confirmación molecular de especie en especímenes Anopheles recolectados en el corregimiento de Juan José,Puerto Libertador, Córdoba, colombia

Juliana Sánchez; Yadira Galeano; Doris A. Rosero; Nelson J. Naranjo; Margarita M. Correa

doi:10.17533/udea.hm.10875

Authors

Juliana Sánchez University of Antioquia
Yadira Galeano University of Antioquia
Doris A. Rosero University of Antioquia
Nelson J. Naranjo University of Antioquia
Margarita M. Correa University of Antioquia

DOI:

https://doi.org/10.17533/udea.hm.10875

Keywords:

Anopheles, Colombia, disease vectors , malaria, polymerase chain reaction, restriction fragment length polymorphism

Abstract

Introduction

Difficulty in the morphological identification of females of various species on the Nyssorhynchus subgenus has been documented; it is due to interspecies morphological similarity, intraspecific variation and the existence of cryptic species. For this reason, molecular techniques such as PCR-RFLP- ITS2 has been used, they allow confirming species assignation in endemic areas.

ObJective

To confirm the species assignation of mosquitoes Anopheles collected in Juan Jose locality, municipality

of Puerto Libertador, Cordoba, as a contribution to a species inventory in the locality.

Materials and methods

Of 2.070 specimens identified by morphological characters, 513 (24.8%) were molecularly confirmed

by PCR-RFLP-ITS2.

Results

The species defined by morphology as present in Juan José were: Anopheles (Nyssorhynchus) nuneztovari s.l. Gabaldon (97.44%), A. (Nys.) darlingi Root (0.77%) y A. (Nys.) oswaldoi s.l. Peryassu (0.10%). The rest of specimens (1.69%) were defined as belonging to one of the species of the Oswaldoi group. Molecular confirmation helped to determine the presence of only two species among the analyzed specimens, 99.23% A. (Nys.) nuneztovari and 0.77% A. (Nys.) darlingi.

Conclusions

The two species of Anopheles found in Juan Jose, during the sampling period are considered primary vectors in Colombia; this information is important to guide vector control efforts. Additionally, the results reaffirm previous reports on the difficulty of identifying A. nuneztovari s.l. by morphology and the importance of using molecular tests for species confirmation.

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Author Biographies

Juliana Sánchez, University of Antioquia

Student of Microbiology and Bioanalysis, Molecular Microbiology. Group, School of Microbiology, University of Antioquia. Medellín, Colombia.

Yadira Galeano, University of Antioquia

Student of Microbiology and Bioanalysis, Molecular Microbiology Group, School of Microbiology, University of Antioquia, Medellín, Colombia.

Doris A. Rosero, University of Antioquia

Microbiologist and Bioanalyst, Master's Student in Biology, Molecular Microbiology Group, School of Microbiology, Universidad de Antioquia, Medellín, Colombia.

Nelson J. Naranjo, University of Antioquia

Biologist, PhD Student in Biology, Molecular Microbiology Group, School of Microbiology, University of Antioquia, Medellín, Colombia.

Margarita M. Correa, University of Antioquia

Ph.D. in Microbiology, Professor, Molecular Microbiology Group, School of Microbiology,
University of Antioquia, Medellin, Colombia.

References

World Health Organization. Malaria, Fact sheet N°94, 2009. [Actualizado en enero de 2009 y revisado en abril de 2009] Disponible en: http://www.who.int/mediacentre/factsheets/fs094/en/index.html.

Global malaria Programme, World Health Organization. World Malaria Report. Geneva (Suiza), World Health Organization; 2008. 82 p.

Mantilla g, Oliveros H, barnston ag. The role of ENSO in understanding changes in Colombia’s annual malaria burden by region, 1960-2006. Malar J. 2009; 8: 6.

Instituto nacional de Salud. Subdirección de Vigilancia y Control en Salud Pública, Sistema de Vigilancia en Salud Pública – SIVIGILA. 2010. Semana Epidemiológica 52.

