Prevalence of Ehrlichia canis and Hepatozoon canis in sheltered dogs in southern Aburrá Valley, Colombia
DOI:
https://doi.org/10.17533/udea.rccp.v35n2a01Keywords:
Colombia, diagnosis, dog, Ehrlichia canis, Hepatozoon canis, infection, polymerase chain reaction, tick-borne pathogensAbstract
Background: Bacteria of the Anaplasmataceae family and canine hemoparasitic protozoans transmitted by ticks are common in Colombia due to circulation and biological adaptation of vector Rhipicephalus sanguineus sensu lato (s.l.). Objective: To detect the circulation of Ehrlichia canis and Hepatozoon canis in sheltered dogs in three municipalities in southern Aburrá Valley, province of Antioquia, Colombia. Methods: Primers were used to amplify the 16S rRNA associated with the Anaplasmataceae family, dsb for Ehrlichia sp. and 18S rRNA for Hepatozoon sp. Results: Of the 357 samples of venous blood obtained, representing all the sheltered dogs in the study zone, Ehrlichia canis DNA was detected in 2.2% of individuals, showing identity of 100% with previous sequences from the GenBank. Hepatozoon canis showed 8.7% (31/357) prevalence of infection, with 100% identity to genotypes from Japan, Brazil, and Spain. Only one sequence of H. canis exhibited a phylogenic divergence concerning H. canis previously reported in Brazil and the Old World. Conclusions: This study confirms the circulation of E. canis and H. canis in asymptomatic shelter dogs in the south-central zone of the Aburrá Valley, province of Antioquia, Colombia. The present study is the first molecular detection of H. canis in the Province of Antioquia and the third report of canine hepatozoonosis from Colombia, highlighting the importance of considering this agent in veterinary clinic.
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Almazán C, González-Álvarez VH, Fernández IG, Cabezas-Cruz A, Rodríguez-Martínez R., de la Fuente J. Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico. Ticks Tick Borne Dis 2016; 7(2):276–283. DOI: https://doi.org/10.1016/j.ttbdis.2015.11.002
Arroyave E, Cornwell ER, McBride JW, Díaz CA, Labruna MB, Rodas JD. Detection of tick-borne rickettsial pathogens in naturally infected dogs and dog-associated ticks in Medellin, Colombia. Rev Bras Parasitol Vet 2020a; 29(3):e005320. DOI: https://dx.doi.org/10.1590/s1984-29612020060
Arroyave E, Rodas-González JD, Zhang X, Labruna MB, González MS, Fernández-Silva JA, McBride JW. Ehrlichia canis TRP36 diversity in naturally infected dogs from an urban area of Colombia. Ticks Tick Borne Dis 2020b; 11(3):101367. DOI: https://doi.org/10.1016/j.ttbdis.2019.101367
Baneth G, Barta JR, Shkap V, Martin DS, Macintire DK, Vincent-Johnson N. Genetic and antigenic evidence supports the separation of Hepatozoon canis and Hepatozoon americanum at the species level. J Clin Microbiol Infect 2000; 38(3):1298–1301. DOI: https://doi.org/10.1128/JCM.38.3.1298-1301.2000
Barrantes-González AV, Jiménez-Rocha AE, Romero JJ, Dolza G. Serology, molecular detection and risk factors of Ehrlichia canis infection in dogs in Costa Rica. Ticks and tick-borne diseases 2016; 7(6): 1245-1251. DOI: https://doi.org/10.1016/j.ttbdis.2016.07.006
