pamplona
Alfonso Quijano-Parra Universidad de Pamplona

DOI:

https://doi.org/10.17533/udea.acbi.331387

Palabras clave:

cepas TA98 y TA100, ensayo cometa, Salmonella typhimurium, PM2.5

Resumen

Las partículas atmosféricas constituyen uno de los contaminantes atmosféricos más importantes, debido a que contienen metales e hidrocarburos aromáticos policíclicos (HAP) que son compuestos con conocida actvida genotóxica, mutagénica y carcinogénica. Las particulas finas con diámetro menor a 2,5 μm (PM_2,5) producidas por la combustión de los vehículos diésel están implicadas en efectos sobre la salud humana. El propósito de esta investigación fue determinar la actividad mutagénica y genotóxica de la fracción respirable del PM_2,5, presente cerca de la avenida fronteriza de alto flujo vehicular en Villa de Rosario (Norte de Santander), la cual comunica los países de Colombia y Venezuela. El PM_2,5fue evaluado con filtros de cuarzo Pallflex. La actividad mutagénica y genotóxica de los extractos del PM_2,5fue determinada mediante la prueba Ames y el ensayo cometa. En la prueba de Ames se utilizaron las cepas TA98 y TA100 de Salmonella typhimurium y el daño genotóxico se evaluó en linfocitos de sangre periférica. Los resultados mostraron mutagenicidad y genotoxicidad asociada con la fracción PM_2,5en Villa del Rosario, lo cual representa un riesgo en la aparición de enfermedades como el cáncer en la población expuesta.

|Resumen

= 357 veces | PDF

= 106 veces| | HTML

= 165 veces|

Descargas

Los datos de descargas todavía no están disponibles.

Biografía del autor/a

Iván Meléndez-G., Universidad de Pamplona

Profesor, Departamento de Biología, Facultad de Ciencias Básicas, Grupo de Investigación en Biología Molecular (Biomogen), Universidad de Pamplona. Pamplona (Santander del Norte), Colombia.

Greila P. Quintero-S., Universidad de Pamplona

Departamento de Química, Facultad de Ciencias Básicas, Grupo de Investigación en Química, Universidad de Pamplona. Pamplona (Santander del Norte), Colombia.

Alfonso Quijano-Parra, Universidad de Pamplona

Profesor, Laboratorio de Control de Calidad, Departamento de Química, Facultad de Ciencias Básicas, Grupo de Investigación en Química, Universidad de Pamplona. Pamplona (Santander del Norte), Colombia.

Citas

Arlt VM. 2005. 3-Nitrobenzanthrone, a potential human cancer hazard in diesel exhaust and urban air pollution: a review of the evidence. Mutagenesis, 20 (6): 399-410. DOI: https://doi.org/10.1093/mutage/gei057

Boström CE, Gerde P, Hanberg A, Jernstrom B, Johansson C, Kyrklund T, Rannug A, Tornqvist M, Victorin K, Westerholm R. 2002. Cancer risk assessments, indicators, and guidelines for polycyclic aromatic hydrocarbons in the ambient air. Environmental Health Perspective, 110 (3): 451-488. DOI: https://doi.org/10.1289/ehp.110-1241197

Enya T, Suzuki H, Watanabe T, Hirayama T, Hisamatsu Y. 1997. 3 Nitrobenzanthrone, a powerful bacterial mutagen and suspected human carcinogen found in diesel exhaust and airborne particles. Environmental Science Technology, 31 (10): 2772-2776. DOI: https://doi.org/10.1021/es961067i

Greenwell LL, Moreno T, Jones TP, Richards RJ. 2002. Particle induced oxidative damage is ameliorated by pulmonary antioxidants. Free Radical Biology and Medicine, 1 (32): 898-905. DOI: https://doi.org/10.1016/S0891-5849(02)00782-7

Ianistcki M, Dallarosa J, Sauer C, Teixeira CE, da Silva J. 2009. Genotoxic effect of polycyclic aromatic hydrocarbons in the metropolitan area of Porto Alegre, Brazil, evaluated by Helix aspersa (Müller, 1774). Environmental Pollution, 157 (7): 2037-2042. DOI: https://doi.org/10.1016/j.envpol.2009.02.025

IARC (International Agency for Research on Cancer). 1984. Polynuclear aromatic compounds, Part 3, Industrial exposures in aluminium production, coal gasification, coke production, and iron and steel founding. Vol 34. Monographs on the evaluation of the carcinogenic risk of chemicals to humans. Lyon (France): World Health Organization International Agency for Research on Cancer. p. 222.

