Low frequency of enterococcus faecalis in the oral mucosa of subjects attending dental consultation

  • Carolina Carrero-Martínez Pontificia Universidad Javeriana
  • María Cristina González-Gilbert Pontificia Universidad Javeriana
  • María Alexandra Martínez-Lapiolo Pontificia Universidad Javeriana
  • Fátima Serna-Varona Pontificia Universidad Javeriana
  • Hugo Díez-Ortega Pontificia Universidad Javeriana
  • Adriana Rodríguez-Ciodaro Pontificia Universidad Javeriana
Keywords: Enterococcus faecalis, Oral mucosa, Oral microflora, Endondontics, Apical periodontitis

Abstract

Introduction: Enterococcus faecalis (E. faecalis) is a Gram-positive, facultative anaerobic, unsporulated coccus whose habitat is the gastrointestinal tract. It can also be found in the hepatobiliary tract, the vagina, and wounds on soft tissues. It has been shown that due to its high virulence, E. faecalis can penetrate the dentinal tubules, surviving chemical-mechanical instrumentation, colonize them at a depth of 300 µm and re-infest the canals even after being obturated, but the source of infection is not clear. The objective of this study was to determine the frequency and resistance profile of E. faecalis in the oral mucosa of patients attending dental consultation. Methods: samples were collected from the gingiva, gingival sulcus, palate, tongue, and cheeks of 200 adult subjects attending dental consultation. E. faecalis was identified by means of a screening that included catalase, hemolysis in agar blood, bile esculin, 6.5% NaCl, and PYR, confirming by a Microscan panel (DadeBehring). Results: E. faecalis was isolated from the oral microbiota of 10 samples (5%). Conclusions: this study found low frequencies of E. faecalis in the oral mucosa of subjects attending dental consultation.

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Author Biographies

Carolina Carrero-Martínez, Pontificia Universidad Javeriana

MD, Endodontics Specialist, Pontificia Universidad Javeriana. Private endodontics practice. Bogotá, Colombia.

María Cristina González-Gilbert, Pontificia Universidad Javeriana

MD, Universidad de Carabobo, Venezuela. Endodontics Specialist, Pontificia Universidad Javeriana. Private endodontics practice, Valencia, Venezuela 

María Alexandra Martínez-Lapiolo, Pontificia Universidad Javeriana

MD, Universidad de Carabobo, Venezuela. Endodontics Specialist, Pontificia Universidad Javeriana. Private endodontics practice, Barquisimeto, Venezuela.

Fátima Serna-Varona, Pontificia Universidad Javeriana

MD, Pontificia Universidad Javeriana. Pharmacology Specialist, Universidad Nacional de Colombia, MSc in Education, Pontificia Universidad Javeriana. Assistant Professor, School of Dentistry, Pontificia Universidad Javeriana, Colombia.

Hugo Díez-Ortega, Pontificia Universidad Javeriana

Bacteriologist, MSc in Microbiology, PhD in Biological Sciences, Pontificia Universidad Javeriana. Professor, School of Sciences, Pontificia Universidad Javeriana, Colombia

Adriana Rodríguez-Ciodaro, Pontificia Universidad Javeriana

Bacteriologist, MSc in Microbiology, Pontificia Universidad Javeriana Bogotá. Associate Professor, Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Colombia.

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Published
2015-04-20
How to Cite
Carrero-Martínez C., González-Gilbert M. C., Martínez-Lapiolo M. A., Serna-Varona F., Díez-Ortega H., & Rodríguez-Ciodaro A. (2015). Low frequency of enterococcus faecalis in the oral mucosa of subjects attending dental consultation. Revista Facultad De Odontología Universidad De Antioquia, 26(2), 261-270. Retrieved from https://revistas.udea.edu.co/index.php/odont/article/view/15010