Efectos del sistema de conservación sobre la fertilidad de oocitos de yamú (Brycon amazonicus) durante cortos períodos de almacenamiento
DOI:
https://doi.org/10.17533/udea.rccp.324048Keywords:
eggs, fertility, storage, temperature, yamúAbstract
Resumen
El objetivo fue evaluar los efectos del sistema de conservación sobre la fertilidad de oocitos de yamú (Brycon amazonicus) durante cortos períodos de almacenamiento. Se utilizaron hembras y machos adultos tratados con extracto de hipófisis de carpa para estimular la ovulación y aumentar el volumen seminal. Oocitos obtenidos de la misma hembra fueron sometidos a tres sistemas de conservación: in situ (IS, mantenidos dentro de la cavidad ovárica), ex situ a temperatura ambiente (T°A) y ex situ a temperatura de refrigeración (T°R). El diámetro ovocitario (DO) y la viabilidad fueron evaluados a los 0 (control), 15, 30, 60 y 120 min de almacenamiento. El DO fue medido con reglilla acoplada al ocular del estereomicroscopio y la viabilidad mediante prueba de fertilidad, seminando 3 g de oocitos con 250 µL de semen fresco. También fueron medidos la longitud total y el volumen del saco vitelino de las larvas. Bajo los tres sistemas de conservación, DO aumentó con el tiempo, siendo mayor (p < 0.001) que el control a partir de los 15 min de almacenamiento. Los mayores DO fueron observados en oocitos conservados a T°R (p < 0.05). Hasta 60 min de almacenamiento, en los sistemas de conservación IS y a T°A, la fertilidad no varió (p > 0.05) con respecto al control. A los 120 min de almacenamiento, todos los sistemas de conservación mostraron fertilidad menor que el control; sin embargo, en el sistema T°R disminuyó más rápidamente, siendo menor (p < 0.05) que el control a los 30 min. Aunque las larvas no presentaron evidentes alteraciones morfológicas, las del control fueron más largas (p < 0.01) que aquellas de oocitos conservados. En conclusión, oocitos ovulados de yamú pueden mantenerse viables hasta por 60 min, conservándolos en la cavidad ovárica o a temperatura ambiente. En contraste, la refrigeración deterioró rápidamente la fertilidad de oocitos ovulados.
Summary
The aim of this study was to evaluate the effects of the conservation system on eggs fertility of yamu (Brycon amazonicus) during short-term storage. Mature yamu females and males were induced to stimulated ovulation and spermiation by injection of carp pituitary extract. The eggs obtained from the same female were subjected to three different conservation systems: in situ conservation (IS, in the ovarian cavity), ex situ at room temperature (T°E) and ex situ at refrigeration temperature (T°R). The egg diameter (ED) and egg viability were evaluated at different times of storage (0 - control-, 15, 30, 60 and 120 min). The ED was measured with a rule coupled to the stereomicroscope objective, and the viability was evaluated by fertility test; for this purpose, three g of eggs were inseminated with 250 µL of fresh semen. The total larvae length and the volume of the egg yolk were measured, too. In the three conservation system, the ED increased through the time, being higher than the control (p < 0.05) from the 15 min of storage. The highest ED values were observed in eggs storage at TºR (p < 0.05). Up to 60 min of storage, in IS and T°E conservation system, the fertilization rate with respect to control did not vary (p > 0.05). In all conservation system at 120 min of storage, the fertilization rate was smaller than the control; however, in the TºR conditions the fertilization percentage decreased drastically after 30 min, being smaller (p < 0.05) than the control. Although the larvae did not show morphological alterations, the larvae control were longer than the other conservation system (p < 0.01). In conclusion, the ovulated eggs of yamu could maintain its viability up to 60 min postovulation in the ovarian cavity or at room temperature. In contrast, the refrigeration produced a drastic reduction of egg fertility in this species.
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References
Alderdice DF. Osmotic and ionic regulation in teleost eggs andlarvae. In: Hoar WS, Randall DJ, editors. Fish Physiology. ThePhysiology of developing fish, eggs and larvae, vol. 11A. London:Academic Press; 1988. p. 163-251. DOI: https://doi.org/10.1016/S1546-5098(08)60200-9
Arias JA. BiologÌa reproductiva del yam ̇ Brycon siebenthalae(PISCES: CHARACIDAE) bajo condiciones de cautiverio. Tesisde Doctorado, Facultad de Ciencias, Universidad del Valle. Cali.Colombia, 2002. 120 p.
Atencio-GarcÌa VJ. Alguns aspectos da fisiologia do yam ̇ (Bryconsiebenthalae, Eigenmann 1912). RevisiÛn teÛrica acerca de laespecie yam ̇, Universidade Federal de Santa Catarina,FlorianÛpolis. 1999. 20 p.
Chao Nai-Hsien, Chiu Liao I. Cryopreservation of finfish andshellfish gametes and embryos. Aquaculture 2001; 197:161-89. DOI: https://doi.org/10.1016/B978-0-444-50913-0.50011-4
Coser AML, Godinho HP. Capacidade de fertilizaÁ„o de ovÛcitose sÍmen de curimat„-pac ̇ (Prochilodus marggravii) em condiÁıesexperimentais. In: AssociaÁ„o Mineira de Aquaicultura (AMA),1987. Resumos dos encontros 1982-87. BrasÌlia: CODEVASF,1988. p. 112-13.
