Antibacterial activity, determination of total polyphenols by Folin-Ciocalteu and toxicity in Artemia salina of plant species Bauhinia variegata

Abstract

Phenolic compounds, which include flavonoids, possess pharmacological  properties  such  as  antibacterial, antioxidant, and vasodilating. The species of Bauhiniavariegata is  characterized  by  its  antidiabetic  and antibacterial action and it is important to correlate the contribution of the Polyphenolic constituents of the functional activity presented, in addition to determine its toxicity to Artemia salina. Preliminary phytochemical analysis was performed to confirm the presence of phenols. After confirming phenols and their abundance, Folin-Ciocalteu reagent was used to quantify phenols. The antibacterial activity was performed using the disk diffusion technique. A. salina nauplii were used to assess toxicity. Presence of saponins, phenolic compounds, was  observed  showing  different  colorations.  The quantification  of  total  polyphenols  showed  28,  5% mg equivalent of Gallic acid (EAG 100 g of extract polar). Against bacteria analysis presented inhibition halos. Found a CL50 of 1205 μg/mL front A. salina, indicating  that  the  summary  is  not  toxic.  Results indicate the phenolic compound potential of the plant and its possible use as an antibacterial agent.