Aplicación de la prueba de micronúcleos a especies ícticas silvestres frecuentes en dos ambientes lenticos de zonas bajas de Colombia

Autores/as

  • Isabel Palacio-Betancur Universidad de Antioquia
  • Jaime A. Palacio-Baena Universidad de Antioquia
  • Mauricio Camargo-Guerrero Universidad de Antioquia

DOI:

https://doi.org/10.17533/udea.acbi.4730

Palabras clave:

micronúcleos, especies ícticas, genotoxicidad, ambientes lénticos, Colombia

Resumen

se evaluó la presencia de estrés genotóxico en algunas especies ícticas en dos ambientes lénticos tropicales de Colombia [Cienaga de Cachimbero (Santander) y Cienaga de Ayapel (Cordoba)] a través de la aplicación de la prueba de micronúcleos. Después de la captura de los peces y la identificación de las especies se extrajo la muestra de sangre por medio de punción cardiaca y se tomaron las medidas morfométricas. las muestras se tiñeron con Giemsa y se cuantificó la frecuencia de micronúcleos en 2.000 células por individuo. Se calculó el factor de con-dición para cada uno de los ejemplares capturados y se aplicó un análisis de K-W para comparar el número de MN entre las diferentes especies en cada una de las ciénagas y el número de mn de las especies comunes en los dos sistemas lagunares. Los resultados mostraron una relación estadísticamente significativa entre el factor de condición y la frecuencia de eritrocitos micronúcleados. Aunque no existen diferencias estadísticamente significativas entre las frecuencias de los micronúcleos de las diferentes especies en las ciénagas de cachimbero y de Ayapel, Hoplias malabaricus presentó un promedio de micronucleos muy superior a las demás especies en la ciénaga de Cachim-bero y Ctenolucius sujeta y Cyphocharax magdalenae para la ciénaga de Ayapel. nuestros resultados sugieren que tanto especies sensitivas como no sensitivas podrían ser incluidas como especies centinelas en los estudios de biomonitoreo en colombia.

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Ali d, nagpure ns, Kumar s, Kumar r, Kushwaha B. 2008.Genotoxicity assessment of acute exposure of chlo-rpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Chemosphere, 71: 1823-1831.

Al-sabti K. 1986. comparative micronucleated erythrocyte cell induction in three cyprinids by five carcinogenic-mutagenic chemicals. cytobios, 47 (190-191): 147-154.

Al-sabti K. 1994. micronuclei induced by selenium, mer-cury, methylmercury and their mixtures in binucleated blocked fish erythrocyte cells. Mutation Research, 320 (1-2): 157-163.

Al-Sabti K. 1995. An in vitro binucleated blocked hepatic cell technique for genotoxicity testing in fish. Mutation research, 335 (2): 109-120.

Al-sabti K, Franko M, Andrijanic B, Stegnar S. 1994. Chro-mium induced micronuclei in fish. Journal of Applied toxicology, 14: 33-36.

Al-Sabti k, Metcalfe CD. 1995. Fish micronuclei for as-sessing genotoxicity in water. mutation research,343 (2-3): 121-135.

Arcand-Hoy, metcalfe cd. 2000. Hepatic micronuclei in brown bulheads (Ameiurus nebulosus) as a biomarker for exposure to genotoxic chemicals. journal of Great Lakes Research, 26 (4): 408-415.

Arkhipchuk VV, Garanko NN. 2005. Using the nucleolar bio-marker and the micronuclei test on in vivo fish fin cells. ecotoxicology and environmental safety, 62: 42-52.

Bahari iB, noor f, daud nm. 1994. micronucelated eryth-rocytes as an assay to assess actions by physical and chemical genotoxic agents in Clarias gariepinus. muta-tion research, 313: 1-5.

Belpaeme K, Delbeke K, Zhu L, Kirsch-VM. 1996.cytoge-netic studies of Pc B77 on brown trout (Salmo truttafario) using the micronuclei test and the alkaline comet assay. mutagenesis, 11: 485-495.

Bombail v, Aw d, Gordon e, Batty j. 2001. Application of the comet and micronuclei assays to butterfish (Pholis gunnellus) erythrocytes from the firth of forth, scot-land. chemosphere, 44: 383-392.

Campana MA, Panzeri AM, Moreno VJ, Dulout FN. 1999. Genotoxic evaluation of the pyrethroid lambda-cyhalothrin using the micronuclei test in erythrocytes of the fish Cheirodon interuptus interuptus. mutation research, 438: 155-161.

