Low frequency of enterococcus faecalis in the oral mucosa of subjects attending dental consultation

Authors

  • Carolina Carrero-Martínez Pontificia Universidad Javeriana
  • María Cristina González-Gilbert Pontificia Universidad Javeriana
  • María Alexandra Martínez-Lapiolo Pontificia Universidad Javeriana
  • Fátima Serna-Varona Pontificia Universidad Javeriana
  • Hugo Díez-Ortega Pontificia Universidad Javeriana
  • Adriana Rodríguez-Ciodaro Pontificia Universidad Javeriana

DOI:

https://doi.org/10.17533/udea.rfo.15010

Keywords:

Enterococcus faecalis, Oral mucosa, Oral microflora, Endondontics, Apical periodontitis

Abstract

Introduction: Enterococcus faecalis (E. faecalis) is a Gram-positive, facultative anaerobic, unsporulated coccus whose habitat is the gastrointestinal tract. It can also be found in the hepatobiliary tract, the vagina, and wounds on soft tissues. It has been shown that due to its high virulence, E. faecalis can penetrate the dentinal tubules, surviving chemical-mechanical instrumentation, colonize them at a depth of 300 µm and re-infest the canals even after being obturated, but the source of infection is not clear. The objective of this study was to determine the frequency and resistance profile of E. faecalis in the oral mucosa of patients attending dental consultation. Methods: samples were collected from the gingiva, gingival sulcus, palate, tongue, and cheeks of 200 adult subjects attending dental consultation. E. faecalis was identified by means of a screening that included catalase, hemolysis in agar blood, bile esculin, 6.5% NaCl, and PYR, confirming by a Microscan panel (DadeBehring). Results: E. faecalis was isolated from the oral microbiota of 10 samples (5%). Conclusions: this study found low frequencies of E. faecalis in the oral mucosa of subjects attending dental consultation.

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Author Biographies

Carolina Carrero-Martínez, Pontificia Universidad Javeriana

MD, Endodontics Specialist, Pontificia Universidad Javeriana. Private endodontics practice. Bogotá, Colombia.

María Cristina González-Gilbert, Pontificia Universidad Javeriana

MD, Universidad de Carabobo, Venezuela. Endodontics Specialist, Pontificia Universidad Javeriana. Private endodontics practice, Valencia, Venezuela 

María Alexandra Martínez-Lapiolo, Pontificia Universidad Javeriana

MD, Universidad de Carabobo, Venezuela. Endodontics Specialist, Pontificia Universidad Javeriana. Private endodontics practice, Barquisimeto, Venezuela.

Fátima Serna-Varona, Pontificia Universidad Javeriana

MD, Pontificia Universidad Javeriana. Pharmacology Specialist, Universidad Nacional de Colombia, MSc in Education, Pontificia Universidad Javeriana. Assistant Professor, School of Dentistry, Pontificia Universidad Javeriana, Colombia.

Hugo Díez-Ortega, Pontificia Universidad Javeriana

Bacteriologist, MSc in Microbiology, PhD in Biological Sciences, Pontificia Universidad Javeriana. Professor, School of Sciences, Pontificia Universidad Javeriana, Colombia

Adriana Rodríguez-Ciodaro, Pontificia Universidad Javeriana

Bacteriologist, MSc in Microbiology, Pontificia Universidad Javeriana Bogotá. Associate Professor, Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Colombia.

References

Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 2005; 43(11): 5721-5732.

Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retratment. J Endod 2006; 32(2): 93-98.

He XS, Shi WY. Oral microbiology: past, present and future. Int J Oral Science 2009; 1(2): 47-58.

Love RM, Jenkinson HF. Invasion of dentinal tubules by oral bacteria. Crit Rev Oral Biol Med 2002; 13(2): 171-183.

Wang QQ, Zhang CF, Chu CH, Zhu XF. Prevalence of Enterococcus faecalis in saliva and filled root canals of teeth associated with apical periodontitis. Int J Oral Sci 2012; 4(1): 19-23.

D’Ercole S, Filippakos A, De Toledo Leonardo R, Pameijer CH, Tripodi D. Enterococcus faecalis leakage of root canal sealers: an ex vivo study. J Biol Regul Homeost Agents 2012; 26(3): 545-552.

Rôças IN, Siqueira JF Jr. Characterization of microbiota of root canal-treated teeth with posttreatment disease. J Clin Microbiol 2012; 50(5): 1721-1724.

Dahlen G, Blomqvist S, Almstahl A, Carlén A. Virulence factors and antibiotic susceptibility in enterococci isolated from oral mucosal and deep infections. J Oral Microbiol 2012; 4: doi 10.3402/jom.v4io.10855.

Zehnder M, Guggenheim B. The mysterious appearance of enterococci in filled root canals. Int Endod J 2009; 42(4): 277-287.