Gobernación de córdoba. Situación Epidemiológica del Programa de Enfermedades Transmitidas por Vectores. Secretaría de Desarrollo de la Salud. Montería, Córdoba, Colombia. 2008; 19 p.

González R. Globalizar la información de Colombia sobre Anopheles: necesidad de actualizar la información. Biomédica 2009; 29(Supl.): 135-7.

Olano V, brochero H, Saenz R, Quiñones m, Molina J. Mapas preliminares de la distribución de especies de Anopheles vectores de malaria en Colombia. Biomédica 2001; 21: 402-8.

Gutiérrez La, naranjo n, Jaramillo Lm, muskus c, Luckhart S, conn Je, correa mm. Natural infectivity of Anopheles species from the Pacific and Atlantic Regions of Colombia. Acta Trop. 2008; 107(2): 99-105.

Quiñones ML, Herrera m, Orjuela LI. Incriminación de vectores de malaria e implicaciones para su control. Biomédica. 2009; 29(Supl.): 116-8.

Quiñones ML, Ruiz F, calle da, Harbach Re, erazo HF, Linton Ym. Incrimination of Anopheles (Nyssorhynchus) rangeli and An. (Nys.) oswaldoi as natural vectors of Plasmodium vivax in Southern Colombia. Mem Inst Oswaldo Cruz. 2006; 101(6): 617-23.

Calle Ld, Quinones mL, erazo HF, Jaramillo On. Morphometric discrimination of females of five species of Anopheles of the subgenus Nyssorhynchusfrom Southern and Northwest Colombia. Mem Inst Oswaldo Cruz. 2002; 97(8): 1191-5.

Fernández R, Schoeler gb, Stancil J. Presencia deAnopheles benarrochien áreas de selva con transmisión malárica. Rev Perú Med Exp Salud Pública. 2004; 21: 217-22.

González R, Carrejo n. Introducción al estudio taxonómico de Anopheles de Colombia. Claves y notas de distribución. 2ª ed. Cali: Programa Editorial Universidad del Valle; 2009. 260p.

Gómez GF, Cienfuegos AV, gutiérrez LM, conn Je, correa mm. Análisis morfológico y molecular evidencia problemas al identificar Anopheles nuneztovari(Diptera: Culicidae) por claves dicotómicas. Rev Colombiana de Entomol. 2010; 36(1): 68-75.

Estrada da, Quiñones ML, Sierra Dm, Calle da, Ruiz F, erazo HF, Linton Ym. [Egg morphology as an indirect method to identify Anopheles benarrochi, Anopheles oswaldoi and Anopheles rangeli (Diptera: Culicidae)]. Biomédica. 2003; 23(4): 388-95.

Motoki MT, dos Santos CL S, Sallum ma. Intraespecific Variation on the Aedeagus of Anopheles oswaldoi (Peryassú) (Dipteria: Culicidae). Neotrop Entomol. 2009; 38(1): 144-8.

Calle da, Quiñones ML, Erazo HF, Jaramillo n. Discriminación por morfometría geométrica de once especies de Anopheles (Nyssorhynchus) presentes en Colombia. Biomédica. 2008; 28(3): 371-85.

Li C, Wilkerson R. Identification of Anopheles(Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes. Mem Inst Oswaldo Cruz. 2005; 100(5): 495-500.

Walton C, Somboon P, O’Loughlin Sm, Zhang S, Harbach Re, Linton Ym, chen b, nolan K, duong S, Fong mY, Vythilingum L, mohammed Zd, Ho dinh trung, butlin RK. Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA. Infect Genet Evol. 2007; 7(1): 93-102.

Brochero HH, Li c, Wilkerson Rc. A newly recognized species in the Anopheles (Nyssorhynchus) albitarsiscomplex (Diptera: Culicidae) from Puerto Carreno, Colombia. Am J Trop Med Hyg. 2007; 76(6): 1.113-7.

