In vitro evaluation of probiotic potential of lactic bacteria acid isolated from coastal serum
DOI:
https://doi.org/10.17533/udea.acbi.13809Keywords:
acid pH and bile salt resistance, coastal serum, lactic acid, lactic acid bacteria, probioticAbstract
A group of 53 lactic acid bacteria (LAB) isolated from costal serum were evaluated with preliminary in vitro studies simulating the conditions of the digestive tract, to determine their characteristics as potential probiotics; acid pH tolerance (MRS pH: 2.0) and bile salts (MRS with 0.3% bile salts) and subsequently the surviving population was determined as log CFU/ml. Strains tolerant to such conditions were evaluated for resistance to 14 antibiotics of commercial use; we assessed adherence to intestinal mucus and the production of lactic acid by high performance liquid chromatography (HPLC). It was shown that 54.7% of the LAB evaluated were tolerant to acid pH conditions and 49.1 to 0.3% bile salts with a population of 106 log CFU/ml on average. Seven strains were selected by antibiotic vancomycin tenderness of epidemiological importance and adhered to intestinal mucus, meeting the requirements to be considered as potentially probiotic. Additionally, the quantification of lactic acid in the selected strains showed a range between 0.13 and 1.0 ± 0.05 ± 0.08 g/l.
Downloads
References
Adesokan Y, Odetoyinbo B, Okanlawon B. 2009. Optimization of lactic acid production by lactic acid bacteria isolater from some traditional fermented food in Nigeria. Pakistan Journal of Nutrition, 8 (5): 611-615.
Bao Y, Zhang Y, Zhang Y, Liu Y, Wanga Y, Dong X, Wang Y, Zhang H. 2010. Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products. Food Control, 21 (5): 695-712.
Canžek A, Bogovic B. 2001. Antibiotics influence on lactic acid bacteria inhabiting gastrointestinal tract. Mljekarstvo, 51 (2): 119-134.
Cebeci A, Gürakan C. 2003. Properties of potential probiotic Lactobacillus plantarum strains. Food Microbiology, 20 (5): 511-518.
Chou L, Weimer B. 1999. Isolation and characterization of acid- and bile-tolerant isolates from strains of Lactobacillus acidophilus. Journal of Dairy Science, 82 (1): 23-31.
Conway PL, Gorbach SL, Goldin BR. 1987. Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. Journal of Dairy Science, 70 (1): 1-12.
Cueto C, García D, Garcés F, Cruz J. 2007. Preliminary studies on the microbiological characterization of lactic acid bacteria in suero costeño, a Colombian traditional fermented milk product. Revista Latinoamericana de Microbiología, 49 (1-2): 12-18.
Dubey U, Mistry V. 1996. Growth characteristics of bifidobacteria in infant formulas. Journal of Dairy Science, 79 (7): 1146-1155.
Erkkilä S, Petäjä E. 2000. Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use. Meat Science, 55 (3): 297-300.
Gilliland S, Staley T, Bush L. 1984. Importance of bile toler-ance of Lactobacillus acidophilus used as dietary adjunct. Journal of Dairy Science, 67 (12): 3045-3051.
Kaufmann D, Fairchild K. 2004. Clinical microbiology of bacterial and fungal sepsis in very-low-birth-weight infants. Clinical Microbiology, 17 (3): 638-680.
Macfarlane S, Dillon JF. 2007. Review, microbial biofilms in the human gastrointestinal tract. Journal of Applied Microbiology, 102 (5): 1177-1436.
Maragkoudakis A, Zoumpopouloua G, Miarisa C, Kalantzopoulosa G, Potb B, Tsakalidoua F. 2006. Probiotic potential of Lactobacillus strains isolated from dairy products. International Dairy Journal, 16
(3): 189-199.
Marciňáková M, Klingberg T, Lauková T, Budde B. 2010. The effect of pH, bile and calcium on the adhesion ability of probiotic enterococci of animal origin to the porcine jejunal epithelial cell line IPEC-J2. Anaerobe, 16 (2): 120-124.
Mathur S, Singh R. 2005. Antibiotic resistance in food lactic acid bacteria —a review. International Journal of Food Microbiology, 105 (3): 281-295.
Naoko KM, Kenji O. 1996. Gastrointestinal colonization by methicillin-resistant Staphylococcus aureus in immunosuppressed mice. Infection and Immunity, 64 (10): 4231-4235.
Noriega L, Gueimonde M, Sánchez B, Margolles A, de los Reyes-Gavilán CG. 2004. Effect of the adaptation to high bile salts concentrations on glucosydic activity, survival at low pH and cross-resistance to bile salts in Bifidobacterium. International Journal of Food Microbiology, 94 (1): 79-86.
Ouwehand A, Suomalainen T, Tölkkö S, Salminen S. 2002. In vitro adhesion of propionic acid bacteria to human intestinal mucus. Lait 82 (1): 123-130.
Park S, Hwang Y, Kim Y, KIM J, Song J, Lee K, Jeong K, Rhee M, Kim K, Kim T. 2006. Comparison of pH and bile resistance of Lactobacillus acidophilus strains isolated from rat, pig, chicken, and human sources. Journal of Microbiology and Biotechnology, 22 (1): 35-37.
Pultz N, Vesterlund S, Ouwehand AC, Donskey CJ. 2006. Adhesion of vancomycin-resistant Enterococcus to human intestinal mucus. Current Microbiology, 52 (3): 221-224.
Ray A, Pultz N, Bhalla A, Aron D, Donskey C. 2003. Coexistence of vancomycin-resistant enterococci and Staphylococcus aureus in the intestinal tracts of hospitalized patients. Clinical Infectious Diseases, 37 (7): 875-881.
Savadogo A, Ouattara C, Bassole I, Traore SA. 2006. Bacteriocins and lactic acid bacteria —minireview. African Journal of Biotechnology, 5 (9): 678-683.
Shah N. 2000. Probiotic bacteria: selective enumeration and survival in dairy foods. Journal of Dairy Science, 83 (4): 894-907.
Sherr B, Sherr E, Fallon R. 1987. Use of monodispersed, fluorescently labeled bacteria to estimate in situ protozoan bacterivory. Applied and Environmental Microbiology, 53 (5): 958-965.
Strompfová V, Lauková A. 2004. Antibiotic resistance of acid lactic bacteria from canine faeces. Bulletin of Veterinary Institute in Pulawy, 48: 215-218.
Strompfová V, Lauková A, Ouwehand A. 2004. Selection of enterococci for potential canine probiotic additives. Veterinary Microbiology, 100 (1-2): 107-114.
WHO (World Health Organization) [Internet]. 2002. Guidelines for the evaluation of probiotics in food. Report of a joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. Food and Agriculture Organization. Fecha de acceso: 17 de diciembre de 2010. Disponible en: <http://www.who.int/foodsafety/
fs_management/en/probiotic_guidelines.pdf>.
Downloads
Published
How to Cite
Issue
Section
License
The authors exclusively authorize the Actualidades Biológicas journal to edit and publish the submitted manuscript if its publication is recommended and accepted, without this representing any cost to the Journal or the University of Antioquia.
All the ideas and opinions contained in the articles are sole responsibility of the authors. The total content of the issues or supplements of the journal is protected under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, so they cannot be used for commercial purposes, but for educational purposes. However, please mention the Actualidades Biológicas journal as a source and send a copy of the publication in which the content was reproduced.
