Estimación de polimorfismos del gen de leptina bovino en poblaciones de las razas criollas Hartón del Valle, Blanco Orejinegro (BON) y en la raza Brahman

Authors

  • María T Guerra
  • Esperanza Trujillo Bravo
  • Mario Fernando Cerón Muñoz

DOI:

https://doi.org/10.17533/udea.rccp.324015

Keywords:

microsatellite, obese gene, ST, WD

Abstract

Resumen

En investigaciones recientes se han identificado algunas regiones entre y adyacentes al gen de leptina, asociadas con diferentes niveles de grasa de la carne en canal, y cuyas frecuencias varían grandemente de una raza a otra, y de una población a otra. En este trabajo, fueron determinados los polimorfismos para la región 5 UTR´ (región que no traduce) y región flanqueante al extremo 3´ del gen leptina, utilizando dos microsatélites ST y WD (G18586 y U50365). Fueron evaluados 261 animales de las razas Hartón del Valle (100), Brahman (121) y BON (40). Se encontraron 7 alelos diferentes para el marcador ST y 15 para WD, considerando la población total analizada. Las poblaciones de las razas criollas evaluadas para los marcadores ST y WD, no se encuentran en equilibrio de HardyWeinberg.  El mayor polimorfismo para el microsatélites ST se encontró en la raza Brahman, que presentó 6 alelos. Para WD fue más polimórfica Hartón del Valle con 15 alelos diferentes. La menos polimórfica fue la raza BON, con 4 alelos para ST y 6 para WD.

Summary

Recent investigations have identified some regions between and adjacent to the leptin gene, associated with different carcass fat levels in bovines in wich frequencies vary greatly from one race to another, and from one population to another. This investigation determined the polymorphisms for the region 5 UTR´ and flanking region to the end 3' of the leptin gene using two markers ST and WD (G18586 and U50365). 261 animals were evaluated of Harton del Valle (100), Brahman (121) and BON (40) cattle. Were evaluated the result of this analysis where, 7 different alelles for microsatellite ST and 15 for WD, in the total population. The creole populations evaluated for marker ST are not in Hardy-Weinberg (HW) equilibrium. The marker WD only appeared H-W equilibrium for the population Brahman.  The Brahman cattle was the most polymorphic cattle for microsatellites ST, where 6 for ST alleles were found. For WD the most polymorphic cattle was Harton del Valle and less polymorphic cattle was the BON, with 6 for ST and 6 for WD alleles.

 

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References

Auwerx J. and Staels, B. 1998. Leptin. Lancet. 351: 737-742.

Buchanan FC, Fitzsimmons CJ, Van Kessel, AG, Thue TD,Windkelman-Sim DC, Schmutz SM. 2002. Association of amissense mutation in the bovine leptin gene with carcass fatcontent and leptin mRNA levels. Genet. Sci. Evol. 34: 105-116.

Coleman DL. 1978.Obese and diabetes: two mutant genescausing diabetes-obesity syndromes in mice. Diabetologia14: 141-148.

Fitzsimmons CJ, Schmutz SM, Bergen RD, and McKinnonJJ. 1998. A potential association between the BM1500microsatellite and fat deposition in beef cattle. Mamm.Genome 9: 432-434.

Funahashi T, Simomura I, Hiraoka H, Arai T, Takahashi M,Nakamura T, Nozaki S Yamashita S, Takemura K, MatsuzawaY, 1995. Enhanced expression of rat obese (OB) gene inadipose tissues of ventromedial hypothalamus (VMH)-lesioned rats. Biochem Biophys Res Commun. 211: 469-475.

Geary TW, McFadin EL, MacNeil MD, Grings EE, ShortRE, Funston RN, and Keisler DH. 2003. Leptin as apredictor of carcass composition in beef cattle. J.Anim. Sci.81: 1-8.

Hale CS, Herring WO, Johnson GS, Shibuya H, Lubahn, D.B. and Keisler, DH. 1998. Evaluation of the leptin gene as apossible marker of carcass traits in Angus cattle. UMCAnimalSciences Departmental Report, 25-27.

