Valoración de la citología para el diagnóstico de tumores en caninos
DOI:
https://doi.org/10.17533/udea.rccp.324349Keywords:
cytodiagnosis, fine needle aspiration biopsy (FNAB), histopathology, neoplasiaAbstract
Summary
Cytology is a controversial diagnostic tool in veterinary medicine. In Colombia there are no studies about its reliability as a diagnostic tool. The porpoise of this study was to compare accuracy of the cytology with respect to histopathology for the diagnosis of mammary, cutaneous and subcutaneous tumors in dogs. We performed an observational analytic study from July to October 2006. In this study 42 biopsies and 35 fine needle aspiration biopsies (FNAB) of canine mammary, cutaneous and subcutaneous tumors were observed. Samples were obtained from four veterinary clinics in Medellin, Colombia. Samples were processed by rutine methods and they were stained with hematoxilin-eosin and Wright- Giemsa stains. Cytology evaluation was performed by a pathologist and the FNAB by cytology students. The FNAB has a high sensibility to determine malignancy (76.47%), a specificity of 93.33%, positive and negative predictive values of 84.38 and 92.86%, respectively. The concordance between the final diagnosis obtained from FNAB and histopathology was 79%. Cytodiagnosis is a reliable tool to determine the malignancy of mammary cutaneous and subcutaneous tumors in dogs in the pathology laboratory of "Universidad de Antioquia".
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Animales de compañía: cómo nos comportamos con ellos: encuesta [editorial]. Rev Consumer Eroski [Octubre 19 de 2006] URL: http://revista.consumer.es/web/es/20000901/actualidad/tema_de_portada/.2.Barr R. Cutaneous Cytology. J Am Acad Dermatol 1998; 10:163-180.
Barton CL. Cytologyc diagnosis of cutaneous neoplasia: an algorithimic approach. Comp Cont Ed Pract Vet 1987; 9:20-33.
Cabrera A, G Susana, Medina J, Ortega V, Pérez G,et al. Diagnóstico de la linfadenitis tuberculosa en pacientes VIH: validación de la punción-aspiración con aguja fina. Resultados preliminares. VITAE Acad Biom Dig 2005; [Mayo 28 de 2006] URL: http://caibco.ucv.ve/caibco/caibco/vitae/VitaeVeintidos/Congreso/ArchivosPDF/Codigo08.pdf
Chalita MC, Matera JM, Alves MT, Longatto Filho A. Non-aspiration fine needle cytology and its histologic correlation in canine skin and soft tissue tumors. Anal Quant Cytol Histol 2001; 23:395-399.
Ciappesoni JL, Duchene A., Zaccagnini A., Ontiveros L. Presentación ósea de un mastocitoma grado 3 en un Canino. Terceras jornadas de la Facultad de Ciencias Veterinarias, Universidad de Buenos Aires 2005. [Noviembre 12 de 2006] URL: http://www.fvet.uba.ar/invet/suplemento3j.pdf
Clinkenbeard KD, Cowell RL. Características citológicas de las neoplasias malignas. Waltham Focus 1994; 4:2-8.
Cruz NA, Martinez de Larios NH. La biopsia por aspiración con aguja fina en glándula mamaria: diagnostico citológico y concordancia histológica y clínica. Rev Hospital General M Gea Gonzáles 2002; 5:79-84
Devet H. Observer reliability and agreement. In: Armitage P, Colton T, editores. Encyclopedia of Biostatistics. Vol 4. Chichester: John Wiley & Sons; 1998. p.3123-7.
Fabre W. Needle aspiration biopsy: past, present and future. Human Pathol 1989; 20:504-517. DOI: https://doi.org/10.1016/0046-8177(89)90242-6
Fan TM, Daliwahl RS. Neoplasia in dogs and cats: Part 1, Vet Medic 2001; 96:918-949
García del Moral R. Laboratorio de Anatomía Patológica. Barcelona: Interamericana McGraw – Hill; 1993.
Garzón Alzate A., Aranzazu Taborda DA., Rodríguez B. de J., Álvarez Yépez G. Caracterización de los tumores cutáneos caninos diagnosticados en el laboratorio de patología animal de la Universidad de Antioquia entre 1994-2003. Rev Colomb Cienc Pec 2005; 18:386.
Ghisleni G, Roccabianca P, Ceruti R, Stefanello D, Bertazzolo W, et al. Correlation between fine-needle aspiration cytology and histopathology in the evaluation of cutaneous and subcutaneous masses from dogs and cats. Vet Clin Pathol 2006; 35:24-30. DOI: https://doi.org/10.1111/j.1939-165X.2006.tb00084.x
Giraldo GE, Aranzazu DA, Rodríguez B de J, Pérez MM, Ramírez MC. Caracterización de las Regiones Organizadoras Nucleolares coloreadas con Plata (AgNORs) en tumores cutáneos caninos. Rev Colomb Cienc Pec 2003; 16:132-138
Goldschmidt MH, Dunstan RW, Stannard AA, Tscharner C, Walter EJ, et. al., editors. Histological classification of epithelial and melanocytic tumours of the skin of domestic animals. 2nd ed. Vol III, Washington: Armed Forces Institute of Pathology; 1998.
