Polymorphism in 1908STR1934 locus of the 3' UTR of the Nramp1 bovine gene in eight cattle breeds


  • Jenny P. González S.
  • Omar A. Saldarriaga
  • Albeiro López Herrera
  • Nelson R. Bermúdez
  • Wildeman Zapata Builes
  • Jorge Ossa Londoño
  • María Teresa Rugeles Lopez
  • Gabriel Bedoya B.




ganado criollo colombiano, microsatÈlite, Nramp1, 3í UTR



The Nramp1 gene has been associated with natural resistance to intracellular microorganisms in several species including bovine. Recent evidence suggests an association between polymorphism in the 3´ untranslated region (3´ UTR) of this gene with resistance/susceptibility (R/S) to Brucella abortus as determined in vivo and in vitro. In this study we tested for the variability of the short tandem repeat (STR) within the 3´ UTR of Nramp1 in six breeds of Colombian creole cattle (CCC) and compared the genotypes with those of Holstein and Brahman, which were recently introduced into this country. In CCC as well as in Holstein we found the allele 175 fixed in all populations. In Brahman, 175 allele was also present with a frequency of 0.467 but additionally, in this breed there appeared five other alleles and among them two previously unreported: 183 y 185; also was found the allele 189 in the Colombian creole Harton del Valle cattle, which is not previously reported. Together these results suggest that the 175 allele in the 3´ UTR Nramp1 may be an ancestral allele in cattle and if this is true the association previously reported with the R/S trait requires further evaluation.

Key words: colombian creole cattle, microsatellite, Nramp1, 3´ UTR.


Análisis de polimorfismos en el locus 1908STR1934 locus del 3´ UTR del gen Nramp1 bovino en ocho razas de ganado El gen Nramp1 ha sido asociado con resistencia natural a microorganismos intracelulares en varias especies incluyendo bovinos. Evidencia reciente sugiere una asociación in vivo e in vitro, entre el polimorfismo en la región 3´ no traducida (3´ UTR del inglés 3´ untranslated region) de este gen con resistencia susceptibilidad (R/S) a Brucella abortus. En este estudio se evaluó la variabilidad de una secuencia repetida en tandem (STR, del inglés short tandem repeat) dentro del 3´ UTR de Nramp1 en seis razas de ganado criollo colombiano (GCC) y se comparó con la variabilidad de los de ese STR en Holstein y Brahman, razas que fueron recientemente introducidas a este país. En GCC así como en Holstein se encontró que el alelo 175 está fijado en todas las poblaciones. En Brahman. El alelo 175estuvo presente con una frecuencia de 0.467, pero adicionalmente en este ganado aparecieron otros 5 alelos, dos de ellos no habían sido previamente reportados: 183 y 185; también se encontró el alelo 189 en el ganado criollo colombiano Hartón del Valle, el cual no está reportado previamente. Estos resultados sugieren que el alelo 175 en el 3´ UTR de Nramp1 puede ser un alelo ancestral en ganado y si esto es cierto, la asociación previamente reportada con la característica de R/S requiere evaluación futura.


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Bedoya G, Carvajal LG, Berm ̇dez NR, Moreno FL, M·rquezME, et al. Estructura molecular y poblacional del ganadocriollo Colombiano. Rev Col Cienc Pec. 2001; 14: 109-120.

Bellamy R, Rawende C, Corrah T, McAdam KPWJ, WhitteHC, et al. Variations in the Nramp1 gene and susceptibilityto tuberculosis in West Africans. N Engl J Med. 1998; 338:640-644.

Blackwell JM, Searle S. Genetic regulation of macrophageactivation: understanding the function of Nramp1 (=Ity /Lsh / Bcg). Immunol Lett. 1999; 65: 73-80.

Carvajal LG, Berm ̇dez NR, Moreno LF, M·rquez ME,Estrada JL, et al. Genetic diversity of colombian criollo cattle.Anim Genet. 1998; 29: 41.47.

Cellier M, Prive G, Belouchi A, Kwan T, Rodrigues V, et al.Nramp1 defines a family of membrane proteins. PNAS. 1995;92: 10089-10093.

Feng J, Li Y, Hashad M, Schurr E, Gros P, et al. W. Bovinenatural resistance associated macrophage protein 1 (Nramp1)gene. Genome Res. 1996; 6: 956-964.

Horin P, Rychlik L, Templeton JW, Adams LG. A complexpattern of microsatellite polymorphism within the bovineNramp1 gene. Eur J Immunogenet. 1999; 26: 311-313.

