Tasas de preñez y nacimientos después de la vitrificación de embriones Nelore (Bos indicus) producidos in vitro bajo condiciones de campo
DOI:
https://doi.org/10.17533/udea.rccp.v32n1a05Palabras clave:
criobiología, crioinjuria, criotolerancia, embriones sobrantes, reproducción de ganado, transferencia de embrionesResumen
Antecedentes: La criopreservación se caracteriza por el mantenimiento de la viabilidad celular a bajas temperaturas, resultando en reducido metabolismo y actividad enzimática intracelular, lo que permite la preservación del material genético por períodos de tiempo indefinidos. Los embriones sometidos a ésta técnica sufren daños morfológicos y funcionales considerables, dando como resultado una sobrevivencia y tasas de desarrollo reducidas. Objetivo: Evaluar la tasa de preñez a partir de embriones Nelore (Bos indicus) producidos in vitro (IVP) después de la vitrificación bajo condiciones de campo. Métodos: Embriones IVP en los estadios de blastocisto (Bl) y blastocisto expandido (Bx) se transfirieron en fresco (n= 137) o después de la vitrificación (n= 127). Resultados: La tasa de preñez a los 35 d fue menor para los embriones transferidos en fresco en fase Bl (41,6) en relación con los Bx (60,9) (p0,05). Las pérdidas de preñez a los 60 d fueron similares (p>0,05) tanto para embriones en fresco en Bl (3,1) y Bx (4,8) como para los vitrificados (Bl: 1,9 y Bx: 4,7). Las tasas de nacimiento fueron similares entre los grupos (p>0,05). Conclusión: Las tasas de preñez y nacimiento de embriones IVP vitrificados de Nelore (Bos indicus) bajo condiciones de campo son semejantes a las de embriones en fresco.
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Andreoti M, Spegiorin MR, Cunha CEA. Taxa de prenhez de embriões bovinos tardios transferidos no dia 8 de cultivo. Congresso brasileiro de reprodução animal, Colegio Brasileiro de Reprodução Animal CBRA (Belo Horizonte): Colégio Brasileiro de Reprodução Animal CBRA 2009; 18:352-352.
Arav A, Yavin S, Zeron Y, Natan D, Dekel I, Gacitua H. New trends in gamete’s cryopreservation. Mol Cell Endocrinol 2002; 187:77-81. http://www.sciencedirect.com/science/article/pii/S0303720701007006.
Chian RC, Kuwayama M, Tan L, Tan J, Kato O, Nagai T. High survival rate of bovine oocytes matured in vitro following vitrification. J Reprod Dev 2004; 50:685-696. https://www.jstage.jst.go.jp/article/jrd/50/6/50_6_685/_pdf.
Dattena M, Accardo C, Pilichi S, Isachenko V, Mara L, Chessa B, Cappai P. Comparison of different vitrification protocols on viability after transfer of ovine blastocysts in vitro produced and in vivo derived. Theriogenology 2004; 62:481-493. http://www.sciencedirect.com/science/article/pii/S0093691X03004059.
Kocyigit A, Cevik M. Effects of leukemia inhibitory factor and insulin-like growth factor-I on the cell allocation and cryotolerance of bovine blastocysts. Cryobiology 2015; 71:64-69. http://www.sciencedirect.com/science/article/pii/S0011224015001741.
Kuwayama M, Vajta G, Ied AS, Kato O. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online 2005; 11:608-614. http://www.sciencedirect.com/science/article/pii/S1472648310611698.
Leibo SP, Loskutoff M. Cryobiology of in vitro-derived bovine embryos. Theriogenology 1993; 39:81-94. http://www.sciencedirect.com/science/article/pii/0093691X9390025Z.
Leibo SP, Martino A, Kobayashi S, Pollard JW. Stage dependent sensitivity of oocytes and embryos to low temperatures. Anim Reprod Sci 1996; 42:43-53. http://www.sciencedirect.com/science/article/pii/0378432096015436.
Mara L, S Casu, A Carta, M Dattena. Cryobanking of farm animal gametes and embryos as a means of conserving livestock genetics. Anim Reprod Sci 2013; 138:25-38. http://www.sciencedirect.com/science/article/pii/S037843201300047X.
Massip A. Cryopreservation of embryos of farm animals. Reprod Domest Anim 2001; 36:49-55. http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0531.2001.00248.x/full.
Morató R, Izquierdo D, Paramio MT, Mogas T. Embryo development and structural analysis of in vitro matured bovine oocytes vitrified in flexipet denuding pipettes. Theriogenology 2008; 70:1536-1543. https://www.ncbi.nlm.nih.gov/pubmed/18755504.
