Enzymatic production of ethyl esters from castor oil

Claudia Delgado; Vaselina Pashova

doi:10.17533/udea.redin.14004

Authors

Claudia Delgado National University of Colombia
Vaselina Pashova National University of Colombia

DOI:

https://doi.org/10.17533/udea.redin.14004

Keywords:

castor oil, biodiesel, transesterification, lipase, Ping-Pong Bi Bi mechanism

Abstract

iodiesel synthesis through transesterificación of castor oil with ethanol using n-hexane as a solvent and a lipozyme TL IM commercial lipase as a biocatalyst was studied. The effect of ethanol molar relation to oil (3:1-10:1), temperature of reaction (35-65ºC), initial enzymatic charge (5-20% p/p) and amount of water (0-10% p/p) was investigated. The maximum percentage of bio-diesel (ethyl-esters) was obtained at a temperature of 35ºC, a 5:1 ethanol to oil molar ratio, an initial enzymatic charge of 15 % p/p and an initial water concentration of 2% p/p, both of them based on the castor oil weight. A simplified model was proposed to describe the enzymatic kinetics of castor oil transesterification with ethanol, based on the ping-pong Bi Bi mechanism with competitive inhibition for alcohol. The kinetic parameters of the model were estimated graphically and parametrically; a good approach among the results foreseen by the model and experimental data was found at low ethanol concentration (40-80 mol Kg-1).

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