Validation of an isolation method for Legionella pneumophila by means of membrane filtration
DOI:
https://doi.org/10.17533/udea.hm.v14n1a02Keywords:
Legionella pneumophila, Celltrazone®, membrane filtration, validationAbstract
The isolation and detection of Legionella pneumophila in drinking water can be a difficult process due to the fact that some of the existing techniques do not recover the amount established by international standards and, as it is a demanding microorganism, special media are required for its isolation and growth. The objective of this study was to validate a concentration method through the implementation of the Celltrazone® equipment to achieve recovery
in BCYE agar, using drinking water samples with
different concentrations of the bacteria. To achieve this objective, three types of treatment were tested for the filter obtained after filtration by the Celltrazone® equipment, vortex, sonication and agitation; however, better results were obtained without applying any procedure to the filter and performing direct seeding. Statistical analysis showed that up to 1x102 CFU can
be concentrated with the selected method, a sensitivity of 100%, a specificity of 80% and an accuracy value of 87.5% were achieved. The membrane filtration method using the Celltrazone® equipment allowed to increase the concentration of L. pneumophila in drinking water, complying with the normalized intervals by ISO and WHO; therefore, the method is suitable for its application, ensuring reliable results.
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