Production of fungi slides for teaching
DOI:
https://doi.org/10.17533/udea.hm.332002Keywords:
teaching, fungi, slidesAbstract
Introduction: the aim of this research was to describe a method for producing fungi slides to be used in teaching.
Methods: a total of 420 agar blocks of 28 different species of fungi were produced and three dyes were evaluated: lactophenol blue, and plant-derived blue and red.
Results: the methodology allowed the visualization of intact conidiogenous structures in most fungi. After one year of storage, 96.1% of the 420 slides prepared were in good condition, both macroscopically and microscopically.
Conclusions: the implementation of fungi slides was considered an innovative methodology to facilitate the training process and optimize the way of teaching mycology.Keywords:teaching, fungi, slides.
Downloads
References
González MS, Diamont PD, Gutiérrez B. Técnica de tinción de estructuras fúngicas con colorantes vege-tales como una alternativa no contaminante. Bioagro. 2011;23:65-8.
Johnson EA. An Improved Slide Culture Technique for the Study and Identification of Pathogenic Fungi. J Bacteriol. 1946;51(6):689-94.
Koneman EW, Koneman AS. Diagnóstico microbio-lógico. Texto y atlas en color. Bogotá: Editorial Médica Panamericana; 2008.
Booth C. Methods in Microbiology. Londres: Elsevier Science; 1971.
Woo PC, Ngan AH, Chui HK, Lau SK, Yuen KY. Agar block smear preparation: a novel method of slide prep-aration for preservation of native fungal structures for microscopic examination and long-term storage. J Clin Microbiol. 2010;48(9):3053-61.
Larone DH. Medically Important Fungi: A Guide to Identification. Washington: American Society for Mi-crobiology; 2002.
Hughes AD, Lorusso GD, Greer DL. The ‘double-layer tape prep’: an improvement to a standard technique. J Med Microbiol. 2004;53(Pt 5):45.
Bonifaz A. Micología médica básica. 3.a ed. México: McGraw Hill; 2010.
Morán I. Toxicología clínica. España: Grupo Difusión; 2011.
Mora C, Berbeo M. Manual de Gestión Integral de Residuos. [Internet]. Colombia: Instituto Nacional de Salud; 2010. [Consultado 2018 Feb 05]. Disponible en: https://www.minsalud.gov.co/sites/rid/Lists/Bibliote-caDigital/RIDE/IA/INS/manual-gestion-integral-resi-duos.pdf11. Agency for Toxic Substances & Disease Registry. Toxicological profile for Phenol. [Internet]. 2008. [Con-sultado 2018 Feb 05]. Disponible en: http://www.atsdr.cdc.gov/ToxProfiles/tp.asp?id=148&tid=27
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 Hechos Microbiológicos

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.