A SPECTROMETRIC APPROACH TO THE PHENOL TOTALS IN SPECIES OF MELASTOMATACEAEFAMILIY
DOI:
https://doi.org/10.17533/udea.acbi.331558Keywords:
Folin-Ciocalteau, hydrolyzables tannins, Melastomataceae Prussian blueAbstract
This work describes the standardization and evaluation of three analytical methods to quantify total phenolicsin 70% aqueous acetone extracts from twelve species belonging to the Melastomataceae family; a rich sourcein phenolic compounds, mainly hydrolizable tannins. The extracts were evaluated by UV-VIS spectrophotometryusing a direct method (280 nm) and the colorimetric methods of Folin-Ciocalteau (820 nm) and Prussian blue(700 nm), using gallic acid as standard.
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References
Bravo L. 1998. Polyphenols: chemistry, dietary sources,metabolism, and nutritional significance. Nutr. Rev.,56:317-333.
Grubesic RJ, Vukovic J, Kremer D, Vladimir-KnezevicS. 2005. Spectrophotometric method for polyphe-nols analysis: prevalidation and application onPlantago L. species. J. Pharm Biomed Anal.,39:837-842.
Hagerman A. 1998. Modified prussian blue assay fortotal phenolics in tannin chemistry. MiamiUniversity. <http://www.users.muohio.edu/hagermae/tannin.pdf>. Fecha de consulta: Octu-bre 2005.
Haslam E. 1996. Natural polyphenols (vegetable tannins)as drugs: possible modes of action. Journal of Na-tural Products, 59:205-215.
Ikawa M, Schaper TD, Dollard CA, Sasner JJ. 2003.utilization of folin-ciocalteu phenol reagent for thedetection of certain nitrogen compounds. J. Agric.Food Chem., 51:1811-1815.
Isaza JH, Ito H, Yoshida T. 2004. Oligomeric hydrolyzabletannins from Monochaetum multiflorum.Phytochemistry, 65:359-367.
Ito H, Kobayashi E, Li SH, Hatano T, Sugita D, Kubo N,Shimura S, Itoh Y, Tokuda H, Nishino H, Yoshida T.2002. Antitumor activity of compounds isolated fromleaves of Eriobotrya Japonica. J. Agric. FoodChem., 50:2400-2403.
Lamien CE, Meda A, Mans J, Romito M, Nacoulma OG,Viljoen GJ. 2005. Inhibition of fowlpox virus by anaqueous acetone extract from galls of Guiera sene-galensis. J. F. Gmel (Combretaceae). J. Ethnopharma-col., 96:249-253.
Lastra H, RodrÌguez E, Ponce de LeÛn RH, Gonz·lez ML.2000. MÈtodo analÌtico para la cuantificaciÛn detaninos en el extracto acuoso de romerillo. RevistaCubana Plant. Med., 5:17-22.
Latte KP, Kolodziej H. 2000. Antifungal effects of hydroly-sable tannins and related compounds on dermatophy-tes, mould fungi and yeasts. Z Naturforsch [C],55:467-472.
Maldonado PD, Rivero-Cruz I, Mata R, Pedraza-ChaverriJ. 2005. Antioxidant activity of a-type proanthocyani-dins from Geranium niveum (Geraniaceae). J. Agric.Food Chem.,53:1996-2001.
Okuda T. 2005. Systematics and health effects of chemicallydistinct tannins in medicinal plants. Phytochemistry,66:2012-2031.
Yoshida T, Amakura Y, Yokura N, Ito H, Isaza JH, RamÌrezLS, Pel·ez DP, Renner SS, 1999. Oligomerichydrolysable tannins from Tibouchinamultiflora.Phytochemistry, 52:1661-1666.
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