Variabilidade genotípica e alélica do gene calpaína CAPN1-316 no gado Crioulo Leiteiro Tropical
DOI:
https://doi.org/10.17533/udea.rccp.v38n1a1Palavras-chave:
calpaína, Crioulo Leiteiro Tropical, gado, laticínios tropicais, maciez da carne, marcadores moleculares, microssatélites, raças adaptadas, recursos genéticos, variabilidade genéticaResumo
Antecedentes: Por meio de marcadores moleculares, genes e suas variantes relacionados à produção de leite e carne em bovinos podem ser identificados. O gene calpaína CAPN1-316 tem sido particularmente relacionado a maciez da carne. Machos da raça crioulo Leiteiro Tropical não utilizados como reprodutores são destinados à produção comercial de carne. Objetivo: Estimar as frequências genotípicas e alélicas do gene calpaína CAPN1-316, relacionado com a maciez da carne, na raza creole Leiteiro Tropical. Métodos: Microssatélites de marcadores moleculares de repetições únicas (SSR) de DNA foram obtidos de amostras de sangue de 423 animais de raça pura em três fazendas, para identificar variantes do gene CAPN1-316 por reação em cadeia da polimerase (PCR). As frequências genotipicas e alélicas dentro das populações, distância genética, heterozigosidade e teste de Xi-quadrado (X2) foram realizados usando o POPGENE. Resultados: As frequências genotípicas de toda a população foram CC 0,31, GG 0,18 y CG 0,51. As frequências alélicas da variante C, favorável a maciez da carne, foram 0,62, 0,60 e 0,52 para cada fazenda. As frequências genotípicas e alélicas do polimorfismo do gene CAPN1-316 ficaram em equilíbrio Hardy-Weinberg (X2, p>0,05). O genótipo CC apresentou indicação de melhor maciez. Conclusões: No gado crioulo Leiteiro Tropical, a variante C do CAPN1-316, favorável à maciez da carne, teve maior frequência que a variante G.
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