Transduction methods used on biosensors: amperometry and fluorescence
Keywords:biosensors, amperometry, fluorescence, transduction methods, electronic design
AbstractBiosensor devices have applications in a variety of fields as environmental analysis, biomedical, bio-defense, food and agriculture. On this kind of sensors, a biological material (known as biomediator) reacts with target analytes and an appropriated transduction system converts that reaction to an electrical signal that can be processed, saved and transmitted by using electronic systems. In this article, two transduction methods used for biosensing applications are described: amperometry that is based on the measurement of the electron transfer occurring inside the biomediator and fluorescence, that is based on the measurement of the re-emitted light. Emphasis has been done on the electronics design, including component selection, useful circuit topologies and common problems and solutions. Electronics has been validated for the development of biosensor-based instruments characterized by low production costs and portability.
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