Janssen NAH, Fischer P, Marra M, Ameling C, Cassee FR. 2013. Short-term effects of PM2.5, PM10 and PM2.5-10 on daily mortality in The Netherlands. Science of The Total Environment, 463-464: 20-26. DOI: https://doi.org/10.1016/j.scitotenv.2013.05.062

Kaonga B, Kagabi NA. 2010. Investigation into presence of atmospheric particulate matter in Marikana, mining área in Rustenburg Town, South Africa. Environmental Monitoring and Assessment, 178 (1-4): 213-220. DOI: https://doi.org/10.1007/s10661-010-1683-1

Kawanaka Y, Matsumoto E, Wang N, Yun S-J, Sakamoto K. 2008. Contribution of nitrated polycyclic aromatic hydrocarbons to the mutagenicity of ultrafine particles in the roadside atmosphere. Atmospheric Environment, 42 (32): 7423-7428. DOI: https://doi.org/10.1016/j.atmosenv.2008.06.032

Künzli N, Perez L, Rapp R. 2010. Air Quality and Health. Lausanne (Switzerland): European Respiratory Society. World Health Organization. p. 89.

Klaunig JE, Kamendulis LM. 2004. The role of oxidative stress in carcinogenesis. Annual Review of Pharmacology and Toxicology, 44: 239-267. DOI: https://doi.org/10.1146/annurev.pharmtox.44.101802.121851

Li N, Xia T, Nel AE. 2008. The role of oxidative stress in ambient particulate matter induced lung diseases and its implications in the toxicity of engineered nanoparticles. Free Radical Biology and Medicine, 44 (9): 1689-1699. DOI: https://doi.org/10.1016/j.freeradbiomed.2008.01.028

Mehta S, Shin H, Burnett R, North T, Cohen AJ. 2013. Ambient particulate air pollution and acute lower respiratory infections: a sistematic review and implications for estimating the global burden of disease.

Air Quality, Atmosphere, and Health, 6 (1): 69-83. Meléndez-Gélvez I, Martínez-Montañez ML, Quijano-Parra A. 2012. Actividad mutagénica y genotóxica en el material particulado fracción respirable MP2,5 en Pamplona, Norte de Santander, Colombia. Iatreia, 25 (4): 347-356. DOI: https://doi.org/10.17533/udea.iatreia.13131

Mortelmans K, Zeiger E. 2000. The Ames Salmonella/microsome mutagenicity assay. Mutation Research, 455 (1-2): 29-60. DOI: https://doi.org/10.1016/S0027-5107(00)00064-6

Nel A. 2005. Atmosphere. Air pollution-related illness: effects of particles. Science, 308 (5723): 804-806. DOI: https://doi.org/10.1126/science.1108752

Novotna B, Topinka J, Solansky I, Chvatalova I, Lnenickova Z, Sram RJ. 2007. Impact of air pollution and genotype variability on DNA damage in Prague policemen. Toxicology Letters, 172 (1-2): 37-47. DOI: https://doi.org/10.1016/j.toxlet.2007.05.013

Pandrangi R, Petras M, Ralph S, Vrzoc M.1995. Alkaline single cell gel (comet) assay and genotoxicity monitoring using bulheads and carp. Environmental and Molecular Mutagenesis, 26 (4): 345-356. DOI: https://doi.org/10.1002/em.2850260411

Quijano-Parra A, Quijano-Vargas MJ, Meléndez-Gelvez I. 2015. Genotoxicidad de los contaminantes prioritarios en el aire de Villa del Rosario-Norte de Santander, Colombia. Revista Universidad y Salud, 17 (1): 69-79. DOI: https://doi.org/10.24054/01204211.v1.n1.2016.1929

Raaschou-Nielsen O, Andersen ZJ, Beelen R, Samoli E, Stafoggia M, Weinmayr G, Hoffmann B, Fischer P, Nieuwenhuijsen MJ Brunekreef B, Xun WW, Katsouyanni K, Dimakopoulou K, Sommar J, Forsberg B, Modig L, Oudin A, Oftedal B, Schwarze PE, Nafstad P, De Faire U, Pedersen NL, Ostenson CG, Fratiglioni L, Penell J, Korek M, Pershagen G, Eriksen KT, Sørensen M, Tjønneland A, Ellermann T, Eeftens M, Peeters PH, Meliefste K, Wang M, Bueno-de-Mesquita B, Key TJ, de Hoogh K, Concin H, Nagel G, Vilier A, Grioni S, Krogh V, Tsai MY, Ricceri F, Sacerdote C, Galassi C, Migliore E, Ranzi A, Cesaroni G, Badaloni C, Forastiere F, Tamayo I, Amiano P, Dorronsoro M, Trichopoulou A, Bamia C, Vineis P, Hoek G. 2013. Air pollution and lung cancer incidence in 17 European cohorts: prospective analyses from the European Study of Cohorts for Air Pollution Effects (ESCAPE). The Lancet Oncology, 14 (9): 813-822. DOI: https://doi.org/10.1016/S1470-2045(13)70279-1

