Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen

  • Elizabeth Varela-Giraldo Jaime Isaza Cadavid Colombian Polytechnic
  • Alexandra Úsuga-Suárez CES University
  • Juan E. Duque-Cortés Jaime Isaza Cadavid Colombian Polytechnic
  • Jorge Gómez-Oquendo Jaime Isaza Cadavid Colombian Polytechnic
  • Giovanni Restrepo-Betancur National University of Colombia
Keywords: bull, cryopreservation, cryoprotectant, egg yolk, extender, low-density lipoproteins, semen, trehalose


Background: In artificial insemination, chicken egg yolk is added to bovine semen to protect it during the cryopreservation process, although it contains substances that can affect the microbiological quality and metabolism of sperm. Objective: To evaluate post-thaw quality of bovine cryopreserved semen added with centrifuged and non-centrifuged egg yolk, low-density lipoproteins (LDL), and trehalose (T). Methods: Ten ejaculates from five bulls were cryopreserved under the treatments T1: pure egg yolk (PEY) at 20% v/v, T2: centrifuged egg yolk (CEY) at 20% v/v, T3: LDL at 8% v/v, T4: T at 100 mM, and T5: T at 100 mM plus LDL at 8% v/v (TLDL). Spermatic motility and kinetics, functional membrane integrity (FMI), structural membrane integrity (SMI), sperm vitality (SV) and abnormal morphology (AM) were assessed using the Sperm Class Analyzer (SCA®) system, hypoosmotic test (HOST), SYBR/PI probes, and eosin–nigrosin staining, respectively. A completely randomized design was used. Normal distribution of the variables was validated through the Kolmogórov– Smirnov test. A generalized linear model was used to determine sources of variation. Means were compared using the Tukey test. Results: Inclusion of CEY or LDL had a similar effect on sperm protection, and were superior for motility, kinetics and membrane integrity compared to the other treatments (p<0.05). CEY was superior for progressive motility (p<0.05). The cryoprotective action of LDL was similar to TLDL for motility and kinetics, SMI, SV, and AM (p<0.05). Inclusion of PEY and T resulted in the lowest semen quality (p<0.05). The use of T resulted in a reduction in FMI and SMI (p<0.05). No differences in AM between treatments were found (p>0.05). Conclusions: Egg yolk can be replaced by centrifuged egg yolk or low-density lipoproteins in the freezing extender used for bovine semen used in artificial insemination.

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Author Biographies

Elizabeth Varela-Giraldo, Jaime Isaza Cadavid Colombian Polytechnic

Jaime Isaza Cadavid Colombian Polytechnic, Faculty of Agrarian Sciences, Medellín, Colombia.

Alexandra Úsuga-Suárez, CES University

CES University, Faculty of Veterinary Medicine and Zootechnics, Medellín, Colombia.

Juan E. Duque-Cortés, Jaime Isaza Cadavid Colombian Polytechnic

Jaime Isaza Cadavid Colombian Polytechnic, Faculty of Agrarian Sciences, Medellín, Colombia.

Jorge Gómez-Oquendo, Jaime Isaza Cadavid Colombian Polytechnic

Jaime Isaza Cadavid Colombian Polytechnic, Faculty of Agrarian Sciences, Medellín, Colombia.

Giovanni Restrepo-Betancur, National University of Colombia

National University of Colombia, Faculty of Agrarian Sciences, Medellín, Colombia.


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How to Cite
Varela-Giraldo E., Úsuga-Suárez A., Duque-Cortés J. E., Gómez-Oquendo J., & Restrepo-Betancur G. (2020). Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen. Revista Colombiana De Ciencias Pecuarias, 34(3), 200-211. https://doi.org/10.17533/udea.rccp.v34n3a03
Original research articles