Molecular detection of Escherichia coli pathotypes associated to acute diarrheal disease in children in a hospital in Rosario, Argentina
Escherichia coli pathotypes in children
DOI:
https://doi.org/10.17533/udea.hm.v14n1a03Keywords:
Diarrheal disease., Escherichia coli., Prevalence., Polimerase chain reaction., Pathotypes., Diagnostic.Abstract
Objective: Detect Escherichia coli pathotypes in a pediatric population with acute diarrheal disease in the city of Rosario to adopt timely and effective intervention measures in Public Health. Material and methods: A total of 360 fecal samples from pediatric patient with ADD and who attended in a pediatric hospital in Rosario, Argentina, were studied. The research was carried out from January 2021 to April 2022. The identification of pathotypes was carried out by polymerase chain reaction. Results: 21.11% diarrheagenic Escherichia coli (DEC) strains were identified. The virulence genes corresponding to all the pathotypes studied were detected: enteroaggregative E. coli (EAEC) (42.10%), enteroinvasive E. coli (EIEC) (23.68%), enterotoxigenic E. coli (ETEC) (14.47%), enteropathogenic E. coli (EPEC) (15.79%) and enterohemorrhagic E. coli (EHEC) (1.32%). Mixed EAEC/EPEC (1.32%) and ETEC/EAEC (1.32%) infections were detected. Conclusions: Molecular diagnostic methods are a fundamental tool in the study of the epidemiology of acute diarrheal disease. The application of these techniques is essential to identify the etiological agent of DEC, reduce the spread of these pathogens and thus reduce morbidity and mortality associated with diarrhea in the pediatric population.
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