Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs


  • Deny J Zapata Universidad de Antioquia - Medellín
  • Berardo de J Rodríguez
  • María C Ramírez
  • Albeiro López H
  • Jaime Parra S



goblet cells, histochemistry, histopathology,, intestine, pigs



Background: to the best of our knowledge, the effects of lipopolysaccharide (LPS) from Escherichia coli on goblet cells and intestinal mucin secretion of weaned pigs has not been reported, and it is unknown whether these effects could trigger enteritis. Objective: to determine the effect of E. coli LPS on intestinal mucin secretion in weaning piglets. Methods: fifty-two piglets weaned at 21 days of age were fed a basal diet supplemented with four LPS levels (0.0, 0.3, 0.5, and 1.0 μg/mg) during 10 days. Piglets were slaughtered on days 1, 5, 7, and 10 post-weaning and samples of small and large intestine were taken for histochemical staining to determine goblet cell population and type of mucins produced (acidic, sulphated, non-sulphated, or neutral). Results: acidic mucin was reduced on day 5 post-weaning independently of the dietary LPS level supplied to piglets. Recovery of acidic mucins was observed during days 7 and 10 post-weaning. Neutral mucins increased on day 5 and decreased on days 7 and 10 post-weaning. High LPS levels decreased goblet cells population and secretion of all types of mucins. This effect was remarkably high for diet two (D2: 0.5 mg LPS/mg food). Conclusions: early weaning (21 d) and LPS addition to the diet affect mucin secretion and intestinal epithelium integrity by modifying goblet cell populations and their balance between acidic and neutral mucin secretion. These findings explain some abnormalities related with post-weaning diarrhea syndrome and help to explain its pathophysiology.

= 174 veces | PDF
= 142 veces| | HTML
= 14 veces|


Download data is not yet available.


Amador P, García-Herrera J, Marca MC, de la Osada J, Acin S, Navarro MA, Salvador MT, Lostao MP, Rodríguez-Yoldi MJ. Intestinal D-galactose transport in an endotoxemia model in the rabbit. J Membr Biol 2007; 2153:125-133.Bauer E, Metzler-Zebeli BU, Verstegen, MW, Mosenthin R. Intestinal gene expression in pigs: effects of reduced feed intake during weaning and potential impact of dietary components. Nutr Res Rev 2011; 24.2:155-175.Betschera S, Beinekeb A, Schönfelda L, Kamphues J. Effects of diet’s physical form (grinding intensity; meal/pellets) on morphological and histological parameters (e.g. ratio of neutral to acid mucins) of the gastrointestinal tract in weaned piglets. Livest Sci 2010; 134:149-151. Blanco M, Lazo L, Blanco JE, Dahbi G, Mora A, López C, Blanco J. Serotypes, virulence genes, and PFGE patterns of enteropathogenic Escherichia coli isolated from Cuban pigs with diarrhea. Int Microbiol 2010; 9:53-60. Brown DC, Maxwell CV, Erf GF, Davis ME, Singh S, Johnson ZB. The influence of different management systems and age on intestinal morphology, immune cell numbers and mucin production from goblet cells in post-weaning pigs. Vet Immunol Immunopathol 2006; 111:187-198.Deplancke B, Gaskins, HR. Microbial modulation of innate defense: goblet cells and the intestinal mucus layer. Am J Clin Nutr 2012; 73(6):1131S-1141S.Dunsford B, Haensly W, Knabe D. Effects of diet on acidic and neutral goblet cell populations in the small intestine of early weaned pigs. Am J Vet Res 1991; 52:1743-1746.Ghaleb AM, McConnell BB, Kaestner KH, Yang VW. Altered intestinal epithelial homeostasis in mice with intestine-specific deletion of the Krüppel-like factor 4 gene. Dev Biol 2011; 349: 310-320.Gomes N, Brunialti M, Mendes M, Freudenberg M, Galanos C, Salomão R. Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood. Braz J Med Biol Res 2010; 43:853-858.Hedemann MS, Hojsgaard S, Jensen BB. Small intestinal morphology and activity of intestinal peptidases in piglets around weaning. J Anim Physiol Anim Nutr 2003; 87:32-41.

