Clorophyllase during baby banana (Musa accuminata) (Simons) ripening
DOI:
https://doi.org/10.17533/udea.acbi.329419Keywords:
baby banana, chlorophyll, chlorophyllase, Musa accuminataAbstract
The chlorophyllase (Chlase) of baby banana's peel (Musa accuminata) was extracted, partiality purified and characterized using as substrate chlorophyll (Chl-a) extracted from peel of the fruit. The activity of the Chlase was compared with respiratory intensity and ethylene production during storage of the baby banana at 20 °C.The characterization of enzyme showed follow optimal conditions: pH 7.0, 40 °C, KM 0.034 μM Chl-a, and VMAX 364 μM Chl-a hidrolizaded/min/mg protein. The storage test of the fruit showed both a climacteric maximum and Chlase maximum after 11 days. In this moment, the green color of the peel disappeared and permitted to see yellow color. This research permits to propose a direct relation between Chlase activity and less of Chl.
Downloads
References
AOAC. 1990. Official Methods of Analysis. Association ofOfficial Analytical Chemists, Inc. Washington D. C.,E. U. A.
Bustos C, Coy D. 1995. Estudio preliminar de las bajastemperaturas en el almacenamiento del banano bo-cadillo (Musa paradisiaca L.). Trabajo de Grado.Departamento de Farmacia, Universidad Nacional deColombia. Bogot·, Colombia.
DÌaz R, Porras G. 1998. DeterminaciÛn de la temperaturacrÌtica de almacenamiento del banano bocadillo(Musa paradisiaca L.). Trabajo de Grado. Departa-mento de QuÌmica, Universidad Nacional de Colom-bia. Bogot·, Colombia.
Dixon M, Webb EC. 1964. Enzymes. Academia Press. Nue-va York, E. U. A.
Funamoto Y, Yamauchi N, Shigenaga T, Sigyo M. 2002.Effects of heat treatment on chlorophyll degradingenzymes in stored broccoli (Brassica oleracea L.).Postharvest Biology and Technology, 24:163-170.
Guevara JC, Yahia EM, Brito de la FE, Biserka SP. 2003.Effects of elevated concentrations of CO2 in modifiedatmosphere packaging on the quality of prickly pearcactus stems (Opuntia spp.). Postharvest Biologyand Technology, 29:167-176.
Hornero-MÈndez D, MÌnguez-Mosquera MI. 2002.Chlorophyll disappearance and chlorophyllaseactivity during ripening of Capsicum annuum L.fruits. Journal of Science and Food Agricultural,82:1564-1570.
Iriyama K, Ogura N, Takamiya A. 1974. A simple methodfor extraction and partial purification of chlorophyllfrom plant material, using dioxane. Journal ofBiochemistry, 76:901-904.
Jacob-Wilk D, Holland D, Goldschmidt EE, Riov J, Eyal Y.1999. Chlorophyll breakdown by chlorophyllase:isolation and functional expression of the Chlase1gene from ethylene-treated citrus fruit and itsregulation during development. The Plant Journal,20:653-661.
Klein AO, Vishniac W. 1961. Activity and partialpurification of chlorophyllase in aqueous system.Journal of Biology and Chemistry, 236:2544-2549.
Monreal M, De Ancos B, Cano MP. 1999. Influence of criticalstorage temperatures on degradative pathways ofpigments in green beans (Phaseolus vulgaris cvs.Perona and Boby). Journal of Agricultural and FoodChemistry, 47:19-24.
Pardo Y. 2002. Efecto de dos inhibidores comerciales deetileno sobre la maduraciÛn de banano bocadillo(Musa accuminata) almacenado a diferentestemperaturas. Trabajo de Grado. Departamento deQuÌmica, Universidad Nacional de Colombia. Bogot·,Colombia.
Rubio ME. 1999. Estudio del cambio de actividad depolifenoloxidasa, PFO, durante el proceso de madu-raciÛn del lulo (Solanum quitoense L.). Trabajo deGrado Magister en Ciencias-QuÌmica, Departamentode QuÌmica, Facultad de Ciencias, Universidad Na-cional de Colombia. Bogot·, Colombia.
Salisbury BF, Ross CW. 1994. FisiologÌa vegetal.Grupo Editorial Iberoamericano. MÈxico D. F.,MÈxico.Su·rez C, Ariza S. 1996. Influencia de los choques de CO2sobre los daÒos por frÌo en el banano bocadillo (Musaaccuminata). Trabajo de Grado. Departamento deFarmacia, Universidad Nacional de Colombia. Bogo-t·, Colombia.
Serrato J. 1999. Estudio de la conservaciÛn de bananobocadillo mediante la aplicaciÛn combinada de cho-ques tÈrmicos y refrigeraciÛn. Trabajo de Grado. De-partamento de Farmacia, Universidad Nacional deColombia. Bogot·, Colombia.
Thomas P, Janave MT. 1992. Effect of temperature onchlorophyllase activity, chlorophyll degradation andcarotenoids of Cavendish banana during ripening.International Journal of Food Science andTechnology, 27:57-63.
Trebitsht T, Goldschmidt EE, Riov J. 1993. Ethylene indu-ces de novo synthesis of chlorophyllase, achlorophyll degrading enzyme, in citrus fruit peel.Proceedings of the National Academy of SciencesUSA, 90:9441-9445.
Wang YC. 1990. Chilling injury of horticulturalcrops. CRCPress, Inc. Florida, E. U. A.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Actualidades Biológicas
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The authors exclusively authorize the Actualidades Biológicas journal to edit and publish the submitted manuscript if its publication is recommended and accepted, without this representing any cost to the Journal or the University of Antioquia.
All the ideas and opinions contained in the articles are sole responsibility of the authors. The total content of the issues or supplements of the journal is protected under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, so they cannot be used for commercial purposes, but for educational purposes. However, please mention the Actualidades Biológicas journal as a source and send a copy of the publication in which the content was reproduced.
