Clonal mucropropagation of three genotypes of Mortiño, Vaccinum meridionale SW., by axilary shoots proliferation
DOI:
https://doi.org/10.17533/udea.acbi.329114Keywords:
acclimatization, axillary shoots, ex vitro rooting, VacciniumAbstract
Mortiño (Vaccinium meridionale Sw.) is an important small fruit specie valuable for its polyphenol content. Effective mass production of plants is needed for establishing commercial plantations. The objective of this research was to develop a system of micropropagation of three wild genotypes of mortiño by means of proliferation of axillary shoots and ex vitro rooting. Mini-cuttings induced through pruning were used as a source of explants for micropropagation. After standardizing the disinfection protocol, the effect of different concentrations of 2 isopenteniladenine (2-iP) was evaluated in the WPM culture medium in the proliferation phase. The rooting was carried out under ex vitro conditions with indolbutíric acid (IBA). In the proliferation stage, a concentration of 5 mg/l of 2-iP was used, although it produced a smaller number of buds (2.1 at 1.7 for explant) than a concentration of 20 mg/l of 2-iP, but they were of axillary origin and higher. Each subculture was carried out every 30 days. These buds took root between 66.6 and 80% under ex vitro conditions with 2.000 mg/l of AIB and they had a 82.7% survival rate. The time elapsed between the rooting of the buds and their transfer to the field was three months.
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