Determination of the ability to degradate persistent organic compounds by marine sediment bacteria isolated in the Colombian Caribbean
DOI:
https://doi.org/10.17533/udea.acbi.329393Keywords:
biodegradation, chlorinate pesticides, hydrocarbons, marine bacteria, Bacillus, PseudomonasAbstract
82 native marine bacterial strains capable to tolerate persistent organic compounds under aerobic conditions were isolated from sediment samples from the Colombian Caribbean. Sixty-four (64) of these bacterial strains survived in laboratory conditions, 43 were isolated on medium enriched with organochlorinated pesticides (DDT and aldrin) and 21 strains from medium enriched with hydrocarbons (crude oil and diesel fuel). The 64 bacterial strains underwent a selection process with the purpose of choosing competitive bacteria to constitute mixed bacterial cultures and evaluate their ability to degrade hydrocarbons (Diesel fuel) and organochlorinated pesticides (DDT). Outcomes show that mixed bacterial culture grown on medium with hydrocarbons (CHM) was able to degrade in 68.61% of the n-alkanes fraction in the diesel fuel after 21 days. Bacterial culture grown on medium with pesticides (CMO), transformed a 68.8% DDT to DDD in 60 days. A 23.54% of the aliphatic hydrocarbon degradation and 29.1% of DDT, were due to abiotic factors. The strains were identified by the amplification of the gene 16s rRNA, finding in the CMO bacteria of genera Acinetobacter, Brevibacillus, and Pseudomonas, and the CMH microorganisms corresponding to the genera Bacillus, Enterobacteriaceae, Klebsiella, Pseudomonas, and Ralstonia. Strains examined had potential in the degradation of COP and they can be used like a strategic tool of bioremediation in the future.
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