Inducción a la maduración final y ovulación del yamú Brycon amazonicus con EPC y mGnRH-a

Authors

  • Sandra C. Pardo Carrasco
  • José A. Arias
  • Héctor Suarez-Mahecha
  • Pablo Cruz Casallas
  • Wálter Vásquez Torres
  • Víctor J. Atencio García
  • Evoy Zaniboni Filho

DOI:

https://doi.org/10.17533/udea.rccp.324040

Keywords:

Bryconinae, culture, reproduction

Abstract

Resumen

En hembras de yamú Brycon amazonicus de primera maduración sexual (2 años de edad), con un peso corporal (PC) de 1.500 ± 1 g y longitud total (LT) de 42.6 ± 0.1 cm, fueron probados dos inductores hormonales, extracto de pituitaria de carpa (EPC) y análogo de mamífero de la hormona liberadora de gonadotropinas (mGnRH-a). Ocho tratamientos fueron realizados: seis con mGnRH-a (10, 15 y 20 µg/kg en una sola aplicación y 10, 15 y 20 µg/kg en dos aplicaciones del 10 y 90%, respectivamente), una con EPC (5.5 mg/kg en dos aplicaciones, 10% y 90%, con un intervalo de 12 horas) y un control (solución salina 0.9%). Las hembras inducidas con mGnRH-a y las hembras control no desovaron. El 83.3% de las hembras inducidas con EPC desovaron a las 6.7 ± 0.14 h a 26.9 ± 0.18 ºC (temperatura del agua), con un promedio de 117.6 ± 6.8 g de huevos por hembra y 1469.6 ± 34.1 huevos por gramo. La tasa de fertilidad presentó un promedio de 55.3 ± 6.1% seis horas después de la fertilización (HDF) y 51 ± 5.8% de sobrevivencia embrionaria a 10 HDF. La eclosión sucedió a las 12 HDF. Solamente las hembras inducidas con EPC presentaron un crecimiento significativo del diámetro ovocitario (p < 0.05) de 1086 a 1178 µm.

Summary

In females of yamu Brycon amazonicus of first sexual maturation (two years old, body weight (BW) 1.500 ± 1 g and total length (TL) 42.6 ± 0.1 cm), two hormonal inductors were evaluated, carp pituitary extract (CPE) and mammalian gonadotropin-releasing hormone analogue (mGnRH-a). Eight treatments were conducted: six with mGnRH-a (10, 15 and 20 µg/kg BW in single injection and 10, 15 and 20 µg/kg BW in two injections of 10 and 90%, respectively), one with CPE (5.5 mg/kg BW in two applications, 10% and 90%, with an interval of 12 h), and one control (saline solution 0.9%). Females induced with mGnRH-a and females controls did not spawn. The 83.3% of the females induced with CPE spawned at the 6.7 ± 0.14 h at 26.9 ± 0.18 ºC (water temperature), with a mean of 117.6 ± 6.8 g per egg volume per female, and 1469.6 ± 34.1 eggs per gram. The fertility rate showed a mean of 55.3 ± 6.1% 6 hours after fertilization (HAF), and 51 ± 5.8% of embryonic survival to 10 HAF. The hatching happened at 12 HAF. Only females induced with CPE presented a significant growth of the diameter (p < 0.05) of 1086 to 1178 µm.

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References

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Published

2016-07-21

How to Cite

Pardo Carrasco, S. C., Arias, J. A., Suarez-Mahecha, H., Cruz Casallas, P., Vásquez Torres, W., Atencio García, V. J., & Zaniboni Filho, E. (2016). Inducción a la maduración final y ovulación del yamú Brycon amazonicus con EPC y mGnRH-a. Revista Colombiana De Ciencias Pecuarias, 19(2), 7. https://doi.org/10.17533/udea.rccp.324040

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