Asynchrony between in vivo and in vitro rabbit embryos
Keywords:assisted reproduction, embryo culture, embryonic asynchrony, embryonic development, embryo synchronization, embryo transfer, in vitro embryo production, in vivo embryo production, morulae, rabbits
Background: Comparative features of embryos developed under in vitro and in vivo conditions are particularly important in designing embryo transfer procedures that fulfil embryo-recipient synchronization requirements. Objective: To determine the degree of asynchrony in rabbit embryo development between cultured and in vivo embryos. Methods: A total of 55 non-lactating multiparous female rabbits were used. Embryos were classified as 16-cells or early morulae at 48 hours post-coitum (hpc). Embryos were cultured during 30 or 32 h and embryo development was compared with in vivo embryos of 72 hpc. In vitro and in vivo embryos at 72 hpc were classified as early or compacted morulae. Bayesian statistics was used. Difference between in vivo and in vitro embryos and the actual probability of the difference between the in vivo and in vitro embryo higher than zero (P) was estimated. Results: The percentage of compacted morulae was higher in in vivo embryos than in in vitro embryos with +6 h of asynchrony (73.5 and 32.8%, P=1.00). But the percentage of compacted morulae was similar with +8 h asynchrony. Conclusions: In vitro embryos delay their development by + 8 hours compared to in vivo embryos.
Adams CE. The development of rabbit eggs after culture in vitro for 1-4 days. J Embryol Exp Morphol 1970; 23: 21-34.
Argente MJ, García ML, Zbyňovská K, Petruška P, Capcarová M, Blasco A. Correlated response to selection for litter size environmental variability in rabbits’ resilience. Animal 2019; 13: 2348-2355. DOI: https://doi.org/10.1017/S1751731119000302
Carney EW, Foote RH. Effects of superovulation, embryo recovery, culture system and embryo transfer on development of rabbit embryos in vivo and in vitro. J Reprod Fertil 1990; 89: 543-551. DOI: https://doi.org/10.1530/jrf.0.0890543
Fowler KE, Mandawala AA, Griffin DK, Walling GA, Harvey SC. The production of pig preimplantation embryos in vitro: Current progress and future prospects. Reprod Biol 2018; 18: 203-211. DOI: https://doi.org/10.1016/j.repbio.2018.07.001
Gardner DK. Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture. Theriogenology 1998; 49: 83-102. DOI: https://doi.org/10.1016/s0093-691x(97)00404-4
García ML. Embryo manipulation techniques in the rabbit. New Insights into Theriogenology. Edited by Rita Payan-Carreira; 2018. DOI: https://doi.org/10.5772/intechopen.81089
Hegele‐Hartung C, Fischer B, Beier HM. Development of preimplantation rabbit embryos after in vitro culture and embryo transfer: An electron microscopic study. Anat Rec 1988; 220: 31-42. DOI: https://doi.org/10.1002/ar.1092200105
Lonergan P, Fair T, Forde N, Rizos D. Embryo development in dairy cattle. Theriogenology 2016; 86: 270-277. DOI: https://doi.org/10.1016/j.theriogenology.2016.04.040
Saenz-de-Juano MD, Naturil-Alfonso C, Vicente JS, Marco-Jiménez F. Effect of different culture systems on mRNA expression in developing rabbit embryos. Zygote 2011; 21: 103-109. DOI: https://doi.org/10.1017/S0967199411000414
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