Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells

Authors

  • Pablo E. Ocampo São Paulo State University
  • Viviana Vallejo São Paulo State University
  • Luis M. Montoya National University of Colombia
  • Noeme S. Rocha São Paulo State University
  • Fernanda da C. Landim São Paulo State University
  • Sheila C. Rahal São Paulo State University

DOI:

https://doi.org/10.17533/udea.rccp.v34n3a06

Keywords:

cell differentiation, cartilage, chondrocytes, chondrogenesis, chondrogenic differentiation, collagen, corticosteroids, glucocorticoid, histology, hyaluronic acid, mesenchymal stem cell, osteoarthritis, triancinolone acetonide, viscosupplementation

Abstract

Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.

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Author Biographies

Pablo E. Ocampo, São Paulo State University

https://orcid.org/0000-0001-8504-5926
São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

Viviana Vallejo, São Paulo State University

https://orcid.org/0000-0002-6270-261X
São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

Luis M. Montoya, National University of Colombia

https://orcid.org/0000-0002-5198-7998
Faculty of Veterinary Medicine, National University of Colombia, Bogotá, Colombia. Research Group in Veterinary Pathology.

Noeme S. Rocha, São Paulo State University

https://orcid.org/0000-0002-8188-8149
São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

Fernanda da C. Landim, São Paulo State University

http://orcid.org/0000-0002-2420-2550
São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

Sheila C. Rahal, São Paulo State University

http://orcid.org/0000-0002-9211-4093
São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

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Published

2020-09-25

How to Cite

Ocampo, P. E., Vallejo, V., Montoya, L. M., Rocha, N. S., Landim, F. da C., & Rahal, S. C. (2020). Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells. Revista Colombiana De Ciencias Pecuarias, 34(3), 212–223. https://doi.org/10.17533/udea.rccp.v34n3a06

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