Craik JCA, Harvey SM. Biochemical changes associated withoverripening of the eggs of rainbow trout Salmo gairdneriRichardson. Aquaculture 1984; 37:347-57. DOI: https://doi.org/10.1016/0044-8486(84)90299-0
Cruz-Casallas PE, Pardo-Carrasco SC, Arias-Castellanos JA,Lombo-Castellanos PE, Lombo-RodrÌguez DA, et al.Cryopreservation of Yam ̇ Brycon siebenthalae Milt. J. WorldAquacult Soc 2004; 35:529-35. DOI: https://doi.org/10.1111/j.1749-7345.2004.tb00120.x
Davenport J, L ̄nning S, Kj ̄rsvik E. Osmotic and structuralchanges during early development of eggs and larvae of the cod,Gadus morhua L. J Fish Biol 1981; 19:317-31. DOI: https://doi.org/10.1111/j.1095-8649.1981.tb05835.x
Dushkina LA. Viability of herring (Clupea) eggs and fertilizingcapacity of herring sperm stored under various conditions. JIchthyol 1975; 15:423-29.
Espinach-Ros A, Amutio VG, Mestre Arceredillo JP, Orti G, NaniA. Induced breeding of the South American catfish, Rhamdiasapo (C.& V.). Aquaculture 1984; 37:141-46. DOI: https://doi.org/10.1016/0044-8486(84)90071-1
Formacion MJ, Hori R, Lam TJ. Overripening of ovulated eggs ingoldfish: I. Morphological changes. Aquaculture 1993; 114:155-68. DOI: https://doi.org/10.1016/0044-8486(93)90258-Z
Fortuny A, Espinach Ros A, Amutio VG. Hormonal induction offinal maturation and ovulation in the s·balo, Prochilodus platensisHolmberg: Treatments, latency and incubation times and viabilityof ovules retained in the ovary after ovulation. Aquaculture 1988;73:373-81. DOI: https://doi.org/10.1016/0044-8486(88)90070-1
Ginsburg AS. Fertilization in fishes and the problem of polyspermy.Keter Press, Jerusalem (Translated from Russian, available fromU.S. Dept. of Commerce, NTIS, Springfield, Virginia). 1972. 253 p.
Harvey B, Kelley N. Short-term storage of Sarotherodonmossambicus ova. Aquaculture 1984; 37:391-95. DOI: https://doi.org/10.1016/0044-8486(84)90304-1
Isayeva A, Zhang T, Rawson DM. Studies on chilling sensitivityof zebrafish (Danio rerio) oocytes. Cryobiology 2004; 49:114-22. DOI: https://doi.org/10.1016/j.cryobiol.2004.05.005
Junqueira CAP. Efeito da temperatura no desenvolvimento inicialde larvas de ìCurimbat·î Prochilodus scrofa STEINDACHNER,1882 (CHARACIFORMES, PROCHILODONTIDAE).DissertaÁ„o de Mestrado em Acuicultura. Universidade Federal deSanta Catarina. Brasil. 1999. 83 p.
Kj ̄rsvik E, L ̄nning S. Effects of egg quality on normal fertilizationand early development of the cod, Gadus morhua L. J Fish Biol1983; 23:1-12. DOI: https://doi.org/10.1111/j.1095-8649.1983.tb02877.x
Lahnsteiner F, Weismann T. Changes in eggs of brown trout,rainbow trout, and grayling during short-term storage. North AmJ Aquacult 1999; 61:213-19. DOI: https://doi.org/10.1577/1548-8454(1999)061<0213:CIEOBT>2.0.CO;2
Leung LKP, Jamieson BGM. Live preservation of fish gametes.In: Jamieson BGM, editors. Fish evolution and systematics:evidence from spermatozoa. Cambridge: Cambridge UniversityPress, 1991. p. 245-69.
Linhart O, Billard R. Survival of ovulated oocytes of the Europeancatfish (Silurus glanis) after in vivo and in vitro storage orexposure to saline solutions and urine. Aquat Living Resour 1995;8:317-22. DOI: https://doi.org/10.1051/alr:1995033
Linhart O, Kudo S, Billard R, Slechta V, Mikodina EV. Morphology,composition and fertilization of carp eggs: a review. Aquaculture1995; 129:75-93. DOI: https://doi.org/10.1016/0044-8486(94)00230-L
Liu XH, Zhang T, Rawson DM. Effect of cooling rate and partialremoval of yolk on the chilling injury in zebrafish (Danio rerio)embryos. Theriogenology 2001; 55:1719-31. DOI: https://doi.org/10.1016/S0093-691X(01)00515-5
Mommsen TP, Walsh PJ. Vitellogenesis and oocyte assembly. In:Hoar WS, Randall DJ, editors. Fish Physiology. The Physiologyof developing fish, eggs and larvae, vol. 11A. London: AcademicPress; 1988. p. 347-406. DOI: https://doi.org/10.1016/S1546-5098(08)60202-2
Rizzo E, Godinho HG, Sato Y. Short-term storage of oocytesfrom the neotropical teleost fish Prochilodus marggravii.Theriogenology 2003; 60:1059-70. DOI: https://doi.org/10.1016/S0093-691X(03)00108-0
Rizzo E. Biologia de ovos de peixes neotropicais de ·gua doce:Estudos comparativo e experimental, com Ínfase na an·lise dasuperfÌcie e do aparelho micropilar. Tese de Doutorado em CiÍncias.Universidade Federal de Minas Gerais. Belo Horizonte. Brasil.2001. 103 p.
Stoss J. Fish gamete preservation and spermatozoan physiology.In: Hoar WS, Randall DJ, Donaldson EM, editors. Fish Physiology.Vol. 9B. New York: Academic Press; 1983. p. 305-51. DOI: https://doi.org/10.1016/S1546-5098(08)60306-4
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