Carrasco K, tibury l, myers m. 1990. Assessment of the piscine micronuclei test as an in situ biological indicator of chemical effects. canadian journal of fisheries and Aquatic science, 27 (11): 2123-2136.

cavas t. 2008. in vivo genotoxicity of mercury chloride and lead acetate: micronucleus test on acridine orange stained fish cells. Food and Chemical Toxicology, 46: 352-358.

Cavas T, Ergene-Gözükara S.2005. Induction of micronuclei and nuclear abnormalities in Oreochomis niloticus fol-lowing exposure to petroleum refinery and chromium processing plant effluents. Aquatic Toxicology,74 (3): 264-271.

cavas t, Könen s. 2008. in vivo genotoxicity testing of the amnesic shellfish poison (domoic acid) in piscine erythrocytes using the micronucleus test and the comet assay. Aquatic toxicology, 90: 154-159.

das rK, nanda nK. 1986. induction of micronuclei in pe-ripheral erythrocytes of fish Heteropneustes fossilis by mitomycin C and paper mill effluent. Mutation Research letters, 175 (2): 67-71.

d’Agostini f, di marco c, melodia f, vigano l. 1992. cito-genetic damage in fish (Oncorhynchus mykiss) exposed in situ to water from a polluted river. Bollettino-societa italiana Biologia sperimentale, 68: 549-553.

de flora s, vigano l, d’Agostini f, camoriano A, Bagnasco m, Bennicelli c, melodia f, Arillo A. 1993. multiple genotoxicity biomarkers in fish exposed in situ to pol-luted river water. mutation research, 319: 167-177.

fenech m, morley AA. 1985. measurement of micronuclei in lymphocytes. mutation reseach, 285: 35-44.

Grisolia Kc and Koppe c. 2002. A comparison between mouse and fish micronuclei test using cyclophoph-amide, mitomicina c and various pesticides. mutation research, 518: 145-150.

Grisolia cK, starling flrm. 2001. micronuclei monitoring of fishes from lake Paranoá, under influence of sewage treatment plant discharges. mutation research, 491: 39-44.

Hayashi mt, ueda t, uyeno K, Wada K, Kinae n, saotome K, Tanaka N, Takai A, Sasaki YF, Asano N, Sofuni T, Ogima Y. 1998. Development of genotoxicity assay systems that use aquatic organisms. mutation research,399 (2): 217-225.

Henao B, Palacio jA, camargo m. 2005. evaluación genotóxica de los plaguicidas cipermetrina y diacinón n tilapia roja (Oreochromis sp.). Actualidades Biológicas, 27 (82): 43-55.

Hooftman rn, raat WK. 1982. induction of nuclear anoma-lies (micronuclei) in the peripheral blood erythrocytes of eastern mudminnow Umbra pygmaea by ethylmethane sulphonate. mutation research, 104: 147-152.

Hose je, cross jn, smith sG, diehl d. 1987. elevated circulating erythrocyte micronuclei in fishes from contaminated sites of southern california. marine en-vironmental research, 22 (3): 167-176.

Hoshina mm, Angelis df, marin-morales mA. 2008. induction of micronucleus and nuclear alterations in fish (Oreochromis niloticus) by a petroleum refinery effluent. Mutation Research/ Genetic Toxicology and environmental mutagenesis, 656 (1-2): 19-26

Hughes jB, Hebert At. 1991. erythrocyte micronuclei in winter flounder (Pseudopleuronectes americanus) result of field survey during 1980-1988 from Virginia to Nova scotia and in long island sound. Archives of environ-mental contamination and toxicology, 20: 167-177.

Ilyinskikh N, Ilyinskikh EN, Ilyinskikh IN. 1998. Micronu-cleated erythocytes frecuency and radiosium bioconcen-tration in pikes (Esox lucius) caught in the tom river near the nuclear facilities of the siberian chemical com-plex (Tomsk-7). Mutation Research, 421 (2): 197-203.

Kirsch-Volders M, Elhajouji A, Cundari E, Van Hummelem P. 1997. the in vitro micronuclei test: a multi-endpoint assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. Mutation Research/Genetic Toxicology and environmental mutagenesis, 392 (1-2): 19-30.

leprieur f, Beauchard o, Blanchet s, oberdorff t, Brosse s. 2008. fish invasions in the world’s river systems: When natural processes are blurred by human activities. PLoS Biology 6(2): e28.