Mucci N, Aller J, Kaiser GG, Hozbor F, Cabodevila J, Alberio RH. Effect of estrous cow serum during bovine embryo culture on blastocyst development and cryotolerance after slow freezing or vitrification. Theriogenology 2006; 65:1551-1562. http://www.sciencedirect.com/science/article/pii/S0093691X05003845.
Pereira LC, Ferreira-Silva, JC, Cantanhede LF, Veloso Neto HF, Andrade JC, Moura MT, & Oliveira MAL (2016). Viability of in vitro produced embryos of gyr cattle (bos indicus) after cryopreservation by vitrification under field conditions. Bol Ind Anim 73(2), 159-164. http://www.iz.sp.gov.br/pdfsbia/1466788221.pdf.
Prentice JR, Anzar M. Cryopreservation of mammalian oocyte for conservation of animal genetics. Vet Med Int 2010, Article ID 146405. https://www.hindawi.com/journals/vmi/2011/146405/.
Randi F, Fernandez-Fuertes B, McDonald M, Forde N, Kelly AK, Bastos Amorin H, Muniz de Lima E, Morotti F, Marcondes Seneda M, Lonergan P. Asynchronous embryo transfer as a tool to understand embryo-uterine interaction in cattle: is a large conceptus a good thing? Reprod Fert Dev 2015; 28:1999-2006. http://www.publish.csiro.au/rd/RD15195.
Sanches BV, Marinho LS, Filho BD, Pontes JH, Basso AC, Meirinhos ML, Silva-Santos KC, Ferreira CR, Seneda MM. Cryosurvival and pregnancy rates after exposure of IVF-derived Bos indicus embryos to forskolin before vitrification. Theriogenology 2013; 80:372-737. http://www.sciencedirect.com/science/article/pii/S0093691X13001817.
Scanavez AL, Campos CC, Santos RM. Taxa de prenhez e de perda de gestação em receptoras de embriões bovinos produzidos in vitro. Arq Bras Med Vet Zootec 2013; 65:722-728. http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000300017.
Sudano MJ, Paschoal DM, Rascado TS, Magalhães LCO, Crocomo LF, Lima-Neto JF, Landim-Alvarenga FC. Lipid content and apoptosis of in vitro-produced bovine embryos as determinants of susceptibility to vitrification. Theriogenology 2011; 75:1211-1220. http://www.sciencedirect.com/science/article/pii/S0093691X10006266.
Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H. Open pulled straw (ops) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 2010; 51:53-58. http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2795(199809)51:1%3C53::AID-MRD6%3E3.0.CO;2-V/epdf.
Vajta G, Kuwayama M. Improving cryopreservation systems. Theriogenology 2006; 65:236-244. http://www.sciencedirect.com/science/article/pii/S0093691X05004164.
Vajta G, Holm P, Greve T, Callesen H. Factors affecting survival rates of in vitro produced bovine embryos after vitrification and direct in-straw rehydration. Anim Reprod Sci 1996; 45(3):191-200.http://www.sciencedirect.com/science/article/pii/S0378432096015837.
Viana JHM, Siqueira LGB, Palhão MP, Camargo LSA. Use of in vitro fertilization technique in the last decade and its effect on Brazilian embryo industry and animal production. Acta Sci Vet 2010; 38:661-674. http://www.ufrgs.br/actavet/38-suple-2/23_SBTE_JHENRIQUE.pdf.
Visintin JA, Martins JFP, Bevilacqua EM, Mello MRB, Nicácio AC, Assumpção MEOA. Cryopreservation of Bos taurus vs Bos indicus embryos: are they really different? Theriogenology 2002; 57:345-359. http://www.sciencedirect.com/science/article/pii/S0093691X01006756.
Wohlres-Viana AS, Pereira MM, Viana JHM, Machado MA, Camargo LSA. Comparison of gene expression in Bos indicus and Bos taurus embryos produced in vivo or in vitro. Livest Sci 2011; 140:62-67. http://www.sciencedirect.com/science/article/pii/S1871141311000679.
Yavin S, Arav A. Measurement of essential physical properties of vitrification solutions. Theriogenology 2007; 67:81-89. http://www.sciencedirect.com/science/article/pii/S0093691X06005036.
Yavin S, Aroyo A, Roth Z, Arav A. Embryo cryopreservation in the presence of low concentration of vitrification solution with sealed pulled straws in liquid nitrogen slush. Hum Reprod 2009; 24:797-804. https://academic.oup.com/humrep/article/24/4/797/630673/Embryo-cryopreservation-in-the-presence-of-low.
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