Molecular characterization of bacterial microbiota associated with infectious bovine keratoconjunctivitis in Michoacán, Mexico

  • Ana M. Ríos-Alanís Michoacan University of Saint Nicholas of Hidalgo
  • Joel E. López-Meza Michoacan University of Saint Nicholas of Hidalgo
  • Alejandra Ochoa-Zarzosa Michoacan University of Saint Nicholas of Hidalgo
  • Jose C. Segura-Correa Autonomous University of Yucatán
  • José Herrera-Camacho Michoacan University of Saint Nicholas of Hidalgo
Keywords: bacteria, bovine, corneal ulceration, dairy cattle, eye infection, IBK, keratoconjunctivitis, Moraxella bovis, Moraxella bovoculi, ocular bacteria, ocular disease, pink eye


Background: The most common ocular disease affecting cattle worldwide is infectious bovine keratoconjunctivitis (IBK), which has been associated with Moraxella bovis bacterium. Objective: To report the molecular characterization of the ocular bacterial microbiota and its relation to IBK in cattle in two dairy regions in Michoacán, Mexico. Methods: A total population of 761 bovines were evaluated, of which 17 (2.23%) showed symptoms of IBK. Thirty-eight bacterial isolates from ocular samples of bovines with IBK were characterized by Gram-staining and antimicrobial sensitivity. In addition, isolates were identified by sequence comparisons of the 16S ribosomal gene. Results: The genus Moraxella was one of the most abundant bacteria and M. bovoculi was the most predominant species. Conclusion: The bacterial isolates identified in eye lesions of cattle and associated to IBK are diverse. To the author´s knowledge, this is the first study on the subject in Mexico; therefore, more research is needed to estimate the incidence of IBK and determine its associated microbiota.

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Author Biographies

Ana M. Ríos-Alanís, Michoacan University of Saint Nicholas of Hidalgo
Institute of Agricultural and Forestry Research, Michoacan University of Saint Nicholas of Hidalgo, Morelia, Michoacán, México.

Joel E. López-Meza, Michoacan University of Saint Nicholas of Hidalgo
Multidisciplinary Center for Studies in Biotechnology, Faculty of Veterinary Medicine and Zootechnics, Michoacan University of Saint Nicholas of Hidalgo, Morelia, Michoacán, México.

Alejandra Ochoa-Zarzosa, Michoacan University of Saint Nicholas of Hidalgo
Multidisciplinary Center for Studies in Biotechnology, Faculty of Veterinary Medicine and Zootechnics, Michoacan University of Saint Nicholas of Hidalgo, Morelia, Michoacán, México.

Jose C. Segura-Correa, Autonomous University of Yucatán
Faculty of Veterinary Medicine and Zootechnics, Autonomous University of Yucatán, Mérida, Yucatán, Mexico.

José Herrera-Camacho, Michoacan University of Saint Nicholas of Hidalgo
Instituto de Investigaciones Agropecuarias y Forestales. Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.


Addison B. Research shows new emerging strain of pinkeye. Progressive Cattleman 2011; 5:22–23.

Alexander D. Infectious bovine kerato-conjunctivitis: A review of cases in clinical practice. Vet Clin Food Anim 2010; 26:487–503. DOI:https://doi:10.1016/j.cvfa.2010.09.006

Angelos JA, Spinks PQ, Ball LM, George LW. Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis. Int J Syst Evol Microbiol 2007; 57:789–795. DOI:

Blood DC, Radostits OM. Enfermedades causadas por bacterias. En: Medicina Veterinaria. Séptima edición. Vol I. Madrid Interamericana. McGraw-Hill, 1992; 750–753.

Bradford P. A. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbol Rev 2001; 14:933–951. DOI:

Gasque R. Queratoconjuntivitis infecciosa bovina. Enciclopedia Bovina. Enfermedades de los bovinos. Facultad de Medicina Veterinaria y Zootecnia-UNAM. 2008. Fecha de consulta: 12 de enero 2015.