Manna GK, Banerjee G, Gupta S. 1985. Micronuclei test in the peripheral erythrocytes of the exotic fish, Oreo-chromis massambica. nucleus, 28: 176-179.

Majone F, Brunetti R, Gola I, Levis A. 1987. Persistence of micronuclei in the marine mussel, Mytilus gallopro-vincialis, after treatment with mitomycin c. mutation research, 191:157-161.

marlasca mj, sampera c. 1998. Hepatic alterations and in-duction of micronuclei in rainbow trout (Oncorhynchus mykiss) exposed to a textile industry effluent. Histology and Histopathology, 13 (3): 703-712.

metcalfe cd. 1988. induction of micronuclei and nuclear ab-normalities in the erythrocytes of mudminnow (Umbra limi) and brown bulheads (Ictalurus nebulosus). Bull. environ. contam. toxicology, 40: 489-495.

Minissi S, Ciccotti E, Rizzoni M.1996. Micronuclei test in erythrocytes of Barbus plebejus ( teleostei, Pisces) from two natural environments: a bioassay for the in situ de-tection of mutagens in freshwater. mutation research, 367 (4): 245-251.

nepomuceno j, ferrari i, spaño m, centeno A. 1997. detec-tion of micronuclei in peripheral erythrocytes of Cypri-nus carpio exposed to metallic mercury. environmental and molecular mutagenesis, 30 (3): 293-297.

Odelgah PGC, Osanyipeju AO.1995. Genotoxic effects of two industrial effluents and ethyl methane sulfonate in Clarias lasera [published erratum appears in food Chemical Toxicology, 1996, Feb: 34 (2)]. Food and chemical toxicology, 33 (6): 501-505.

Plan de Manejo Ambiental del Complejo de Humedales de Ayapel. Informe final del proyecto CVS. facultad de ingeniería. medellín (Antioquia), colombia: universidad de Antioquia.

Pastor S. 2002. Biomonitorización citogenética de cuatro poblaciones agrícolas europeas expuestas a plaguicidas, mediante el ensayo de micronúcleos [tesis de doctora-do]. [España]: Universidad Autónoma de Barcelona.

Peñalosa m, camargo m, Palacio j. 2003. Genotoxicidad del cloruro de mercurio en dos especies ícticas (Prochilodus magdalenae y Oreochromis sp.) Actualidades Biológi-cas, 25 (79): 105-111.

Poongothai K, shayin s, usharani mv. 1996. induction of micronuclei in fish by polluted water and heavy metals. cytobios, 86 (344): 17-22.

Porto JIR, Araujo CSO, Feldberg E. 2005. Mutagenic effects of mercury pollution as revealed by micronuclei test on three Amazonian fish species. Environmental Research, 97: 287-292.

Reyes-Gavilan F, Garrido R, Nicieza AG, Toledo MM, Braña f. 1996. fish community variation along physical gradi-ents in short streams of northern spain and the disruptive effect of dams. Hydrobiology, 321: 155-163.

Ricker WE. 1975. Computation and interpretation of bio-logical statistics of fish population. Bulletin Fisheries research Board of canada, 191: 1-138.

Rodriguez-Cea A, Ayllon F, Garcia-VE. 2003. Micronuclei test in freshwater fish species: an evaluation of its sen-sitivity for application in field surveys. Ecotoxicology and environmental safety, 56: 442-448.

Sanchez-Galan S, Linde AR, Izquierdo JI, Garcia-VE. 1998. Micronuclei and fluctuating asymmetry in brown trout (Salmo trutta): complementary methods of biomonitor freshwater ecosystems. mutation research, 412 (3): 219-225.

Sanchez-Galan S, Linde AR, Izquierdo JI, Garcia-VE. 1999. Brown trout and european minnow as target species for genotoxicity test: differential sensitivity to heavy metals. ecotoxicology and environment safety, 43: 301-304.

Wrisberg mn, van der Gaag mA. 1992. in vivo detection of genotoxicity in waste water from a wheat and straw paper pulp factory. science of the total environment, 121: 95-108.

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Publicado

2010-02-03

Cómo citar

Palacio-Betancur, I., Palacio-Baena, J. A., & Camargo-Guerrero, M. (2010). Aplicación de la prueba de micronúcleos a especies ícticas silvestres frecuentes en dos ambientes lenticos de zonas bajas de Colombia. Actualidades Biológicas, 31(90), 1–11. https://doi.org/10.17533/udea.acbi.4730

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