Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method


  • Roger C. Maia State University of Northern Rio de Janeiro Darcy Ribeiro
  • Edgar M. Mogollón Waltero Cooperative University of Colombia
  • Diego F. Dubeibe Marin Cooperative University of Colombia
  • Bruno Pena Carvalho Brazilian Agricultural Research Corporation
  • Ángelo J. Burla Dias State University of Northern Rio de Janeiro Darcy Ribeiro




colorimetric test, cumulus-oophorus complexes, in vitro maturation, pasture, seasons


Background: The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. This makes it a useful tool to select best-quality oocytes at the times of the year when there is forage restriction. Objective: To evaluate the effect of seasonality on the nuclear maturation and quality of oocytes selected by the BCB test. Methods: The cumulus-oophorus complexes (COCs) were obtained in summer and winter of 2010 and 2011. Selected COCs were maintained for 90 min at 38.5 °C in a CO2 incubator, in TCM 199 medium containing 10% fetal bovine serum and antibiotics, and supplemented with 26 μM brilliant cresyl blue. Afterwards, they were divided according to the ooplasm staining (BCB+ —blue; BCB− —unstained). Subsequently, COCs were matured for 22 h. Nuclear maturation was evaluated at 22 h of culture. Results: The proportion of BCB− oocytes was higher in the winter of 2010, but there was no increase in this group in the winter of 2011. The percentage of oocytes that reached metaphase II was higher in control and BCB+ groups in relation to oocytes from BCB− group. Conclusion: The season of the year influences the percentage of oocytes best suited for embryonic production in situations in which oocyte donors receive pasture-based feeding, since the method was effective in determining the effect of seasonality on the competence of bovine oocytes to reach nuclear maturation.

= 264 veces | PDF
= 155 veces|


Download data is not yet available.

Author Biographies

Roger C. Maia, State University of Northern Rio de Janeiro Darcy Ribeiro

VM, MSc., Animal Reproduction and Genetic Improvement Laboratory, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Brazil.

Edgar M. Mogollón Waltero, Cooperative University of Colombia

VM, PhD., Animal Reproduction Biotechnology Laboratory, Cooperative University of Colombia, Bucaramanga, Colombia.

Diego F. Dubeibe Marin, Cooperative University of Colombia

VM, MSc., Animal Reproduction Biotechnology Laboratory, Cooperative University of Colombia, Bucaramanga, Colombia.

Bruno Pena Carvalho, Brazilian Agricultural Research Corporation

MSc, DrSc., Technology Transfer Programming Implementation Sector, Brazilian Agricultural Research Corporation (EMBRAPA), Rio Branco, Brazil.

Ángelo J. Burla Dias, State University of Northern Rio de Janeiro Darcy Ribeiro

VM, PhD., Animal Reproduction and Genetic Improvement Laboratory, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Brazil.


Adamiak SJ, Mackie K, Watt RG, Webb R, Sinclair KD. Impact of nutrition on oocyte quality: Cumulative effects of body composition and diet leading to hyperinsulinemia in cattle. Biol Reprod 2005; 73:918-926.

Alm H, Torner H, Lohrke V, Ghone IM, Kanitz W. Bovine blastocyst development rate in vitro is influenced by selection of by brilliant cresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity. Theriogenology 2005; 63: 2194-2205.

Alvares CA, Stape JL, Sentelhas PC, Gonçalves JLM, Sparovek G. Köppen’s climate classification map for Brazil. Meteorologische Zeitschrift 2013; 22(6):711-728.

Ashry M, Lee K, Mondal M, Datta TK, Folger JK, Rajput SK, Zhang K, Hemeida NA, Smith G. Expression of TGFβ superfamily components and other markers of oocyte quality in oocytes selected by brilliant cresyl blue staining: Relevanceto early embryonic development. Mol Repro Dev 2015; 82(3):251-264.

Castaneda CA, Kaye P, Pantaleon M, Phillips NC, Norman S, Fry R, D’occhio M. Lipid content, active mitochondria and brilliant cresyl blue staining in bovine oocytes. Theriogenology 2013; 79:417-422.

De Loos F, Van Vliet C, Van Maurick P, Kruip TA. Morphology of immature bovine oocytes. Gamete Res 1989; 24:197-204.

Euclides VPB, Macedo MCM, Zimmer AH, Medeiros RN, Oliveira MP. Características do pasto de capim-tanzânia adubadocom nitrogênio no final do verão. Pesq Agropec Bras 2007; 42(8):1189-1198.

Emidio ZPO. Estações do Ano. Ipmet 2016; [Access date: May 10th, 2017]. URL: https://www.ipmet.unesp.br/4estacoes/#Ferreira AM. Nutrição e atividade ovariana em bovinos: Uma revisão. Pesq Agropec Bras 1993; 28:1077-1093.

Fulka JJr, First NL, Moor RM. Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation. Mol Hum Reprod 1998; 4:41-49.

Goovaerts IGF, Leroy JLMR, Jorsen EPA, Bols PEJ. Non invasivebovine oocyte quality assessment: Possibilities of a single oocyte culture. Theriogenology 2010; 74:1509-1520.

Hansen PJ. Physiological and cellular adaptations of zebu cattle to thermal stress. Anim Reprod Sci 2004; 82:349-360.

