Evaluation of Pap staining and trichrome staining in rumen ciliate protozoa

Authors

  • Richard Zapata Salas University of Antioquia
  • Diana N. Polanco Echeverri University of Antioquia
  • Sandra L. Alzate Uribe University of Antioquia
  • Diana M. Osorno Cano University of Antioquia
  • Isabel C. Martínez Albanés University of Antioquia
  • Paola A. Bedoya Hernández University of Antioquia
  • Lina A. Gutiérrez Builes University of Antioquia
  • Leonardo A. Ríos Osorio University of Antioquia

DOI:

https://doi.org/10.17533/udea.hm.10876

Keywords:

entodiniomorphida, PAP staining, ruminal ciliated protozoa, trichrome staining, vestibulipherida

Abstract

Introduction
Ruminal ciliated protozoa show morphological differences between their orders, including the majority share of its internal structures varying in size, shape and spatial arrangement, which allows differentiation. Therefore, the colors we studied could be a useful tool for morphological identification of these organisms.
ObJective
Evaluate Staining affinity and quality of the Pap staining and Trichrome staining in ruminal ciliated
protozoa.
Materials and methods
Experimental study made from factorial designs analyzed through nonparametric tests. The Pap and Trichrome stains were tested with 36 treatments in triplicate.
Results
For PAP staining in the order Vestibuliferida the preservative formaldehyde 10% (p = 0.040) and time of alcohol exposure to 120 min (p = 0.046) showed statistically significant differences for the affinity; Entodiniomorphida order not present statistically significant in none of the factors, like quality to that color for the two orders. As Trichrome staining, we found that none of the orders had a significant effect of the factors evaluated on the response variable affinity, on the contrary, the quality showed significant differences for the two orders, the order Vestibuliferida showed significant differences using the preservative formalin 10% (p = 0.000) and fixed with Schaudinn 30 min (p = 0.023) and Entodiniomorphida only showed differences with the preservative formalin 10% (p =0.002).
Conclusions
The coloring of the best results in both orders to the two response variables is the Papanicolaou stain.
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Author Biographies

Richard Zapata Salas, University of Antioquia

Professor, School of Microbiology, University of Antioquia.

Diana N. Polanco Echeverri, University of Antioquia

Professor, School of Microbiology, University of Antioquia.

Sandra L. Alzate Uribe, University of Antioquia

Student, School of Microbiology, University of Antioquia. Research Group in Veterinary Microbiology.

Diana M. Osorno Cano, University of Antioquia

Student, School of Microbiology, University of Antioquia. Research Group in Veterinary Microbiology.

Isabel C. Martínez Albanés, University of Antioquia

Student, School of Microbiology, University of Antioquia. Research Group in Veterinary Microbiology.

Paola A. Bedoya Hernández, University of Antioquia

Student, School of Microbiology, University of Antioquia. Research Group in Veterinary Microbiology.

Lina A. Gutiérrez Builes, University of Antioquia

Professor at the School of Microbiology, University of Antioquia.

Leonardo A. Ríos Osorio, University of Antioquia

Professor at the School of Microbiology, University of Antioquia.

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Published

2012-01-17

How to Cite

Zapata Salas, R., Polanco Echeverri, D. N., Alzate Uribe, S. L., Osorno Cano, D. M., Martínez Albanés, I. C., Bedoya Hernández, P. A., Gutiérrez Builes, L. A., & Ríos Osorio, L. A. (2012). Evaluation of Pap staining and trichrome staining in rumen ciliate protozoa. Hechos Microbiológicos, 2(1), 29–39. https://doi.org/10.17533/udea.hm.10876

Issue

Vol. 2 No. 1 (2011)

Section

Artículos de investigación original

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Copyright (c) 2012 Hechos Microbiológicos

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