Handool H. Isolation and identification of some genera and species of bacteria and fungi from conjunctiva in cattle in Al-Diwaniya city. AL-Qadisiya J Vet Med Sci 2013; 12:55–62.DOI:

Hare WR, Hoyt PG, Hohn C, Higgins JA. Ribosomal RNA-based analysis of the bacterial flora from the conjunctivae of cattle with bovine keratoconjunctivitis (BKC). Vet Microbiol 2008; 131:358–368. DOI:

Hernández P, Quintero G. Etiología bacteriana de infecciones oculares externas. Nova Publicación Científica 2003; 1:57–64. DOI:

Infante FM, Flores G, Falcón NA, Infante AF. Control de un brote de queratoconjuntivitis infecciosa bovina mediante el control de las moscas en estadio larvario. Med Vet 2000; 17:273–276. DOI: .21258299.1286023369.1582899306-648624553.1559616002

Kowalski AP, Maboni G, Gressler LT, Espíndola JP, Balzan C, Tasca C, Guizzo JA, Conceição F R, Frandoloso R, de Vargas AC. Antigenic characterization of Moraxella bovis, Moraxella bovoculi and Moraxella ovis strains with potential use in vaccines. Vet Microbiol 2017; 210:56–63. DOI:

Libardoni F, Scherer Ch, Farias L, Vielmo A, Balzan C, Vargas A. Moraxella bovoculi in cases of infectious bovine keratoconjunctivitis in Rio Grande do Sul. Brazil. Pesq Vet Bras 2007; 32:743–746. DOI:

Loy JD, Brodersen BW. Moraxella spp. isolated from field outbreaks of infectious bovine keratoconjunctivitis: a retrospective study of case submissions from 2010 to 2013. J Vet Diag Invest 2014; 26:761–768. DOI:

NCBI (National Center for Biotechnology Information). Fecha de consulta: diciembre 2016.

Minas K, McEwan N, Newbold CJ, Scott KP. Optimization of a high-throughput CTAB-based protocol for the extraction of qPCR-grade DNA from rumen fluid, plant and bacterial pure cultures, FEMS Microbiology Letters 2011; 325 (2)2:162–169. DOI:

Navarro JM, Tormo A, Martínez-García E. Stationary phase in gram-negative bacteria, FEMS Microbiol Rev 2010; 34:476–495. DOI:

Ochoa A, Loeza P, Torres F, Loeza H, Mascot N, Sánchez S, López JE. Antimicrobial susceptibility and invasive ability of Staphylococcus aureusisolates from mastitis from dairy backyard system. Antonie Leeuwenhoek 2008; 94:199–206.DOI:

Russell A. Bacterial outer membrane and cell wall penetration and cell destruction by polluting chemical agents and physical conditions. Sci Prog 2003; 86(4):283–311. DOI:

Schnee C, Heller M, Schubert E, Sachse K.Point prevalence of infection withMycoplasma bovoculi and Moraxella spp. incattle at different stages of infectious bovinekeratoconjunctivitis. Vet J 2015; 203:92–96. DOI:

Sosa V, Zunino P. Diversity of Moraxellaspp. Strains recovered from infectious bovine Keratoconjunctivitis cases in Uruguay. J Infect Dev Ctries 2013; 7:819–824. DOI:

Spradbrow PB. A microbiological study of bovine conjunctivitis and Keratoconjunctivitis. Aus Vet J 1967; 43:55–58. DOI:

Takele G, Zerihun A. Epidemiology of infectious keratoconjunctivitis in cattle in South-east Ethiopia. J. Vet. Med. A 2000; 47:169–173. DOI:

Wilcox GE. Bacterial flora of the bovine eye with special reference to the Moraxella and Neisseria. Aust Vet J 1970; 46:253–256. DOI:

Williams DL. Welfare issues in farm animal ophthalmology. Veterinary Clinics of North America: Food Anim Prac 2010; 26:427–435. DOI:

Zamora QMA, Aguilar AJ, Sumano L. Eficacia clínica del florfenicol oftalmico vs florfenicol parenteral en el tratamiento de queratoconjuntivitis infecciosa bovina. Vet Méx 2010; 41:219–25. DOI:

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