Genotipificación de la región 3´UTR del gen Nramp1, en ganado Holstein y en criollo Hartón del Valle
DOI:
https://doi.org/10.17533/udea.rccp.324097Keywords:
atmospheric pressure, fermentation, food.evaluationAbstract
Resumen
En investigaciones sobre respuesta a infecciones por diferentes patógenos intracelulares en humanos y en ratones, el gen Nramp1 se ha descrito como un gen controlador de la resistencia y de la susceptibilidad a algunas enfermedades. En el presente trabajo se genotipificaron 151 bovinos criollos Hartón del Valle, y 128 Holstein, para un microsatélite de la región 3' UTR(que no transcribe), en la posición 1745-1955 del gen Nramp1. Se determinaron variaciones de tamaño para éste microsatélite en ambos grupos y se encontró que los más frecuentes son el de 209 y el de207 pares de bases. Los resultados de secuenciación mostraron modificaciones en el número de GTs y se encontraron los polimorfismos (GT)12, (GT)11, previamente reportados por Feng et al, y Horín et al; además se encontraron (GT)14 y (GT)10.
Summary
In studies of natural resistance or susceptibility to different intracellular pathogens the Nramp1 gene has been described as controlling this characteristic in mouse and in the humans. In this project we genotyped 151 Harton of the Valle bovine individuals and 128 Holstein, for a microsatellite of the 3' untranslated region of Nramp1 gene, in position 1745-1955 and found that the most frequency size of microsatellite were 207 and 211 bp. The sequencing results indicate that there is polymorphim in the number of GT repetitions and (GT)12 and (GT)11 were present as reported previously, as well as (GT)14 y (GT)10.
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References
Chang R. Química. 6 ed. México: McGraw-Hill;México: McGraw-Hill; 1999. 995 p
Fox DG, Sniffen CJ, O`Connor JD, Russell JB, Van Soest PJ. A net carbohydrate and protein system for evaluatingA net carbohydrate and protein system for evaluating cattle diets: III. Cattle requirements and diet adequacy. J Anim Sci 1992; 70:3578-3596 DOI: https://doi.org/10.2527/1992.70113578x
Goering HK, Van Soest PJ. Forage fiber analysis. Agric.oering HK, Van Soest PJ. Forage fiber analysis. Agric. Handbook No. 379. Washington: Agricultural Research Service-USDA; 1970.
Holdridge LR. Life zone ecology. San José de Costa Rica: Tropical Science center; 1967; 206 p.
López S, Carro MD, González JS, Ovejero FJ. ComparisonComparison of different in. vitro and in. situ methods to estimate the extent and rate of degradation of hays in the rumen. Anim Feed Sci and Technol 1998; 73: 99-113 DOI: https://doi.org/10.1016/S0377-8401(98)00129-1
Mauricio RM, Mould FL, Dhanoa MS, Owen E, Channa KS, et.al. A semi-automated in.vitro gas production technique for ruminants fedstuff evaluation. Anim Feed Sci Technol 1999; 79: 321-330 DOI: https://doi.org/10.1016/S0377-8401(99)00033-4
Mauricio RM, Owen E, Mould FL, Givens I, Theodorou MK, et.al. Comparison of bovine rumen liquor and bovine faeces as inoculum for an in.vitro gas production technique for evaluating forages. Anim Feed Sci Technol 2001; 89:33-48. DOI: https://doi.org/10.1016/S0377-8401(00)00234-0
Mauricio RM, Pereira LGR, Gonçalves LC, Rodríguez NM. Relação entre pressão e volume para implantação da técnica in.vitro semi-automática de produção de gases na avaliação de forrageiras tropicais. Arq Bras Med VetArq Bras Med Vet Zootec 2003; 55:216-219. DOI: https://doi.org/10.1590/S0102-09352003000200014
Menke KH, Raab L, Salewski A, Steingass H, Fritz D, et.al . The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in.vitro. J Agric Sci 1979; 93:217-222. DOI: https://doi.org/10.1017/S0021859600086305
Menke KH, Steingass H. Estimation of the energetic feed value obtained from chemical analysis and in . vitrogas production using rumen fluid. Anim Res Dev 1988; 28: 7-55.
National Research Council - NRC. Nutrient requirements of dairy cattle. 7 ed. Washington: National Academy Press; 2001. 381 p.
Noguera JR. Estudo químico, “Estudo químico, “in.situ”, “in.vitro” e microscópico da parede celular de cinco genótipos de sorgo colhidos em três epocas de corte. Ph. D. Thesis. Belo Horizonte: Escola de Veterinária da Universidade Federal de Minas Gerais, 2002. 148p.
Orskov ER, DeB Hovell FD, Mould F. Uso de la técnica de la bolsa de nylon para la evaluación de alimentos. Prod Anim Trop 1980; 5:213-223.
Pell AN, Doane PH, Schofield P. In vitro digestibility and gas production. In: Simpósio sobre Tópicos Especiais emIn: Simpósio sobre Tópicos Especiais em Zootecnia, Lavras, MG; 1997. 109 –132 p.
Pell AN, Schofield P. Computerized monitoring of gasPell AN, Schofield P. Computerized monitoring of gas production to measure forage digestion in.vitro. J Dairy Sci 1993; 76: 1063-1073 DOI: https://doi.org/10.3168/jds.S0022-0302(93)77435-4
Posada SL, Noguera RR. Técnicaosada SL, Noguera RR. Técnica in.vitro.de producción de gases: Una herramienta para la evaluación de alimentos para rumiantes. Livestock Research for RuralLivestock Research for Rural Development. 2005;.17 (36). URL:URL:http://www.cipav.org.co/lrrd/lrrd17/4/posa17036.htm
Russell JB, O`Connor JD, Fox DG, Van Soest PJ, Sniffen CJ. A net carbohydrate and protein system for evaluatingA net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. J Anim Sci 1992; 70:3551-3561. DOI: https://doi.org/10.2527/1992.70113551x
Sniffen CJ, O`Connor JD, Van Soest PJ, Fox DG, Russellniffen CJ, O`Connor JD, Van Soest PJ, Fox DG, Russell JB. A net carbohydrate and protein system for evaluatingA net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and Protein Availability. J Anim Sci 1992; 70: 3562-3577. DOI: https://doi.org/10.2527/1992.70113562x
Statistical Analysis Systems. SAStatistical Analysis Systems. SAS®, versión 8.2 para Windows,. User ́s Guide. Statistics. Statistical Analysis Systems Institute. Inc., Cary, North Carolina; 2001.
Theodorou MK, Williams BA, Dhanoa MS, McAllanheodorou MK, Williams BA, Dhanoa MS, McAllan AB, France J. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim Feed Sci and Technol 1994; 48:185-197. DOI: https://doi.org/10.1016/0377-8401(94)90171-6
Tilley JMA, Terry RA. A two stage technique for the in.vitro digestion of forage crops. J Br Grass Soc 1963; 18: 104-111.22. Umland J, Bellama J. Química General. 3rd ed. México: Thomson editores; 2001. 1017 p. DOI: https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
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