Pardi G, Guilarte C, Cardozo EI, Briceño EN. Detección de Enterococcus faecalis en dientes con fracaso en el tratamiento endodóntico. Acta Odontol Venez 2009; 47(1): 1-11.

Siquera JF, Rôças IN, Ricucci D. Biofilms in endodontic infection. Endodontic Topics 2010; 22(1): 33-49.

Zoletti GO, Pereira EM, Schuenck RP, Teixeira LM, Siqueira JF Jr, dos Santos KR. Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canals. Res Microbiol 2011; 162(2): 151-158.

Fisher K, Phillips C. The ecology, epidemiology and virulence of Enterococcus. Microbiology 2009; 155(6): 1749-1757.

Reffuveille F, Leneveu C, Chevalier S, Auffray Y, Rincé A. Lipoproteins of Enterococcus faecalis: bioinformatic identification, expression analysis and relation to virulence. Microbiology 2011; 157: 3001-3013.

Guerreiro-Tanomaru JM, de Faria-Júnior NB, Duarte MA, Ordinola-Zapata R, Graeff MS, Tanomaru-Filho M. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. J Endod 2013; 39(3): 346-350.

Centikaya Y, Falk P, Mayhall CG. Vancomycin-resistant enterococci. Clin Microbiol Rev 2000; 13(4): 686-707.

Kühn I, Iversen A, Burman LG, Olsson-Liljequist B, Franklin A, Finn M et al. Comparison of enterococcal populations in animals, humans, and the environment —a European study. Int J Food Microbiol 2003; 88(2-3): 133-145.

Kurrle E, Bhaduri S, Krieger D, Gaus W, Heimpel H, Pflieger H et al. Risk factors for infections of the oropharynx and the respiratory tract in patients with acute leukemia. J Infect Dis 1981; 144(2): 128-136.

Smyth CJ, Halpenny MK, Ballagh SJ. Carriage rates of enterococci in the dental plaque of hemodialysis patients in Dublin. Br J Oral Maxillofac Surg 1987; 25(1): 21-33.

Sedgley CM, Nagel AC, Shelburne CE, Clewell DB, Appelbe O, Molander A. Quantitative real-time PCR detection of oral Enterococcus faecalis in humans. Arch Oral Biol 2005; 50(6): 575-583.

Siqueira JR Jr. Aetiology of root canal treatment failure: why well-treated teeth can fail. Int Endod J 2001; 34(1): 1-10.

Duggan JM, Sedgley CM. Biofilm formation of oral and endodontic Enterococcus faecalis. J Endod 2007; 33(7): 815-818.

Zhu X, Wang Q, Zhang C, Cheung GS, Shen Y. Prevalence, phenotype, and genotype of Enterococcus faecalis isolated from saliva and root canals in patients with persistent apical periodontitis. J Endod 2010; 36(12): 1950-1955.

Chenoweth C, Schaberg D. The epidemiology of enterococci. Eur J Clin Microbiol Infect Dis 1990; 9(2): 80-89.

Kaklamanos EG, Charalampidou M, Menexes G, Topitsoglou V, Kalfas S. Transient oral microflora in Greeks attending day centres for the elderly and residents in homes for the elderly. Gerodontology 2005; 22(3): 158-167.

Bal BT, Yavuzyilmaz H, Yüzel M. A pilot study to evaluate the adhesion of oral microorganism to temporary soft lining materials. J Oral Sci 2008; 50(1): 1-8.

Kwang S, Abbott PV. Bacterial contamination of the fitting surfaces of restorations in teeth with pulp and periapical disease: a scanning electron microscopy study. Aust Dent J 2012; 57: 421-428.

Razavi A, Gmür R, Imfeld T, Zehnder M. Recovery of Enterococcus faecalis from cheese in the oral cavity of healthy subjects. Oral Microbiol Immunol 2007; 22(4): 248-251.

Luna NA, Santacruz AX, Palacios BD, Mafla AC. Prevalencia de periodontitis apical crónica en dientes tratados endodónticamente en la comunidad académica de la Universidad Cooperativa de Colombia, Pasto, 2008. Rev Fac Odontol Univ Antioq 2009; 21(1): 42-49.

Tobón S, Mesa A. Arismendi J, Domínguez J, Virgen AL. Nuevos enfoques en cirugía perirradicular. Revisión de literatura. Rev Fac Odontol Univ Antioq 2000; 11(2): 37-46.

Published

2015-04-20

How to Cite

Carrero-Martínez, C., González-Gilbert, M. C., Martínez-Lapiolo, M. A., Serna-Varona, F., Díez-Ortega, H., & Rodríguez-Ciodaro, A. (2015). Low frequency of enterococcus faecalis in the oral mucosa of subjects attending dental consultation. Revista Facultad De Odontología Universidad De Antioquia, 26(2), 261–270. https://doi.org/10.17533/udea.rfo.15010