Histological description of Galleria mellonella
Contribution to its implementation as an animal model
DOI:
https://doi.org/10.17533/udea.hm.v13n1a02Keywords:
Galleria mellonella , Animal Model, Histolo-gical technic, HematoxylinAbstract
Introduction:Galleria mellonella larvae are being used as an animal model to study infectious diseases. To microscopically evaluate host-pathogen interactions in vivo, an appropriate histological processing of the larvae is required to obtain good sections to assess the changes generated during infection. Researchers need to know the normal histology of the larva in order to determine if the pathogen is capable of causing disease and if new treatments are effective.Objective: This study presents the fixation procedure and histological technique of Galleria mellonella larvae that we perform routinely at the FUCS´ Histological Technique Laboratory, and the histological description of normal larvae. Methods: Galleria mellonella larvae were fixed with 100 ????L of FAATD, transverse and longitudinal sections were made, and were processed automatically, in the Technicon Doble model 2ª tissue processor. The larvae went to the embedding center to create a three-dimensional figure and finally it was cut into fine slices using the microtome. Hematoxylin Eosin (H&E) staining and photographic record were performed. Hemolymph was taken, spread on slides and colored with Wright for photographic record. Results: We show and describe the normal organs and structures of the larvae that should be analyzed in studies of microbial pathogenicity and evaluation of new molecules for the treatment of infections. Conclusion: The histological study presented is a tool that contributes to the implementation of Galleria mellonella as an animal model.
Downloads
References
[1]. Tsai CJ-Y, Loh JMS, Proft T. Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing. Virulence. 2016;7(3):214–29. DOI: 10.1080/21505594.2015.1135289
. Kavanagh K, Sheehan G. T The Use of Galleria mellonella Larvae to Identify Novel Antimicrobial Agents against Fungal Species of Medical Interest. J Fungi (Basel). 2018;4(3):113. DOI: 10.3390/jof4030113
[3]. Wojda I, Staniec B, Sułek M, Kordaczuk J. The greater wax moth Galleria mellonella: biology and use in immune studies. Pathog Dis. 2020;78(9):ftaa057. DOI: 10.1093/femspd/ftaa057
[4]. Aparecida Procópio Gomes L, Alves Figueiredo LM, Luiza do Rosário Palma A, Corrêa Geraldo BM, Isler Castro KC, Ruano de Oliveira Fugisaki L, Jorge AOC, de Oliveira LD, Junqueira JC. Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model. ScientificWorldJournal. 2016;2016:8626987. DOI: 10.1155/2016/8626987.
[5]. Cutuli MA, Petronio Petronio G, Vergalito F, Magnifico I, Pietrangelo L, Venditti N, et al. Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing. Virulence. 2019;10(1):527–41. DOI: 10.1080/21505594.2019.1621649
[6]. Villalobos-Moreno A, Agudelo M JC, Arrieta P DM. Villalobos M. A, Agudelo M. JC, Arrieta P. DM. Histología de Apis mellifera Linnaeus, 1758 (hymenoptera) como aporte entomológíco. Parte I: Regiones corporales, organización anatómica e integumento. Bol. cient. mus. hist. nat. univ. caldas. 2010;14(2):201-14. Disponible en: https://revistasojs.ucaldas.edu.co/index.php/boletincientifico/article/view/5269
[7]. Wu G, Liu Y, Ding Y, Yi Y. Ultrastructural and functional characterization of circulating hemocytes from Galleria mellonella larva: Cell types and their role in the innate immunity. Tissue Cell. 2016 Aug;48(4):297–304. DOI:10.1016/j.tice.2016.06.007.
[8]. Lu A, Zhang Q, Zhang J, Yang B, Wu K, Xie W, et al. Insect prophenoloxidase: the view beyond immunity. Front Physiol. 2014;5:252. DOI:10.3389/fphys.2014.00252
[9]. Loh JMS, Adenwalla N, Wiles S, Proft T. Galleria mellonella larvae as an infection model for group A streptococcus. Virulence. 2013;4(5):419–28. DOI: 10.4161/viru.24930
[10]. Salazar-Escobar JA, Villalobos-Moreno A. Algunas notas sobre el integumento quitinoso e iridiscente en la clase Insecta (Arthropoda: Hexapoda) . Rev. Chilena Ent. 2021;47(3). https://www.biotaxa.org/rce/article/view/70348
[11]. Barbosa P, Berry D, Kary C. Insect Histology: Practical Laboratory Techniques. John Wiley & Sons, Ltd; 2014.
[12]. Triplehorn C, Johnson N. Borror and DeLong’s Introduction to the study of insects. 7th ed. Thompson Brooks / Cole; 2005.
[13]. Chapman RF. The Insects Structure and function. Cambridge University Press; 2016.
[14]. Kludkiewicz B, Kucerova L, Konikova T, Strnad H, Hradilova M, Zaloudikova A, et al. The expansion of genes encoding soluble silk components in the greater wax moth, Galleria mellonella. Insect Biochem Mol Biol. 2018; 106:28-38. DOI:10.1016/j.ibmb.2018.11.003.
[15]. Sehnal F, Sutherland T. Silks produced by insect labial glands. Prion. 2008;2(4):145-53. DOI:10.4161/pri.2.4.7489.
[16]. Kryukova NA, Mozhaytseva KA, Rotskaya UN, Glupov V V. Galleria mellonella larvae fat body disruption (Lepidoptera: Pyralidae) caused by the venom of Habrobracon brevicornis (Hymenoptera: Braconidae). Arch Insect Biochem Physiol. 2021;106(1):e21746. DOI: 10.1002/arch.21746.
[17]. Ménard G, Rouillon A, Cattoir V, Donnio PY. Galleria mellonella as a Suitable Model of Bacterial Infection: Past, Present and Future. Front Cell Infect Microbiol. 2021;11:782733. DOI: 10.3389/fcimb.2021.782733.
[18]. Kavanagh K, Reeves EP. Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens. FEMS Microbiol Rev. 2004;28(1):101–12. DOI: 10.1016/j.femsre.2003.09.00
[19]. Vogelweith F, Moret Y, Monceau K, Thiéry D, Moreau J. The relative abundance of hemocyte types in a polyphagous moth larva depends on diet. J Insect Physiol. 2016;88:33–9. DOI: 10.1016/j.jinsphys.2016.02.010
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Hechos Microbiológicos
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