Zapata Ma, Cienfuegos aV, Quiros OI, Quinones ML, Luckhart S, Correa mm. Discrimination of seven Anopheles species from San Pedro de Urabá, Antioquia, Colombia, by polymerase chain reaction-restriction fragment length polymorphism analysis of its sequences. Am J Trop Med Hyg. 2007; 77(1): 67-72.

Cienfuegos AV, gómez GF, córdoba LA, Luckhart S, Conn J, Correa MM. Diseño y evaluación de metodologías basadas en PCR-RFLP de ITS2 para la identificación molecular de mosquitos Anopheles spp. (Diptera: Culicidae) de la Costa Pacífica de Colombia. Rev Biomed (Méx.). 2008; 19(1): 35-44.

Matson R, Tong Rios C, Banda Chavez c, Gilman RH, Florin D, Lopez Sifuentes V, Cardenas Greffa R, Fernandez R, Yori P, Velasquez portocarrrero d, Vinetz J, Kosec m. Improved molecular technique for the differentiation of neotropical anopheline species. Am J Trop Med Hyg. 2008; 78(3): 492-98.

Coleman AW. ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Trends Genet. 2003; 19(7): 370-5.

Marrelli mt, Floeter-Winter Lm, malafronte RS, tadei WP, Lourenco-de-Oliveira R, Flores-mendoza c, marinotti O. Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequen-ces. Med Vet Entomol. 2005; 19(2): 208-18.

Garros c, Koekemoer LL, Kamau L, awolola tS, Van bortel W, coetzee m, coosemans m, Manguin S. Restriction fragment length polymorphism method for the identification of major African and Asian malaria vectors within the Anopheles funestusand An. minimus groups. Am J Trop Med Hyg. 2004; 70(3): 260-5.

Ruiz F, Quinones ML, Erazo HF, Calle da, Alzate JF, Linton Ym. Molecular differentiation of Anopheles (Nyssorhynchus) benarrochi and An. (N.) oswaldoi from southern Colombia. Mem Inst Oswaldo Cruz. 2005; 100(2): 155-60.

Cienfuegos AV, Rosero DA, Naranjo N, Luckhart, Conn Je, Correa mm. Evaluation of a PCR-RFLP-ITS2 assay for discrimination of Anopheles species in northern and western Colombia. Acta Trop. 2011. doi10.1016/j.actatropica.2011.02.004.

Faran M, Linthicum L. A handbook of the Amazonian species of Anopheles (Nyssorhynchus) (Diptera: Culicidae). Mosq Sys. 1981; 13: 1-81.

Wilkerson R, Strickman d. Clave Ilustrada para la Identificación de las Hembras de Mosquitos de México y Centroamérica. Secretaria de Salud, Subsecretaria de Coordinación y Desarrollo, México; 1993. 46 p.

González R, carrejo n. Introducción al estudio taxonómico de Anopheles de Colombia. Claves y notas de distribución. Cali: Programa Editorial Universidad del Valle; 2007. 226 p.

Fuentelsaz c. Cálculo del tamaño de la muestra. Matronas Profesión. 2004; 5(18): 5-13.

Rosero da, Gutiérrez La, Cienfuegos AV, Jaramillo LM, correa mm. Optimización de un procedimiento de extracción de ADN a partir de Anopheles spp. Rev Colombiana de Entomol. 2010; 36(2): 260-3.

Beebe NW, Saul A. Discrimination of all members of the Anopheles punctulatus complex by polymerase chain reaction-restriction fragment length polymor-phism analysis. AM J Trop Med Hyg. 1995; 53(5): 478-81.

Pérez G, Pérez B, Mendoza DL, Blanco P. Identificación de especies de Anopheles de importancia médica en el departamento de Sucre, Colombia. Duazary 2006; 3(2): 104-9.

Gutierrez La, gonzález JJ, gómez GF, Castro MI, Rosero da, Luckhart S,Conn JE, Correa MM. Species composition and natural infectivity of anthropophilic Anopheles (Diptera: Culicidae) in the states of Córdoba and Antioquia, Northwestern Colombia. Mem Inst Oswaldo Cruz. 2009; 104(8): 1.117-24.

