Bovine kappa-casein and its association with somatic cell count in holstein cattle

Authors

  • José Usme-Ciro Universidad de Antioquia
  • Fernando Restrepo Universidad de Antioquia, Facultad de Ciencias Agrarias
  • Esperanza Trujillo-Bravo Universidad de Antioquia, Instituto de Biología

DOI:

https://doi.org/10.17533/udea.acbi.329454

Keywords:

CSN3, factorial analysis, allelic frequency, genotypic frequency, antimicrobial activity, milk

Abstract

It was evaluated the association of genotypes AA, AB and BB of kappa-casein (CSN3) and the somatic cells count (SCC),as well as the relation between the occurrence of recurrent clinical mastitis and the genotypes, using a simple correlation.A total of 145 Holstein breed cows were sampled for milk and blood in different farms of department of Antioquia,Colombia. A fragment of the CSN3 gene was amplified by polymerase chain reaction; then, the amplified fragment was restricted with endonuclease Hinf I, and visualized on agarose gel electrophoresis. For the milk samples, the SCC was classified in two ranges: the first included animals which SCC did not exceed 300 x 103 cells/ml, and the second includes animals with a SCC higher than 300 x 103 cells/ml. It was detected a weak association between the genotype AA and lowSCC, and the genotype AB and the high SCC. This association was higher for the genotype AA than for AB.

|Abstract
= 354 veces | PDF (ESPAÑOL (ESPAÑA))
= 207 veces|

Downloads

Download data is not yet available.

Author Biography

José Usme-Ciro, Universidad de Antioquia

Laboratorio de Genética y Mejoramiento Animal, Instituto de Biología, Universidad de Antioquia, A. A. 1226, Medellín.

References

Ali AKA, Shook GE. 1980. An optimum transformation for so-matic cell concentration in milk. J Dairy Sci 63:487.Bawden WS, Nicholas KR. 1999. Molecular genetics of milkproduction. En: Fries R, Ruvinsky A (eds.). The Genetics ofCattle. Wallingford, CAB International, pp. 539-576.Bellamy WR, Yamauchi K, Wakabayashi H, Takase M, Shima-mura S, Tomita M. 1994. Antifungal properties of lactofe-rricin, a peptide derived from the N-terminal region ofbovine lactoferrin. Lett Appl Microbiol 18:230-233.CISIA –Centre International de Statistique et d’Informatique Appli-quées–. 1995. SPADN version 3.0. Saint-Mandé, France.Clare DA, Swaisgood HE. 2000. Bioactive milk peptides: A pros-pectus. J Dairy Sci 83:1187-1195.Craven N, Williams MR. 1985. Defences of the bovine mamma-ry gland against infection and prospects for their enhance-ment. Vet Immunol Immunopathol 10:71-127.Dabdoub SAM. 1984. Genetics and phenotypic relationshipamong mastitis, Somatic Cell concentration and milk yield.Ph.D. Dissertation. University of Wisconsin, Madison.Dovc P. 2000. Genetic polymorphisms in milk protein genes andtheir impact on milk composition. Adv Exp Med Biol480:225-230.Eberhart RJ, Hutchinson LJ, Spencer SB. 1982. Relationshipsof bulk tank somatic cell counts to prevalence of intrama-mmary infection and to indices of herd production. J FoodProtect 45:1125-1128.Guo SW, Thompson EA. 1992. Performing the exact test of Har-dy-Weinberg proportion of multiple alleles. Biometrics48:361-372

Harmon RJ. 1994. Physiology of mastitis and factors affectingsomatic cell counts. J Dairy Sci 77:2103-2112.Harmon RJ, Heald CW. 1982. Migration of polymorphonuclearleucocytes into the bovine mammary gland during experi-mentally induced Staphylococcus aureus mastitis. Am J VetRes 43:992-998.Harmon RJ, Langlois BE. 1986. Prevalence of minor pathogensand associated somatic cell counts. Proc 25th Annu Mtg NatlMastitis Counc Columbus, OH. Natl Mastitis Counc Inc,Arlington, VA, p. 11.Heringstad B, Klemetsdal G, Ruane J. 1999. Clinical mastitis inNorwegian cattle: frequency, variance components, and ge-netic correlation with protein yield. J Dairy Sci 82:1325-1330.Heyen DW, Weller JI, Ron M, Band M, Feldmesser E, Da Y,Wiggans GR, Vanraden PM, Lewin HA. 1998. Genomescan for QTL influencing milk production and health traitsin dairy cattle. Proc XXVIth Int Conf Anim Genet, 9-14August, 1998, Auckland, New Zealand, p. E006.Heyen DW, Weller JI, Ron M, Band M, Beever JE, FeldmesserE, Da Y, Wiggans GR, Vanraden PM, Lewin HA. 1999. Agenome scan for QTL influencing milk production andhealth traits in dairy cattle. Physiol Genomics 1:165-175.Jenness R. 1974. Caseins and caseinate micelles of various spe-cies. Neth Milk Dairy J 27:251-257.Kalm E, Reinsch N, Xu H, Thomsen H, Looft C, Grupe S,Brockmann G, Kühnn C, Schwerin M, Leyle B, Hiendle-der S, Erhardt G, Medjgorac I, Russ I, Förster M, BrenigB, Reinhard F, Reets R, Averdunk G. 1998. Mapping QTLon cattle chromosome 2, 5, 10, 16, 18 and 23. Anim Genet21(supl. 1):62

Published

2017-11-22

How to Cite

Usme-Ciro, J., Restrepo, F., & Trujillo-Bravo, E. (2017). Bovine kappa-casein and its association with somatic cell count in holstein cattle. Actualidades Biológicas, 26(80), 1–6. https://doi.org/10.17533/udea.acbi.329454

Issue

Section

Full articles