Genetic diversity in oocyte donors used in in vitro bovine embryo production programs in Brazil

Authors

  • Wilder Hernando Ortiz Vega State University of Northern Rio de Janeiro Darcy Ribeiro
  • Celia Raquel Quirino State University of Northern Rio de Janeiro Darcy Ribeiro
  • Aylton Bartholazzi Junior State University of Northern Rio de Janeiro Darcy Ribeiro
  • Clara Slade Oliveira Embrapa Dairy Cattle
  • Raquel Varella Serapião Agricultural Research Company of Rio de Janeiro State
  • Caroline Marçal Gomes David State University of Northern Rio de Janeiro Darcy Ribeiro
  • Miguel Alejandro Silva Rua State University of Northern Rio de Janeiro Darcy Ribeiro
  • Ana Carolina Barros de Freitas State University of Northern Rio de Janeiro Darcy Ribeiro

DOI:

https://doi.org/10.17533/udea.rccp.v32n2a02

Keywords:

allele frequencies, heterozygosity, inbreeding, microsatellite markers, oocyte

Abstract

Background: Current reproductive management of bovine elite populations involves the use of assisted reproductive technologies (ARTs), aiming to obtain the greatest genetic gain. However, inadequate use of ARTs may lead to loss of genetic diversity in the offspring. Objective: To assess the genetic diversity in elite female cattle populations used in commercial in vitro embryo production. Methods: Using genetic and ecological approaches for the study of populations based on microsatellite markers, we assessed the genetic diversity between and within populations of cows used in commercial in vitro embryo production programs in Brazil. Results: Endogamy within populations varied from zero to 9.1%, while heterozygosity between populations (FST) was <0.05 in the different population interactions. AMOVA showed 1% variation between populations, 8% between individuals and 91% within individuals. The dimensionality reduction method utilized indicated a lack of structure in the populations analyzed, identifying two main clusters in the three populations. Conclusions: Low genetic diversity between cow populations associated with commercial programs of in vitro embryo production in Brazil was evidenced. Variable levels of endogamy within the populations were observed. Approaches of population genetics as well as ecological diversity can be implemented to more thoroughly estimate genetic diversity in livestock populations.

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Author Biographies

Wilder Hernando Ortiz Vega, State University of Northern Rio de Janeiro Darcy Ribeiro

VMZ, PhD., Laboratory of Animal Reproduction and Genetic Improvement, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro.

Celia Raquel Quirino, State University of Northern Rio de Janeiro Darcy Ribeiro

Agr. Eng., PhD., Laboratory of Animal Reproduction and Genetic Improvement, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.

 

Aylton Bartholazzi Junior, State University of Northern Rio de Janeiro Darcy Ribeiro

VM, PhD., Laboratory of Animal Reproduction and Genetic Improvement, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.

Clara Slade Oliveira, Embrapa Dairy Cattle

VM, PhD., Embrapa Dairy Cattle, Animal Reproduction Laboratory, Santa Mônica Experimental Field (CESM), Valença, Rio de Janeiro. Brazil.

Raquel Varella Serapião, Agricultural Research Company of Rio de Janeiro State

VM, PhD., Agricultural Research Corporation of the State of Rio de Janeiro (Pesagro-Rio), Animal Reproduction Laboratory, Santa Mônica Experimental Field (CESM), Valença, Rio de Janeiro. Brazil.

Caroline Marçal Gomes David, State University of Northern Rio de Janeiro Darcy Ribeiro

VM, PhD., Laboratory of Animal Reproduction and Genetic Improvement, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.

 

Miguel Alejandro Silva Rua, State University of Northern Rio de Janeiro Darcy Ribeiro

VM, PhD., Laboratory of Animal Reproduction and Genetic Improvement, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.

Ana Carolina Barros de Freitas, State University of Northern Rio de Janeiro Darcy Ribeiro

VM, PhD., Laboratory of Animal Reproduction and Genetic Improvement, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.

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Published

2019-05-17

How to Cite

Ortiz Vega, W. H., Quirino, C. R., Bartholazzi Junior, A., Slade Oliveira, C., Varella Serapião, R., Gomes David, C. M., Silva Rua, M. A., & Barros de Freitas, A. C. (2019). Genetic diversity in oocyte donors used in in vitro bovine embryo production programs in Brazil. Revista Colombiana De Ciencias Pecuarias, 32(2), 90–99. https://doi.org/10.17533/udea.rccp.v32n2a02

Issue

Vol. 32 No. 2 (2019): April - June 2019

Section

Original research articles

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