Bonilla LM, Cardona SB, Cardona DR, Jaramillo JE, Rivera DG, Echeverri ÉT, Echeverri CA. Implementación de un método basado en PCR, para el diagnóstico de Ehrlichia spp., en caninos de Medellín (Colombia). Rev CES Med Vet & Zootec 2012; 7(2):38–46. URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S1900-96072012000200005
Chinchilla D, Thomas R, Raquel-Castro L. PCR tiempo real para detección de Hepatozoon canis en perros de la ciudad de Cúcuta, Colombia. Rev Inv Vet Perú 2020; 31(3):e16429-e16429. DOI: http://dx.doi.org/10.15381/rivep.v31i3.16429
Criado-Fornelio A, Rey-Valeiron C, Buling A, Barba-Carretero JC, Jefferies R, Irwin P. New advances in molecular epizootiology of canine hematic protozoa from Venezuela, Thailand and Spain. Vet Parasitol 2007; 144 (3-4):261–269. DOI: https://doi.org/10.1016/j.vetpar.2006.09.042
Dantas-Torres F, Chomel BB, Otranto D. Ticks and tick-borne diseases: A One Health perspective. Trends Parasitol 2012; 28(10):437–446. DOI: https://doi.org/10.1016/j.pt.2012.07.003
Dantas-Torres F, Otranto D. Rhipicephalus sanguineus on dogs: relationships between attachment sites and tick developmental stages. Exp Appl Acarol 2011; 53: 389–397. DOI: https://doi.org/10.1007/s10493-010-9406-4
de la Fuente J, Torina A, Naranjo V, Nicosia S, Alongi A, La Mantia F, Kocan KM. Molecular characterization of Anaplasma platys strains from dogs in Sicily, Italy. BMC Vet Res 2006; 2(24):1-11. DOI: https://doi.org/10.1186/1746-6148-2-24
de Miranda RL, O’Dwyer LH, de Castro JR, Metzger B, Rubini AS, Mundim AV, Eyal O, Talmi-Frank D, Cury MC, Baneth G. Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil. Res Vet Sci 2014; 97(1):325–328. DOI: https://doi.org/10.1016/j.rvsc.2014.06.015
Demoner L, Antunes JM, O’Dwyer LH. Canine hepatozoonosis in Brazil: aspects of the biology and transmission. Rev Fac Med Vet Univ Sao Paulo 2013; 20(2):193-202.
Eiras DF, Basabe J, Scodellaro CF, Banach DB, Matos ML, Krimer A, Baneth G. First molecular characterization of canine hepatozoonosis in Argentina: evaluation of asymptomatic Hepatozoon canis infection in dogs from Buenos Aires. Vet Parasitol 2007; 149(3-4):275–279. DOI: https://doi.org/10.1016/j.vetpar.2007.07.010
Evans RB, O'Connor A. Statistics and evidence-based veterinary medicine: answers to 21 common statistical questions that arise from reading scientific manuscripts. Vet Clin North Am Small Anim Pract. 2007; 37(3):477-86. DOI: https://doi.org/10.1016/j.cvsm.2007.01.006
Forlano MD, Teixeira KR, Scofield A, Elisei C, Yotoko KS, Fernandes KR, Linhares GF, Ewing SA, Massard CL. Molecular characterization of Hepatozoon sp. from Brazilian dogs and its phylogenetic relationship with other Hepatozoon spp. Vet Parasitol 2007; 145(2):21–30. DOI: https://doi.org/10.1016/j.vetpar.2006.10.023
Jarquín-Díaz VH, Barbachano A, Maldonado R, Vásquez AA, Aguilar JL. First molecular evidence of Hepatozoon canis in domestic dogs and ticks in fragmented rainforest areas in Mexico. Vet Parasitol Reg Stud Reports 2017; 6(1):4–8. DOI: https://doi.org/10.1016/j.vprsr.2016.11.001
Jefferies R, Ryan U, Muhlnickel CP. Two Species of Canine Babesia in Australia: Detection and Characterization by PCR. Vet Parasitol 2003; 89:409–412. DOI: https://doi.org/10.1645/0022-3395(2003)089[0409:TSOCBI]2.0.CO;2
