Frequency of canine degenerative myelopathy SOD1:c.118G>A mutation in 22 dog breeds in Guadalajara, Mexico

Authors

  • Miguel A. Ayala-Valdovinos University of Guadalajara
  • Ana A. Gómez-Fernández University of Guadalajara
  • Theodor Duifhuis-Rivera University of Guadalajara
  • Eva A. Aparicio-Cid University of Guadalajara
  • David R. Sánchez-Chiprés University of Guadalajara
  • Jorge Galindo-García University of Guadalajara

DOI:

https://doi.org/10.17533/udea.rccp.v31n2a08

Keywords:

genetic disease, molecular marker, PCR-RFLP, SOD1 gene

Abstract

Background: Canine degenerative myelopathy (DM) is a late-onset disease that primarily affects large-breed dogs. The disease involves the spinal cord and produces progressive paresia and, eventually, complete loss of mobility. DM has been related to missense mutation c.118G>A in the SOD1 gene. Objective: To determine the genotypic and genic frequencies of DM in Mexico. Methods: In total, 330 samples from 22 different dog breeds were genotyped using the polymerase chain reaction and restriction fragment length polymorphisms (PCR-RFLP) technique. Results: The mutation was identified in 71 animals from 11 different breeds. Observed genic frequencies were 0.78 for the G allele and 0.14 for the A allele. Genotypic frequencies were 0.79 for the G/G wild-type, 0.14 for the G/A heterozygote, and 0.7 for the A/A homozygote. Conclusion: The genic frequency of this allele is high among the studied populations. A molecular marker program that identifies the DM mutation in breeding dogs should be implemented in order to reduce this frequency.

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Author Biographies

Miguel A. Ayala-Valdovinos, University of Guadalajara

Zoot, VM, MSc, PhD., Department of Animal Production, University Center for Biological and Agricultural Sciences, University of Guadalajara, Mexico.

Ana A. Gómez-Fernández, University of Guadalajara

IS., Department of Animal Production, University Center for Biological and Agricultural Sciences, University of Guadalajara, Mexico.

Theodor Duifhuis-Rivera, University of Guadalajara

Zoot, VM, MSc., Department of Animal Production, University Center for Biological and Agricultural Sciences, University of Guadalajara, Mexico.

Eva A. Aparicio-Cid, University of Guadalajara

Zoot, VM., Department of Animal Production, University Center for Biological and Agricultural Sciences, University of Guadalajara, Mexico.

David R. Sánchez-Chiprés, University of Guadalajara

Zoot, VM, MSc, PhD., Department of Animal Production, University Center for Biological and Agricultural Sciences, University of Guadalajara, Mexico.

Jorge Galindo-García, University of Guadalajara

Zoot, VM, MSc, PhD., Department of Animal Production, University Center for Biological and Agricultural Sciences, University of Guadalajara, Mexico.

References

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Published

2018-06-20

How to Cite

Ayala-Valdovinos, M. A., Gómez-Fernández, A. A., Duifhuis-Rivera, T., Aparicio-Cid, E. A., Sánchez-Chiprés, D. R., & Galindo-García, J. (2018). Frequency of canine degenerative myelopathy SOD1:c.118G>A mutation in 22 dog breeds in Guadalajara, Mexico. Revista Colombiana De Ciencias Pecuarias, 31(2), 150–154. https://doi.org/10.17533/udea.rccp.v31n2a08

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