Hu J, Bumstead N, Barrow P, Sebastiani G, Olien L, et al.Resistance to salmonellosis in the chicken is linked to Nramp1and TNC. Genome Res. 1997; 7: 693-704.

Kuhn DE, Baker BD, Lafuse WP, Zwilling BS. Differentialiron transport into phagosomes isolated from the RAW264.7macrophage cell lines transfected with Nramp1Gly169 orNramp1Asp169. J. Leukoc. Biol. 1999; 66:113-119.

Liu J, Fujiwara M, Buu NT, S·nchez FO, Cellier M, et al.Identification of polymorphisms and sequence variants inthe human homologue of the mouse natural resistance-associated macrophague protein gene. Am J Hum Genet.1995; 56: 845-853.

Machugh DE, Shriver MD, Loftus RT, Cunningham P,Bradley DG. Microsatellite DNA variation and the evolution,domestication and phylogeography of taurine and zebu cattle.(Bos taurus and Bos indicus). Genetics. 1997; 146:1071-1086.

MartÌnez G. The Colombian Criollo Cattle Breeds. In:Proceedings of the third global conference on conservationof domestic animal genetic resources. Rare breedsinternational; Queens University, Kignston, Ontario-Canada. 1995; 161-166.

Mathews GD, Crawford AM. Cloning, sequencing and linkagemapping of the Nramp1 gene of sheep and deer. Anim Genet.1998; 29: 1-6.

Orita M, Suzuki Y, Seliya T, Hayashi K. Rapid and sensitivedetection of point mutations and DNA polymorphims usingthe polymerase chain reaction. Genomics. 1989; 5:874-879.

Price RE, Templeton JW, Smith III R, Adams LG. Ability ofMoncuclear phagocytes from cattle naturally resistant orsusceptible to brucellosis to control in vitro intracelullar survivalof Brucella abortus. Infect Immun. 1990; 58: 879-886.

Qureshi T, Templeton JW, Adams LG. Intracellular survivalof Brucella abortus, Mycobacterium bovis- BCG, Salmonelladublin, and Salmonella typhimurium in macrophages fromcattle genetically resistant to Brucella abortus. Vet. Immun.Immunopat. 1996; 50: 55-65.

Sambrook J, Fritsch EF, Maniatis T. Isolation of high-molecular-weight DNA from mammalian cells. In: SambrookJ, Fritsch EF, Maniatis T., editors. Molecular cloning. Alaboratory manual. New York: Cold Spring Harbor LaboratoryPress; 1989. p. 9.14-9.23.

Swilling BS, Kuhn DE, Wikoff L, Brown D, Lafuse W. Roleof iron in Nramp 1- mediated inhibition of mycobacterialgrowth. Infec Immun. 1999; 67: 1386-1392.

Templeton JW, Adams L.G. Natural resistance to brucellosis:in Advances in Brucellosis Research. Garry Adams ed., 1990:Cap 10.

Tuggle CK, Schmitz CB and Gingerich-Feil D. Cloning of apig Full-length natural resistance associated macrophageprotein (Nramp1) cDNA. J. Anim. Sci. 1997, 75: 277.

Vidal SM, Gross P. Resistance to infection with intracellularparasites: Identification of a candidate gene. NIPS. 1994; 9:178-183.

Vidal SM, Malo D, Vogan K, Skamene E, Gros P. NaturalResistance to infection with intracellular parasites: Isolationof a candidate candidate for BCG. Cell. 1993; 73: 469-485.

Vidal SM, Tremblay ML, Govoni G, Gauthier S, SebastianiG, et al. The Ity/Lsh/Bcg locus: Natural resistance to infectionwith intracellular parasites is abrogated by disruption of theof the Nramp1 gene. J Exp Med. 1995; 182: 655-666.

Vidal SM, Pinner E, Lepage P, Gauthier S, Gros P. Naturalresistance to intracellular infections: Nramp1 encodes amembrane phosphoglycoprotein absent in macrophages fromsusceptible (Nramp1 D169) mouse strains. J Immunol. 1996;157: 3559-3568.




How to Cite

González S., J. P., Saldarriaga, O. A., López Herrera, A., Bermúdez, N. R., Zapata Builes, W., Ossa Londoño, J., Rugeles Lopez, M. T., & Bedoya B., G. (2016). Polymorphism in 1908STR1934 locus of the 3’ UTR of the Nramp1 bovine gene in eight cattle breeds. Revista Colombiana De Ciencias Pecuarias, 19(1), 7. https://doi.org/10.17533/udea.rccp.324028



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