Transduction methods used on biosensors: amperometry and fluorescence
AbstractBiosensor devices have applications in a variety of fields as environmental analysis, biomedical, bio-defense, food and agriculture. On this kind of sensors, a biological material (known as biomediator) reacts with target analytes and an appropriated transduction system converts that reaction to an electrical signal that can be processed, saved and transmitted by using electronic systems. In this article, two transduction methods used for biosensing applications are described: amperometry that is based on the measurement of the electron transfer occurring inside the biomediator and fluorescence, that is based on the measurement of the re-emitted light. Emphasis has been done on the electronics design, including component selection, useful circuit topologies and common problems and solutions. Electronics has been validated for the development of biosensor-based instruments characterized by low production costs and portability.
A. Turner. Biosensors: Past, present and future. Cranfield University. Available on: http://www.cranfield.ac.uk/health/researchareas/biosensorsdiagnostics/index.html. Accessed: August 28, 2013.
V. Scognamiglio, G. Pezzotti, I. Pezzotti, J. Cano, K. Buonasera, D. Giannini, M. Giardi. “Biosensors for effective environmental and agrifood protection and commercialization: From research to market”. Microchimica Acta. Vol. 170. 2010. pp. 215-225.
S. Mozas, M. Marco, M. Lopez de Alda, D. Barcelo. “Biosensor for environmental application: future development trends-Review”. Pure Appl. Chem. Vol. 76. 2004. pp. 723-752.
K, Buonasera, G. Pezzotti, V. Scognamiglio, A. Tibuzzi, M. Giardi. “New Platform of Biosensors for Prescreening of Pesticide Residues to Support Laboratory Analyses”. Journal of Agricultural and Food Chemistry. Vol. 58. 2010. pp. 5982–5990.
M. Giardi, E. Pace. “Photosynthetic proteins for technological applications”. Trends in Biotechnology. Vol. 3. 2005. pp. 257-263.
G. Rea, G. Pezzottiand, M. Giardi. Portable optical sensor for DNA analysis by means of fluorescent probes Patent RM2006A000683. Registered on December 20th 2006. Available on: https://brevetti.cnr.it/brevetti/download/PDF/datasheet1844.pdf. Accessed: August 28, 2013.
V. Nascimento, L. Angnesio. “Eletrodos fabricados por “silk-screen””. Quím. Nova. Vol. 21. 1998. pp. 614-629.
L. Frenzel. Accurately Measure Nanoampere and Picoampere Currents. Basics of design. A Supplement to Electronic Design. Sponsored by National Instruments. February 15, 2007. Available on: http://electronicdesign.com/test-amp-measurement/accurately-measure-nanoampere-and-picoampere-currents. Accessed: August 28, 2013.
P. Brokaw. An IC Amplifier User’s Guide to Decoupling, Grounding, and Making Things Go Right for a Change. Analog Devices. Application Note (AN202). 2000. Available on: http://www.analog.com/static/imported-files/application_notes/AN-202.pdf. Accessed: August 28, 2013
J. Ardizzoni. A Practical Guide to High-Speed Printed-Circuit-Board Layout. Analog Dialogue. Analog Devices. September 2005. Available on: http://www.analog.com/library/analogdialogue/archives/39-09/layout.pdf. Accessed: August 28, 2013
A. Gopinath. D. Russell. “An Inexpensive Field-Portable Programmable Potentiostat”. The Chemical Educator. Vol. 11. 2006. pp. 23-28.
E. Touloupakis, L. Giannoudi, S. Piletsky, L. Guzzella, F. Pozzoni, M. Giardi. “A multi- biosensor based on immobilized Photosystem II on screen-printed electrodes for the detection of herbicides in river water”. Biosensors and Bioelectronics. Vol. 20. 2005. pp. 1984–1992.
K. Maxwell, G. Johnson. “Chlorophyll fluorescence-a practical guide”. Journal of a Experimental Botany. Vol. 51. 2000. pp. 659-668
D. Rodríguez, S. Carvajal, G. Peñuela. “Uso de biosensores enzimáticos para la detección de analitos en matrices ambientales procedentes de la industria lechera”. Revista Politécnica. N.° 13. 2011. pp. 105-112.
K. Buonasera, M. Lambreva, G. Rea, E. Touloupakis, M. Giardi. “Technological applications of chlorophyll a fluorescence for the assessment of environmental pollutants”. Analytical and Bioanalytical Chemistry. Vol. 401. 2011. pp 1139-1151.
Hamamatsu Photonics. Photodiode Technical Information. Available on: http://sales.hamamatsu.com/assets/applications/SSD/photodiode_technical_information.pdf Accessed: October, 2012
X. Ramus. “Transimpedance Considerations for High-Speed Amplifiers”. Application Report SBOA122. Texas Instruments. 2009. Available on: www.ti.com/general/docs/lit/getliterature.tsp?literatureNumber=sb oa122&fileType=pdf. Accessed: August 28, 2013.
J. Cano, K. Buonasera, G. Pezzotti. “New platform of biosensors based on fluorescence detection for enviromental applications”. Revista Politécnica. N.°13. 2011. pp. 124-132.
L. Govindjee. “Sixty-three Years Since Kautsky: Chlorophyll Fluorescence”. Aust. J. Plant. Physiol. Vol. 22. 1995. pp. 131-160.
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