Griner PF, Mayewski RJ, Mushlin AI, Greenland P. Selection and interpretation of diagnostic tests and procedures. Ann Intern Med 1981; 94:557-563.
Gutiérrez Vidrio RM. Cáncer de piel. Rev Fac Med UNAM 2003; 46:4
Jack EM, editor. Tumors in domestic animals. Los Angeles: California: Berkeley, 1990.
Kristensen AB, Feldman BF. Cytology in veterinary practice: a review of clinical cytology - body fluids, lymph nodes and skin neoplasms. Nord Vet Med 1986; 38:321-332.
Larkin, H.A. Veterinary cytology. Differentiation of malignant lym-phoma and other tumours of animals. Irish Vet Jou 1994; 47:161-167.
Larkin, HA. Veterinary cytology. Fine needle aspiration masses of swellings on animals. Iris Vet Jou 1994; 47:65-73.
Maidana HR, Macció OA, Merlo WA, Pérez Valega E. Neoplasias cutáneas en caninos. Aspectos clínicos y anatomopatológicos. Informe de avance. XXIII Sesión de Comunicaciones Científicas. Universidad Nacional del Nordeste 2002; [Noviembre 20 de 2006] URL: http://www.vet.unne.edu.ar/ComCientificas/sesion-03/posters/SesionCom36.doc
Meinkoth JH, Allison R. Sample collection and handling: getting accurate results. Vet Clin North Am Small Anim Pract 2007; 37:203-219. DOI: https://doi.org/10.1016/j.cvsm.2006.11.008
Misdorp W, Else RW, Hellmen E, Lipscomb TP, editors. Histological classification of mammary tumours of the dog and the cat. 2nd Series Vol VII. Washington: Armed Forces Institute of Pathology, 1999.
Pardanani A, Akin C, Valent P. Pathogenesis, clinical features, and treatment advances in mastocitosis. Best Pract Res Clin Haemat 2006; 19:595-615. DOI: https://doi.org/10.1016/j.beha.2005.07.010
Piñol A., editor. Citodiagnóstico de los tumores y reticulosis de la piel. Barcelona: Syntex Iberica; 1973.
Prophet EB, Mills B, Arrington JB, Sobin LH, editors. Armed Forces Institute of Pathology. Laboratory Methods in Histotechnology. American Registry of Pathology; 1992.
Raskin RE, Meyer DJ. Atlas of canine and feline cytology. Philadelphia: Saunders Company; 2001. p.35-92
Rebar, AH. Diagnostic cytology in veterinary practice: current status and interpretative principles. In: Kirk R, editor. Current veterinary therapy. Small animal practice; 1980. p.16-27.
Rosciani AS, Merlo WA, Moya G, Báez LE, Lockett AD, et. al. Consideración de variables clínicas en hembras caninas con tumores mamarios. Universidad Nacional del Nordeste. Comun Cient y Tecno, Tecnológicas UNNE; 2004.
Scout DW, Millar JR, Griffin C, editors. Muller and Kirk ́s Small animal dermatology. 6th ed. Oxford, Elsevier Saunders; 2000.
Taube A. Sensitivity, Specificity and Predictive Valves, graphical approach, Stat Med 1986; 5:585-591 DOI: https://doi.org/10.1002/sim.4780050606
Thompson WD, Walters SD. A reapraisal of the Kappa coefficient. J Clin Epidemiol 1998; 41:949-958. DOI: https://doi.org/10.1016/0895-4356(88)90031-5
Tobón Acosta JL, comunicación personal. Médico Patólogo Veterinario de la Universidad de Antioquia, 2007.
Tvedten H, Cowell RL. Citologia de masas neoplásicas e inflamatórias. En: Willard MD, Tvedten H, Turnwald G, editores. Diagnostico Clínico patológico práctico en los pequeños animales. 4ta ed. Buenos Aires: Inter-Médica; 2004. p. 35
Tyler RD, Cowell RL, Baldwin CJ. Introduction. In: Cowell RL, Tyler RD, editors: Diagnostic cytology and hematology of the dog and cat. 2nd ed. St. Louis: Mosby, Inc. 1999; 1-19.
Vasallo Pastor N, Peña Cedeño AL, Rodríguez Pérez A. Tumores malignos en nuestro medio. estudio de diez años. Rev Cubana Obstet Ginecol 1999; 25:190-193
Vergara TF, Flores RP. Tumor mamario canino y felino. Estudio recapitulativo. Rev AMMVEPE 2000; 11:85-93.
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