Rev Colomb Cienc Pecu 2015; 28:209-217Zapata DJ et al . E . coli LPS and intestinal mucins in weaned pigsLiu Y, Ipharraguerre IR, Pettigrew JE. Digestive physiology of the pig symposium: Potential applications of knowledge of gut chemosensing in pig production. J Anim Sci 2013; 91:1982-1990.Looft TP. The swine intestinal microbiota: localized adaptations and responses to in-feed antibiotics. Graduate Theses and Dissertations 2012; [Access date: September 13, 2014] URL: MA, Lindén SK, Sutton P, Florin TH. Mucin dynamics and enteric pathogens. Nat Rev Microbiol 2011; 9:265-27.McLamb BL, Gibson AJ, Overman EL, Stahl C, Moeser AJ. Early weaning stress in pigs impairs innate mucosal immune responses to enterotoxigenic E . coli challenge and exacerbates intestinal injury and clinical disease. Plos One 2013; 8(4):e59838. doi:10.1371/journal.pone.0059838Moeser AJ, Borst LB, Overman BL, Pittman JS. Defects in small intestinal epithelial barrier function and morphology associated with peri-weaning failure to thrive syndrome (PFTS) in swine. Res Vet Sci 2012; 93:975-982. Montoya R, Carolina M, López A, Parra S. Alteraciones en la producción mRNA de enzimas intestinales de cerdos durante varios períodos pos-destete. Rev Bio Agro 2012; 10:126-134. Nakamura Y, Hamajima Y, Komori M. The role of Atoh1 in mucous cell metaplasia. International Journal of Otolaryngology 2012; [Access date: September 13, 2014] URL: D, Ciro J, Román Y, Peláez C, López A, Parra J. Cambios en la actividad enzimática en duodeno y yeyuno de cerdos durante varios periodos posdestete. Rev Med Vet Zoot 2011; 58:156-165.Parra J, Agudelo J, Ortiz L, Ramírez MC, Rodríguez B, López A. Lipopolysaccharide (LPS) from Eschericia coli has detrimental effects on the intestinal morphology of weaned pigs. Rev Colomb Cienc Pecu 2011; 24:598-608.Parra J, Agudelo J, Sanín P, Forero J, Muskus C, López A. Intestinal expression of pro-infammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from Escherichia coli in weaned pigs. Rev Colomb Cienc Pecu 2013; 26:108-118. Reis de souza T, Guerrero C, Aguilera B, Mariscal L. Efecto de diferentes cereales sobre la morfología intestinal de lechones recién destetados. Téc Pecu Méx 2005; 43:309-321.Reis de souza T, Guerrero C, Aguilera B, Mariscal L. Cambios nutrimentales en el lechón y desarrollo morfofisiológico de su aparato digestivo. Vet Méx 2012; 43:155-173.Shimizu T, Shimizu S. Differential properties of mucous glycoproteins produced by allergic inflammation and lipopolysaccharide stimulation in rat nasal epithelium. Adv Otorhinolaryngol 2011; 72:107-109. Steel RG, Torrie JH. Principles and procedures of statistics: a biometrical approach. 2nd ed. New York: McGraw-Hill; 1985.Strous GJ, Dekker J. Mucin-type glycoproteins. Crit Rev Biochem Mol Biol 1992; 27:57-92.Vente-Spreeuwenberg MA, Verdonk JM, Bakker GC, Beynen AC, Verstegen MW. Effect of dietary protein source on feed intake and small intestine morphology in newly weaned piglets. Livest Prod Sci 2004; 86:169-177.Voynow JA, Bruce KR. Mucins, mucus and sputum. Chest 2009; 135:505-512.Zhenfeng Z, Deyuan O, Xiangshu P, Sung WK, Yanhong L, Junjun W. Dietary arginine supplementation affects microvascular development in the small intestine of early-weaned pigs. J Nutr 2008; 138:1304-1309.




How to Cite

Zapata, D. J., Rodríguez B. de J., Ramírez, M. C., López H, A., & Parra S, J. (2015). Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs. Revista Colombiana De Ciencias Pecuarias, 28(3), 209–217.



Original research articles

Similar Articles

> >> 

You may also start an advanced similarity search for this article.