Anesthetic use of eugenol and benzocaine in juveniles of red tilapia (Oreochromis sp.)


  • Daniel Santiago Rucinque National University of Colombia
  • Gina Polo University of São Paulo
  • Javier Borbón National University of Colombia
  • Jaime F. González Mantilla National University of Colombia



anesthesia, cortisol, fish, handling, recovery, stress


Background: routine practices such as biometrics, transportation and medical procedures are a source of stress to fish. The use of anesthetics is recommended to reduce undesirable effects of stress, such as decreased feed intake and even mortality -in critical cases. Objective: the aim of this study was to determine the anesthetic efficacy of eugenol and benzocaine in juveniles of red tilapia Oreochromis sp. Methods: thirty juveniles of red tilapia (Oreochromis sp.) were distributed in groups of 10 individuals as follows; G1: eugenol (50 mg/L), G2: eugenol (100 mg/L) and G3: benzocaine (100 mg/L). The fish were individually placed in the corresponding anesthetic solution. After immersion, times to achieve the different anesthetic stages were recorded. During anesthesia, the fish were measured, weighed and blood samples were collected for glucose and plasma cortisol analysis. Results: time to total loss of equilibrium was higher in G3 (p<0.05) compared to the other two groups. No significant differences were observed among groups regarding time to total loss of equilibrium or time to ventilation absence. Glucose levels were significantly higher in G1 (p = 0.03) in comparison to G3. However, cortisol levels were higher in G3 as compared to the other groups (p<0.05). A mortality rate of 10% was observed in G1 fish. Conclusion: current findings indicate that 100 mg/L eugenol leads to induction of anesthesia in less than 3 min and recovery in less than 5 min, without mortality in juveniles of red tilapia.

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Author Biographies

Daniel Santiago Rucinque, National University of Colombia

VM., Research Group in Aquatic & Environmental Toxicology (AQUÁTICA), Laboratory of Aquatic Toxicology, Faculty of Veterinary Medicine and Zootechnics, National University of Colombia, Bogotá, Colombia.

Gina Polo, University of São Paulo

VM., MSc., Laboratory of Epidemiology and Biostatistics, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo, Brazil.

Javier Borbón, National University of Colombia

VM., Aquatic and Environmental Toxicology Research Group (AQUÁTICA), Aquatic Toxicology Laboratory, Faculty of Veterinary Medicine and Zootechnics, National University of Colombia, Bogotá, Colombia.

Jaime F. González Mantilla, National University of Colombia

VM., MSc., PhD., Aquatic and Environmental Toxicology Research Group (AQUÁTICA), Aquatic Toxicology Laboratory, Faculty of Veterinary Medicine and Zootechnics, National University of Colombia, Bogotá, Colombia.


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How to Cite

Rucinque, D. S., Polo, G., Borbón, J., & González Mantilla, J. F. (2017). Anesthetic use of eugenol and benzocaine in juveniles of red tilapia (Oreochromis sp.). Revista Colombiana De Ciencias Pecuarias, 30(1), 60–66.



Original research articles