Study of Hinfi polymorphisms of the Pit-1 gene and their associations with type traits, milk yield and days open in Holstein cows from Antioquia, Colombia
DOI:
https://doi.org/10.17533/udea.acbi.13810Keywords:
animal breeding, Colombia, Holstein cows, molecular marker, Pit-1Abstract
The aim of this study was to determine allele and phenotypic frequencies for single nucleotide polymorphism (SNP) of exon VI in the Pit-1 gene and their associations with type traits, milk yield, and days open. A total of 390 Holstein cows from Antioquia (Colombia) were genotyped for the Pit-1 Hinfi polymorphism by PCR-RFLP. We found AA, AB, and BB genotypes with a frequency of 0.03, 0.43, and 0.53, respectively. The A allele frequency was 0.35 and its presence in the genotype was associated with higher milk production, udder depth and body depth; the absence of the A allele in the genotype was associated with fewer days open. This study indicates that it is possible to develop breeding programs using the Pit-1 gene in Holstein cattle in the department of Antioquia.
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References
Bauman DE, Eppard PJ, DeGeeter MJ, Lanza OM. 1985. Responses of high producing dairy cows to long-term treatment with pituitary somatotropin and recombinant somatotropin. Journal of Dairy Science, 68: 1352-1362.
Bech AM, Kristiansen RK. 1990. Milk protein polymorphism in Danish dairy cattle and the influence of genetic variants on milk yield. Journal Dairy Research, 57: 53-62.
Berry DP, Buckley F, Dillon P, Evans RD and Veerkamp RF. 2004. Genetic relationships among linear type traits, milk yield, body weight, fertility and somatic cell count in primiparous dairy cows. Irish Journal of Agricultural and Food Research, 43: 161-176.
De Mattos KK, Del Lama SN, Martínez ML, Freitas AF. 2004. Association of bGH and Pit-1 gene variants with milk production traits in dairy gyr bulls. Brazilian Journal of Agricultural Research, 39: 147-150.
Dybus A, Szatkowska I, Czerniawska-Platkowska E, Grzesiak W, Wojcik J, Rzewucka E and Zych S. 2004. Pit-1 Hinfi gene polymorphism and its associations with milk production traits in polish Black and White cattle. Archives Animal Breeding, 47: 557-563.
Edriss V, Edriss MA, Rahmani HR and Sayed-Tabatabaei BE. 2008. Pit-1 gene polymorphism of Holstein cows in Isfahan Province. Biotechnology, 7 (2): 209-212.
Hoggard N, Callaghan K, Levy A, Davis JRE. 1993. Expression of Pit-1 and related proteins in diverse human pituitary adenomas. Journal of Molecular Endocrinology, 11: 283-290.
Hori S, Barreras A. 2003. Relationships between DGAT1 and Pit-1 genes polymorphism and milk yield in Holstein cattle. Journal of Animal Science, 81 (Suppl. 1): 252
Jolanta O, Flisikowski K, Zwierzchowski L, Dymnicki E. 2003. Polymorphisms at loci of leptin of leptin (Lep), Pit-1 and STA-TSA and their association with growth, feed conversion and carcass quality in Black and White bulls. Animal Science Papers and Reports, 21: 135-145.
Lucy MC, Weber WJ, Baumgard LH, Seguin BS, Koenigsfeld AT, Hansen LB, Chester-Jones H, Crooker BA. 1998. Reproductive endocrinology of lactating dairy cows selected for increased milk production. Journal of Animal Science, 76 (Suppl. 1): 296.
McCormick A, Brady H, Theill LE, Kaim M. 1990. Regulation of the pituitary specific homeobox gene GHFl by cellautonomous and environmental cues. Nature, 345: 829-832.
Miller SA, Dykes DD, Poletsky HF. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16 (3): 1215.
Raymond M, Rousset F. 1995. GENEPOP (Versión 3.3) population genetics software for exact test and ecumenisism. Journal of Heredity, 86: 248-249.
Renaville R, Gengler N, Vrech E, Prandi S, Massart S, Corradini C, Bertozzi C, Mortiaux F, Burny A, Portelle D. 1997. Pit-1 gene polymorphism, milk yield and conformation traits for Italian Holstein Friesian bulls. Journal of Dairy Science, 80: 3431-3438.
SAS (Statistical Analysis Systems). 2006. Statistical Analysis Systems. SAS® (versión 9.1) para Windows. User’s Guide. Cary (North Carolina): Statistical Analysis Systems Institute. Inc. p. 846.
Sorensen P, Grochowska R, Holm L, Henryon M, Lovendahl P. 2002. Polymorphism in the bovine growth hormone gene affects endocrine release in dairy calves. Journal of Dairy Science, 85: 1887-1893.
Stanton TL, Blake RW, Quaas RL, Van Vleck LD. 1991. Response to selection of United States Holstein sires in Latin America. Journal of Dairy Science, 74: 651-664.
Steinfelder HJ, Hauser P, Nakayama Y, Radovick S, McClaskey JH, Taylor T, Weintraub BD, Wondisford FE. 1991. Thyrotropin-releasing hormone regulation of human TSHb expression: Role of a pituitary-
specific transcription factor (Pit-1/GHF-1) and potential interaction with a thyroid hormone- inhibitory element. Proceedings of the National Academy of Sciences, 88: 3130-3134.
Udina IG, Turkova SO, Kostyuchenko MV, Lebedeva LA, Sulimova GE. 2001. Polymorphism of bovine prolactin gene: microsatellites, PCR-RFLP. Russian Journal of Genetics, 37: 407-411.
Vargas LD, Gana V, Escudero I. 2004. Polimorfismo del gen Pit-1 en vacas lecheras de Chile central. Archivos de Zootecnia, 53: 217-220.
Veerkamp RF, Brotherstone S. 1997. Genetic correlations between linear type traits, food intake, live weight and condition score in Holstein Friesian dairy cattle. Animal Science, 64: 385-392.
Woollard J, Schmith CB, Freeman AE, Tuggle CK. 1994. Rapid comunication: Hinfi polimorphism at the bovine Pit-1 locus. Journal of Animal Science, 72: 3267.
Woollard J, Tuggle CK, Ponce de Leon FA. 2000. Rapid communication: localization of POU1F1 to bovine, ovine, and caprine 1q21-22. Journal of Animal Science, 78: 242-243.
Zwierzchowski L, Krzyzewski J, Strzalkowska N, Siadkowska E, Ryniewicz A. 2002. Effect of polymorphisms of growth hormone (GH), Pit-1, and leptin (LEP) genes, cow’s age, lactation stage and somatic cell count on milk yield and composition of Polish Black-and-White cows. Animal Science Papers and Reports, 20: 213-227.
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