Identification of Candida species colonizing the lower respiratory tract in patients with respiratory symptoms

Authors

  • Yuliana Andrea Ochoa V. Major College of Antioquia
  • Catalina de Bedout Corporation for Biological Research
  • Karen Arango Corporation for Biological Research
  • Ángela Restrepo Corporation for Biological Research
  • Ángel González University of Antioquia

DOI:

https://doi.org/10.17533/udea.hm.15057

Keywords:

Candida spp, C. albicans, bronchoalveolar lavage, respiratory tract

Abstract

INTRODUCTION
Colonization of the lower respiratory tract by Candida spp., is considered a prerequisite for the development of Candida infection in patients with risk factors.
OBJECTIVE
To determine the different Candida species colonizing the lower respiratory tract in patients with respiratory symptoms by bronchoalveolar lavages (BAL) assessment.
MATERIALS AN DMETHODS
We conducted a descriptive study, which analyzed 61 BAL samples from patients with respiratory symptoms. BAL samples were cultured on selective media including CHROMagar Candida,  tobacco agar and hypertonic Sabouraud agar; and indentification was also performed by sugar assimilation method API 20C AUX. Additionally, we determined the minimal inhibitory concentration (MIC) for fluconazole and voriconazole. 
RESULTS
In 61 patients studied, we observed that 26 (42.6%) of them were colonized according to the following distribution: C. albicans (36.1%.), C. tropicalis (8.2%), C. krusei (3.3%), C. glabrata (3.3%), C. dubliniensis
(4.9%), C. lusitaniae (1.6%) and other Candida species (6.6%). In addition, we found that some patients were colonized by more than one Candida species. Candida krusei and C. glabrata showed a diminished susceptibility or resistance to azoles, whereas C. albicans was 100% sensitive to these antifungals.
CONCLUSIONS
These results indicate that the frequency of the Candida spp., isolates in BAL from patients with respiratory symptoms is higher than that reported in other studies.
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Author Biographies

Yuliana Andrea Ochoa V., Major College of Antioquia

Faculty of Health Sciences, Major College of Antioquia University Institution.

Catalina de Bedout, Corporation for Biological Research

Medical and Experimental Mycology Group, Corporation for Biological Research (CIB).

Karen Arango, Corporation for Biological Research

Medical and Experimental Mycology Group, Corporation for Biological Research (CIB).

Ángela Restrepo, Corporation for Biological Research

Medical and Experimental Mycology Group, Corporation for Biological Research (CIB).

Ángel González, University of Antioquia

Research Group in Basic and Applied Microbiology (MICROBA), School of Microbiology, University of Antioquia, Medellín, Colombia. 

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Published

2013-04-07

How to Cite

Ochoa V., Y. A., de Bedout, C., Arango, K., Restrepo, Ángela, & González, Ángel. (2013). Identification of Candida species colonizing the lower respiratory tract in patients with respiratory symptoms. Hechos Microbiológicos, 3(1), 21–30. https://doi.org/10.17533/udea.hm.15057

Issue

Vol. 3 No. 1 (2012)

Section

Artículos de investigación original
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