Fajardo M, González R, Suárez MF, López D, Wilkerson R, Mureb Ma. Morphological analysis of three populations of Anopheles (Nyssorhynchus) nuneztovari Gabaldón (Diptera: Culicidae) from Colombia. Mem Inst Oswaldo Cruz. 2008; 103(1): 85-92.

Conn J, Puertas YR, Seawright Ja. A new cytotype of Anopheles nuneztovari from western Venezuela and Colombia. J AM Mosq Control Assoc. 1993; 9(3): 294-301.

Mirabello L, Conn Je. Population analysis using the nuclear white gene detects Pliocene/Pleistocene lineage divergence within Anopheles nuneztovari in South America. Med Vet Entomol. 2008; 22(2): 109-19.

Sierra DM, Velez Id, Linton Ym. Malaria Vector Anopheles (Nyssorhynchus) nuneztovari Comprises One Genetic Species in Colombia Based on Homogenei-ty of Nuclear ITS2 rDNA. J Med Entomol. 2004; 41(3): 302-7.

Aramburú J, Ramal C, Witzig R. Malaria Reemergence in the Peruvian Amazon Region. Emerg Infect Dis. 1999; 5: 209-1.

Da Rocha Ja, de Oliveira SB, Póvoa MM, Moreira La, Krettli au. Malaria vectors in areas of Plasmodium falciparum epidemic transmission in the Amazon region, Brazil. Am. J. Trop. Med. Hyg. 2008; 78: 872-77.

Da Silva AR, Tauil PL, Bastos JL, Batista W, Pereira em, do Rosário e. Aspects of the focal transmission of malaria in the Island of São Luis, Maranhão. Rev Soc Bras Med Trop. 2006; 39(3): 250-4.

Mirabello L, Conn JE. Molecular population genetics of the malaria vector Anopheles darlingi in Central and South America. Heredity. 2006; 96: 311-21.

Mirabello L, Vineis JH, Yanoviak SP, Scarpassa VM, Póvoa mm, Padilla n, achee NL, conn Je. Microsatellite data suggest significant population structure and differentiation within the malaria vector Anopheles darlingi in Central and South America. BMC Ecol. 2008; 8: 3.

Conn JE , Mirabello L. The biogeography and population genetics of neotropical vector species. 2007. Heredity; 99: 245-6.

Moreno M, Marinotti O, Krzywinski J, Tadei WP, James aa, achee nL, conn Je. Complete mtDNA genomes of Anopheles darlingi and an approach to anopheline divergence time. Malar J 2010; 9:127.

Gutiérrez LA, Gómez GF, González JJ, Castro MI, Luckhart S, Conn JE, Correa mm. Microgeographic Genetic Variation of the Malaria Vector Anopheles darlingi Root (Diptera: Culicidae) from Córdoba and Antioquia, Colombia. Am J Trop Med Hyg. 2010; 83(1): 38-47.

Brochero H, Quinones ML. Retos de la entomología médica para la vigilancia en salud pública en Colombia: reflexión para el caso de malaria. Biomédica. 2008; 28(1): 18-24.

Beebe NW, Cooper RD, Foley DH, ellis Jt. Populations of the south-west Pacific malaria vector Anopheles farauti s.s. revealed by ribosomal DNA transcribed spacer polymorphisms. Heredity. 2000; 84 (2): 244-53.

Goswami G, Raghavendra K, nandab N, Gakhar SK, Subbarao SK. PCR-RFLP of mitochondrial cytochrome oxidase subunit II andITS2 of ribosomal DNA: Markers for the identification of members of the Anopheles culicifacies complex (Diptera: Culicidae). Acta Trop. 2005; 95: 92–9.

Van Bortel W, Sochanta t, Harbach Re, Socheat d, Roelants P, Backeljau t, coosemans m. Presence of Anopheles culicifacies B in Cambodia established by the PCR-RFLP assay developed for the identification of Anopheles minimus species A and C and four related species. Med Vet Entomol. 2002; 16(3): 329-34.