Lonergan P, Fair T. Maturation of oocyte in vitro. Annu Rev Anim Biosci 2016. 4:255-268.

Manjunatha BM, Gupta PSP, Devaraj M, Ravindra JP, Nandi S. Selection of developmentally competent buffalo oocytes by brilliant cresyl blue staining before IVM. Theriogenology 2007; 68:1299-1304.

Mirshamsi SM, Karamishabankareh H, AhmadI-Hamedani M, Soltani L, Hajarian H, Abdolmohammadi AR. Combination of oocyte and zygote selection by brilliant cresyl blue (BCB) test enhanced prediction of developmental potential to the blastocyst in cattle. Anim Reprod Sci 2013; 136:245-251.

Mota GB, Batista RITP, Serapião RV, Boité MC. Developmental competence and expression of MATER and ZAR1 gene in immature bovine oocytes selected by brilliant cresyl blue. Zygote 2009; 26:1-8.

Nicoloso RS, Lanzanova ME, Lovato T. Manejo das pastagens de inverno e potencial produtivo de sistemas de integração lavoura-pecuária no estado do Rio Grande do Sul. Cienc Rural 2006; 36(6):1799-1805.

Paula-Lopes FF, Chase CC JR, Al-Katanani YM, Krininger CE, Rivera RM, Tekin S, Majewski AC, Ocon OM, Olson TA, HansenPJ. Genetic divergence in cellular resistance to heat shock in cattle: Differences between breeds developed in temperate versus hot climates in responses of preimplantation embryos, reproductive tissues and lymphocytes to increase culture temperatures. Reprod 2003; 125:285-294.

Mendonça JC, Freitas RM, Shimabukuro YE, Marques VS. Avaliação de eventos de inundação na Região Norte Fluminense, Rio de Janeiro, utilizando imagens de sensores remotos. Ambi-Água 2012; 7:255-267.

O’Shea LC, Mehta J, Lonergan P, Hensey C, Fair T. Developmental competence in oocytes and cumulus cells: Candidate genes and networks. Syst Biol Reprod Med 2012, 58:88-101.

Rodríguez-Gonzalez E, Lopez-Bejar M, Velilla E, Paramio MT. Selection of prepuberal goat oocytes using the brilliant cresyl blue test. Theriogenology 2002; 57:1397-1409.

Rodrigues CFM. Historical context of cattle embryo transfer technique in Brazil. Anim Reprod 2014; 11(3):137-140.

Sales JN, Iguma LT, Batista RI, Quintão CC, Gama MA, Freitas C, Pereira C, Camargo LS, Viana JH, Souza JC, Baruselli PS. Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos Taurus cows. J Dairy Sci 2015; 98:3086-3099.

Sansigolo CA, Kayano MT. Trends of seasonal maximum and minimum temperatures and precipitations in Southern Brazil for de 1913-2006 period. Theor Appl Climatol 2010; 101:209-216.

Santos J, Cerri R, Sartori R. Nutritional management of the donor cow. Theriogenology 2008; 69:88-97.

Sartori R, Guardieiro MM. Fatores nutricionais associados à reprodução da fêmea bovina. R Bras Zootec 2010; 39:422-432.

Smitz JEJ, Nogueira D, Vanhoutte Matos DG, Cortvrindt RN. Oocyte: In vitro maturation. In: Suh CS, Sonntag B, Erickson GF. The ovarian life cycle: A contemporany view. Rev Endocr Metab Disord 2004; 3:5-12.

Teixeira FA, Bonomo P, Pires AJV, Silva FF, Fries DD, Hora DD. Produção anual e qualidade de pastagem de Brachiaria decumbens diferida e estratégias de adubação nitrogenada. Acta Sci Anim Sci 2011; 33:241-248.

Van Den Hurk R, Zhao J. Formation of mammalian oocytes and their growth, differentiation and maturation within ovarian follicles. Theriogenology 2004; 63:1717-1751.

Wolfenson D, Roth Z, Meidan R. Impaired reproduction in heat stressed cattle: Basic and applied aspects. Anim Reprod Sci 2000; 60:535-547.

Wongsrikeao P, Otoi T, Yamasaki H, Agung B, Taniguchi M, Naoi H, Shimizu R, Nagai T. Effects of single and double exposure to brilliant cresyl blue on the selection of porcine oocytes for in vitro production of embryos. Theriogenology 2006; 66:366-372.

Wu Y-G, Liu Y, Zhou P, Lan G-C, Han D, Miao D-Q, Tan J-H. Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: A study using the mouse model. Cell Res 2007; 17:722-731.

Zeron Y, Ocheretny A, Kedar O, Borochov A, Sklan D, Arav A. Seasonal changes in bovine fertility: Relation to oocyte developmental competence, membrane properties and follicle fatty acid composition. Reprod 2001; 121:447-454.




How to Cite

Maia, R. C., Mogollón Waltero, E. M., Dubeibe Marin, D. F., Pena Carvalho, B., & Burla Dias, Ángelo J. (2017). Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method. Revista Colombiana De Ciencias Pecuarias, 30(4), 259–266. https://doi.org/10.17533/udea.rccp.v30n4a01



Original research articles

Similar Articles

> >> 

You may also start an advanced similarity search for this article.