Molecular characterization of bacterial microbiota associated with infectious bovine keratoconjunctivitis in Michoacán, Mexico

Authors

  • Ana M. Ríos-Alanís Michoacan University of Saint Nicholas of Hidalgo
  • Joel E. López-Meza Michoacan University of Saint Nicholas of Hidalgo
  • Alejandra Ochoa-Zarzosa Michoacan University of Saint Nicholas of Hidalgo
  • Jose C. Segura-Correa Autonomous University of Yucatán
  • José Herrera-Camacho Michoacan University of Saint Nicholas of Hidalgo

DOI:

https://doi.org/10.17533/udea.rccp.v34n1a02

Keywords:

bacteria, bovine, corneal ulceration, dairy cattle, eye infection, IBK, keratoconjunctivitis, Moraxella bovis, Moraxella bovoculi, ocular bacteria, ocular disease, pink eye

Abstract


Background: The most common ocular disease affecting cattle worldwide is infectious bovine keratoconjunctivitis (IBK), which has been associated with Moraxella bovis bacterium. Objective: To report the molecular characterization of the ocular bacterial microbiota and its relation to IBK in cattle in two dairy regions in Michoacán, Mexico. Methods: A total population of 761 bovines were evaluated, of which 17 (2.23%) showed symptoms of IBK. Thirty-eight bacterial isolates from ocular samples of bovines with IBK were characterized by Gram-staining and antimicrobial sensitivity. In addition, isolates were identified by sequence comparisons of the 16S ribosomal gene. Results: The genus Moraxella was one of the most abundant bacteria and M. bovoculi was the most predominant species. Conclusion: The bacterial isolates identified in eye lesions of cattle and associated to IBK are diverse. To the author´s knowledge, this is the first study on the subject in Mexico; therefore, more research is needed to estimate the incidence of IBK and determine its associated microbiota.

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Author Biographies

Ana M. Ríos-Alanís, Michoacan University of Saint Nicholas of Hidalgo

https://orcid.org/0000-0002-2345-9637
Institute of Agricultural and Forestry Research, Michoacan University of Saint Nicholas of Hidalgo, Morelia, Michoacán, México.

Joel E. López-Meza, Michoacan University of Saint Nicholas of Hidalgo

https://orcid.org/0000-0002-3269-9202
Multidisciplinary Center for Studies in Biotechnology, Faculty of Veterinary Medicine and Zootechnics, Michoacan University of Saint Nicholas of Hidalgo, Morelia, Michoacán, México.

Alejandra Ochoa-Zarzosa, Michoacan University of Saint Nicholas of Hidalgo

https://orcid.org/0000-0003-3441-2989
Multidisciplinary Center for Studies in Biotechnology, Faculty of Veterinary Medicine and Zootechnics, Michoacan University of Saint Nicholas of Hidalgo, Morelia, Michoacán, México.

Jose C. Segura-Correa, Autonomous University of Yucatán

https://orcid.org/0000-0003-1329-9948
Faculty of Veterinary Medicine and Zootechnics, Autonomous University of Yucatán, Mérida, Yucatán, México.

José Herrera-Camacho, Michoacan University of Saint Nicholas of Hidalgo

https://orcid.org/0000-0002-0207-3313
Institute of Agricultural and Forestry Research, Michoacan University of Saint Nicholas of Hidalgo, Morelia, Michoacán, México.

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Published

2020-06-10

How to Cite

Ríos-Alanís, A. M., López-Meza, J. E., Ochoa-Zarzosa, A., Segura-Correa, J. C., & Herrera-Camacho, J. (2020). Molecular characterization of bacterial microbiota associated with infectious bovine keratoconjunctivitis in Michoacán, Mexico. Revista Colombiana De Ciencias Pecuarias, 34(1), 18–28. https://doi.org/10.17533/udea.rccp.v34n1a02

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