Houseknecht KL, Baile CA, Matteri RL, Spurlock ME. 1998.The Biology of Leptin: A Review. J. Anim. Sci. 76: 1405-1420.

Hossner KL. 1998. Cellular, molecular and physiologicalaspects of leptin: Potential application in animal production.Can. J. Anim. Sci. 78:463-472.

Ji S, Willis GM, Scott RR, Spurlock ME, 1998. Partial cloningand expression of the bovine leptin gene. Anim. Biotechnol.9: 1-14.

Margetic S, Gazzola C, Pegg GG, and Hill R A. 2002. Leptin:a review of its peripheral actions and interactions. Inter. J.Obes. 26: 1407-1433.

Marquess L. 2001. The leptin gene as a candidate for fatdeposition assessment. Mimeo.

Masuzaki H, Ogawa Y, Isse N, Satoh N, Okasaki TM,Shigemoto M, Mori K, Tamura N, Hosada K, Yoshimasa Y,Jingami H, Kawada T, and Nakao K. 1995. Human obeseexpression and regional differences in the adipose tissue.Diabetes. 44: 855-858

Miller SA, Dykes DD, Poletsky HF. 1988 A simple saltingout procedure for extracting DNA from human nucleatedcells. Nucl Acids Res 16: 1215.

Pomp D, Zou T, Clutter AC., and Barendse, W. 1997. Rapidcommunication: Mapping of leptin to bovine chromosome 4by linkage analysis of a PCR- based polymorphism. J. Anim.Sci. Vol. 75, p. 1427.

Raymond M, Rousset F. 1995.GENEPOP (VersiÛn 3.3):population genetics software for exact test and ecumenicism.J Heredity 86:248-249.

Stone RT, Kappes S. M. and Beattie C. (1997) Two polymorphicmicrosatellites within an 18 kb genomic clone containing thebovine ob gene. Animal Genetics. 27 (Suppl. 2), 64.

Tartaglia LA, Dembski M, Weng X, Deng N, Culpepper J,Devos R, Richards JG, Campfield LA, Clark FT, Deeds J,Muir C, Sanker S, Moriarty A, Moore KJ, Smutko JS, MaysGG, Woolf EA, Monroe CA, and Tepper RI. 1995.Identification and expression cloning of a leptin receptor,OB-R. Cell 83: 1263-1271.

Tessanne K, Hines HC, and Davis ME, 1999. Relationshipsof Polymorphisms in the Bovine Leptin Gene withDifferences in Beef Carcass Traits. Research and Reviews:Beef and Sheep. Special Circular.170.

Wegner J, Huff P, Xie CP, Schneider F, Teuscher F, Mire PS,Mir Z, Kazala, EC, Weselake RJ and Ender K. 2001.Relationship of plasma leptin concentration to intramuscularfat content in beef from crossbred Wagyu cattle. Can. J.Anim. Sci. 81:451-457.

Wheeler TL, Cundiff LV and Koch RM. 1994. Effect ofmarbling degree on beef palatability in Bos taurus and Bosindicus cattle. J. Anim. Sci. 72:3145-3151.

Wilkins RJ and Davey H. 1997.A polymorphic microsatellitein the bovine leptin gene. Animal Genetics 38: 370-383.

Zhang Y, Proenca R, Maffel M, Barone M, Leopoldo L andFriedman JM. 1994.Positional Cloning of the mouse obesegene and its human homologue. Nature (Lond.) 372: 425-432.

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Published

2016-07-21

How to Cite

Guerra, M. T., Trujillo Bravo, E., & Cerón Muñoz, M. F. (2016). Estimación de polimorfismos del gen de leptina bovino en poblaciones de las razas criollas Hartón del Valle, Blanco Orejinegro (BON) y en la raza Brahman. Revista Colombiana De Ciencias Pecuarias, 18(3), 7. https://doi.org/10.17533/udea.rccp.324015

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