Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae

Keywords: antimicrobial peptide, aquaculture, biofloc, gene expression, Penaeidin, RAS, shrimp, shrimp immune system

Abstract

Background: Shrimp farming is evolving from semi-intensive to hyper-intensive systems with biofloc technology and water recirculation systems. Objective: To evaluate the transcriptional response promoted by biofloc on shrimp (Litopenaeus vannamei) under a recirculating aquaculture system (RAS). Methods: Quantitative real-time RT-PCR was used to monitor seven key genes related to the immune system in shrimp post-larvae, reared in a RAS with and without biofloc (BF and noBF). In addition, we present for the first time nucleotide sequences of ADP-ribosylation factor 4 (LvArf4) from Litopenaeus vannamei. Results: Transcripts for penaeidin3 (Pen3), penaeidin4 (Pen4), crustin, and Toll receptor (LvToll) genes were upregulated between 3 and 24 h in both systems, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in no-BF as an early response. Regarding differential expression between treatments, 13 occurrences were encountered. Nine that were higher in BF than in no-BF and four higher in no-BF than in BF. In some sample times, expression of Pen3, crustin, LvToll, TRAF6, IMD, and LvArf4 was higher in BF than in no-BF and in others, expression of Pen3, Pen4, and TRAF6 was higher in no-BF than in BF. Conclusions: BF modulates the transcription of genes related to the immune response in shrimp as an early response. However, the RAS with no-BF promotes a similar response.

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Author Biographies

Jorge Soto Alcalá, National Polytechnic Institute, Autonomous University of Sinaloa

National Polytechnic Institute, Interdisciplinary Research Center for Integral Regional Development, Sinaloa Unit (CIIDIR Sinaloa), Guasave, Sinaloa, México. Autonomous University of Sinaloa, Faculty of Marine Sciences, Mazatlán, Sinaloa, México.

Píndaro Álvarez Ruiz, National Polytechnic Institute

National Polytechnic Institute, Interdisciplinary Research Center for Regional Integral Development, Sinaloa Unit (CIIDIR-Sinaloa), Guasave, Sinaloa, México.

Juan M. Audelo Naranjo, Autonomous University of Sinaloa

Autonomous University of Sinaloa. Faculty of Marine Sciences, Mazatlán, Sinaloa, México.

Héctor M. Esparza Leal, National Polytechnic Institute

National Polytechnic Institute, Interdisciplinary Research Center for Regional Integral Development, Sinaloa Unit (CIIDIR-Sinaloa), Guasave, Sinaloa, México.

Irasema E. Luis Villaseñor, Autonomous University of Sinaloa

Autonomous University of Sinaloa, Faculty of Marine Sciences, Mazatlán, Sinaloa, México.

José A. Estrada-Godínez, Autonomous University of Sinaloa

Autonomous University of Sinaloa, Faculty of Marine Sciences, Mazatlán, Sinaloa, México.

Antonio Luna-González, National Polytechnic Institute

National Polytechnic Institute, Interdisciplinary Research Center for Regional Integral Development, Sinaloa Unit (CIIDIR-Sinaloa), Guasave, Sinaloa, México.

Carina Gámez-Jiménez, National Polytechnic Institute

National Polytechnic Institute, Interdisciplinary Research Center for Regional Integral Development, Sinaloa Unit (CIIDIR-Sinaloa), Guasave, Sinaloa, México.

Genaro Diarte Plata, National Polytechnic Institute

National Polytechnic Institute, Interdisciplinary Research Center for Regional Integral Development, Sinaloa Unit (CIIDIR-Sinaloa), Guasave, Sinaloa, México.

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Published
2020-01-31
How to Cite
Soto Alcalá J., Álvarez Ruiz P., Audelo Naranjo J. M., Esparza Leal H. M., Luis Villaseñor I. E., Estrada-Godínez J. A., Luna-González A., Gámez-Jiménez C., & Diarte Plata G. (2020). Comparing RAS with and without biofloc: transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae. Revista Colombiana De Ciencias Pecuarias, 33(1), 32-43. https://doi.org/10.17533/udea.rccp.v33n1a03
Section
Original research articles