Shah ASV, Langrish JP, Nair H, McAllister DA, Hunter AL, Donaldson K, Newby DE, Mills NL. 2013. Global association of air pollution and heart failure: a systematic review and meta-analysis. The Lancet, 382 (9897): 1039-1048. DOI: https://doi.org/10.1016/S0140-6736(13)60898-3

Sevastyanova O, Novakova Z, Hanzalova K, Binkova B, Sram RJ, Topinka J. 2008.Temporal variation in the genotoxic potential of urban air particulate matter. Mutation Research, 649 (1-2): 179-186. DOI: https://doi.org/10.1016/j.mrgentox.2007.09.010

Silva RA, West JJ, Zhang Y, Anenberg SC, Lamarque J-F, Shindell DT, Collins WJ, Dalsoren S, Faluvegi G, Folberth G, Horowitz LW, Nagashima T, Naik V, Rumbold S, Skeie R, Sudo K, Takemura T, Bergmann D, Cameron-Smith P, Cionni I, Doherty RM, Eyring V, Josse B, MacKenzie IA, Plummer D, Righi M, Stevenson DS, Strode S, Szopa S, Zeng G. 2013. Global premature mortality due to anthropogenic outdoor air pollution and the contribution of past climate change. Environmental Research Letters, 8: 034005 (11pp.). DOI: 10.1088/1748-9326/8/3/034005. DOI: https://doi.org/10.1088/1748-9326/8/3/034005

Slezakova K, Castro D, Begonha A, Delerue-Matos C, Alvim-Ferraz MdC, Morais S, Pereira MdC. 2011. Air pollution from traffic emissions in Oporto, Portugal: Health and environmental implications. Microchemical Journal, 99 (1): 51-59. DOI: https://doi.org/10.1016/j.microc.2011.03.010

Traversi D, Degan R, De-Marco R, Gilli G, Pignata C, Villani S, Bono R. 2009. Mutagenic properties of PM2.5 urban pollutionin Northern Italy: the nitro-compounds contribution. Environment International, 35 (6): 905-910. DOI: https://doi.org/10.1016/j.envint.2009.03.010

Topinka J, Rossner PJr, Milcova A, Schmuczerova J, Svecova V, Sram RJ. 2011. DNA adducts and oxidative DNA damage induced by organic extracts from PM2.5 in an acellular assay. Toxicology Letters, 202 (3): 186-192. DOI: https://doi.org/10.1016/j.toxlet.2011.02.005

U.S. EPA. 2004. Air quality criteria for particulate matter (Final Report, Oct 2004). Washington, D. C.: U.S. Environmental Protection Agency. EPA 600/P-99/002aF-bF, 2004.

WHO/IPCS (Kielhorn J, Wahnschaffe U, Mangelsdorf I). 2003. Selected nitro- and nitro-oxy-polycyclic aromatic hydrocarbons. Environmental Health Criteria, 229: 1-480 [Internet]. Geneva (Italy): United Nations Environment Programme, International Labour Organization, World Health Organization, Inter- Organization Programme for the Sound Management of Chemicals. Accessed: 3 May 2016. Available from: < http://apps. who.int/iris/bitstream/10665/42537/1/WHO_EHC_229.pdf>.

Yang XY, Igarashi K, Tang N, Lin JM, Wang W, Kameda T, Toriba A, Hayakawa K. 2010. Indirect- and directacting mutagenicity of diesel, coal and wood burning-derived particulates and contribution of polycyclic aromatic hydrocarbons and nitropolycyclic aromatic hydrocarbons. Mutation Research, 695 (1-2): 29-34. DOI: https://doi.org/10.1016/j.mrgentox.2009.10.010

Zhang Y, Yang B, Gan J, Liu C, Shu X, Shu J. 2011. Nitration of particle-associated PAHs and their derivatives (nitro-, oxy-, and hydroxy-PAHs) with NO3 radicals. Atmospheric Environment, 45 (15): 2515-2521. DOI: https://doi.org/10.1016/j.atmosenv.2011.02.034