Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells


  • Pablo E. Ocampo São Paulo State University
  • Viviana Vallejo São Paulo State University
  • Luis M. Montoya National University of Colombia
  • Noeme S. Rocha São Paulo State University
  • Fernanda da C. Landim São Paulo State University
  • Sheila C. Rahal São Paulo State University




cell differentiation, cartilage, chondrocytes, chondrogenesis, chondrogenic differentiation, collagen, corticosteroids, glucocorticoid, histology, hyaluronic acid, mesenchymal stem cell, osteoarthritis, triancinolone acetonide, viscosupplementation


Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.

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Author Biographies

Pablo E. Ocampo, São Paulo State University

São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

Viviana Vallejo, São Paulo State University

São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

Luis M. Montoya, National University of Colombia

Faculty of Veterinary Medicine, National University of Colombia, Bogotá, Colombia. Research Group in Veterinary Pathology.

Noeme S. Rocha, São Paulo State University

São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

Fernanda da C. Landim, São Paulo State University

São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.

Sheila C. Rahal, São Paulo State University

São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu.


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How to Cite

Ocampo, P. E., Vallejo, V., Montoya, L. M., Rocha, N. S., Landim, F. da C., & Rahal, S. C. (2020). Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells. Revista Colombiana De Ciencias Pecuarias, 34(3), 212–223. https://doi.org/10.17533/udea.rccp.v34n3a06



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