Differentiation of mosquito-pathogenic and nonpathogenic Colombian strains of Bacillus sphaericus by random amplified polymorphic dna
DOI:
https://doi.org/10.17533/udea.acbi.329535Keywords:
Bacillus sphaericus, RAPD, genetic diversityAbstract
RAPD was used to study genetically Colombian strains of Bacillus sphaericus, of which 19 were native stocks pathogenicto the larvae of the mosquito Culex quinquefasciatus, and 37 were non-pathogenic to these larvae. The band patterns weresimilar among the former, which could be differentiated from the strains with no larvacide activity by the 1.800 base pair (bp) band obtained with OPA-08 and the band of 691 bp with OPA-17. The phenogram showed two independent groups with more than 90% dissimilarity, one of which is formed by the genetically similar mosquito-pathogenic strains, and the other comprised of the nonpathogenic strains as a heterogenic group with levels of similarity which varied between 15 and 85%.
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