Little SE. Ehrlichiosis and anaplasmosis in dogs and cats. Vet Clin North Am Small Anim Pract 2010; 40(6):1121-1140. DOI: https://doi.org/10.1016/j.cvsm.2010.07.004
O’Dwyer LH. Brazilian canine hepatozoonosis. Rev Bras Parasitol Vet 2011; 20(3):181–193. DOI: https://doi.org/10.1590/S1984-29612011000300002
Peleg O, Baneth G, Eyal O, Inbar J, Harrus S. Multiplex real-time qPCR for the detection of Ehrlichia canis and Babesia canis vogeli. Vet Parasitol 2010; 173:292–299. DOI: https://doi.org/10.1016/j.vetpar.2010.06.039
Posada-Zapata J, Cabrera A, González–Alvarez D, Rodas JD, Monsalve S, Londoño A. Identificación de bacterias de la familia Anaplasmataceae en un albergue canino del municipio de Caldas, Antioquia. Rev MVZ Córdoba 2017; 22(1):6014. DOI: https://doi.org/10.21897/rmvz.1071
Rojas A, Rueda A, Díaz D, Mesa NC, Benavides J, Imbachi K, Álvarez L, López R. Identificación de Ehrlichia canis (Donatien & Lestoquard) Moshkovski mediante PCR anidada. Vet y Zootec 2013; 7(1):37–48. DOI: http://vetzootec.ucaldas.edu.co/downloads/v7n1a03.pdf
Shaw DK, Wang X, Brown LJ, Chávez AS, Reif KE, Smith A, Scott AJ, McClure EE, Boradia VM, Hammond HL, Sundberg EJ, Snyder G, Liu L, DePonte K, Villar M, Ueti MW, de la Fuente J, Ernst RK, Pal U, Fikrig E, Pedra JH. Infection-derived lipids elicit an immune deficiency circuit in arthropods. Nat Commun 2017; 8(12):14401. DOI: https://doi.org/10.1038/ncomms14401
Soares R, Ramos CA, Pedroso T, Babo-Terra V, Cleveland H, De Araújo F, Ramos C. Molecular survey of Anaplasma platys and Ehrlichia canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil. An Acad Bras Cienc 2017; 89(1):301–306. DOI: https://doi.org/10.1590/0001-3765201720150556
Spolidorio MG, Labruna MB, Zago AM, Donatele DM, Caliari KM, Yoshinari NH. Hepatozoon canis infecting dogs in the State of Espírito Santo, southeastern Brazil. Vet Parasitol 2009; 163:357–361. DOI: https://doi.org/10.1016/j.vetpar.2009.05.002
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30(12):2725-2729. DOI: https://doi.10.1093/molbev/mst197
Vargas-Hernández G, André MR, Muñoz TD, Faria JM, Machado RZ, Tinucci-Costa M. Molecular characterization of Hepatozoon canis in dogs from Colombia. Parasitol Res 2012; 110:489–492. DOI: https://doi.org/10.1007/s00436-011-2634-7
Viana M, Mancy R, Biek R, Cleaveland S, Cross PC, Lloyd-Smith JO, Haydon DT. Assembling evidence for identifying reservoirs of infection. Trends Ecol Evol 2014; 29(5):270-279. DOI: https://doi.org/10.1016/j.tree.2014.03.002
Vieira F, Acosta IC, Martins TF, Filho JM, Krawczak F, Barbieri AR, Egert L, Fernandes DR, Braga FR, Labruna MB, Dietze R. Tick-borne infections in dogs and horses in the state of Espírito Santo, Southeast Brazil. Vet Parasitol 2018; 249(1):43–48. DOI: https://doi.org/10.1016/j.vetpar.2017.11.005
Waner T, Nachum-Biala Y, Harrus S. Evaluation of a commercial in-clinic point-of-care polymerase chain reaction test for Ehrlichia canis DNA in artificially infected dogs. Vet Parasitol 2014; 202(3):618–621. DOI: https://doi.org/10.1016/j.tvjl.2014.10.004
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