Analysis of Enterococcus Faecalis, Staphylococcus Aureus, and Candida Albicans in cast metal cores

Authors

  • María del Pilar Angarita-Díaz Universidad Cooperativa de Colombia
  • Diana Forero-Escobar Universidad Cooperativa de Colombia
  • Nerly Fernanda Gutiérrez-Medina Universidad Cooperativa de Colombia
  • Francy Tatiana Yañez-Velandia Universidad Cooperativa de Colombia
  • Carlos Andrés Romero-Rojas Universidad Cooperativa de Colombia

DOI:

https://doi.org/10.17533/udea.rfo.v28n2a4

Keywords:

Cast core, Microbiological analysis, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.

Abstract


Introduction: all dental treatments should strictly follow aseptic protocols in order to reduce failure, especially when performing endodontic procedures. Despite being a key recommendation in this type of interventions, this statement is generally ignored, as students and clinicians tend to neglect the sterilization of posts prior to their use. To raise awareness on this practice, the objective of this study was to demonstrate the presence of microorganisms that cause failure, such as Enterococcus faecalis, Staphylococcus aureus and Candida albicans, in non-sterile cores. Methods: during the first half of 2016, fabricated cast cores were collected in the dental clinics of Universidad Cooperativa de Colombia at Villavicencio. The cores were immersed in saline solution making dilutions to up to 10–4, and finally inserted in duplicate into differential mediums for the microorganisms under study. The candidate colonies were then quantified and selected for the microorganisms under study, performing identification and confirmation in a certified clinical laboratory. Results: the presence of E. faecalis was detected in one of the cores (3.2%) used in the clinic, quantified in 5x104 CFU/ml. The presence of S. aureus or C. albicans was not identified, but other microorganisms were found, such as Candida parapsilopsis (35.5%), Candida tropicalis (6.5%), Kokuria kristinae (16.1%), Staphylococcus saprophyticus (12.9%) and Stenotrophomona maltophilia (3.2%). Conclusion: out of the microorganisms analyzed in this study, only E. faecalis was identified. However, other microorganisms associated with endodontic failure or other type of complications were identified.

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Author Biographies

María del Pilar Angarita-Díaz, Universidad Cooperativa de Colombia

PhD in Biotechnology. Professor of Oral Microbiology, researcher at the GIOMET. School of Dentistry, Universidad Cooperativa de Colombia, Villavicencio. 

Diana Forero-Escobar, Universidad Cooperativa de Colombia

DMD, Magister in Management. Program Head, School of Dentistry. Universidad Cooperativa de Colombia, Villavicencio 

Nerly Fernanda Gutiérrez-Medina, Universidad Cooperativa de Colombia

Ninth semester dental students. School of Dentistry. Universidad Cooperativa de Colombia, Villavicencio. This article is one of the requirements to qualify for the title of DMD 

Francy Tatiana Yañez-Velandia, Universidad Cooperativa de Colombia

Ninth semester dental students. School of Dentistry. Universidad Cooperativa de Colombia, Villavicencio. This article is one of the requirements to qualify for the title of DMD

Carlos Andrés Romero-Rojas, Universidad Cooperativa de Colombia

Ninth semester dental students. School of Dentistry. Universidad Cooperativa de Colombia, Villavicencio. This article is one of the requirements to qualify for the title of DMD

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Published

2017-06-30

How to Cite

Angarita-Díaz, M. del P., Forero-Escobar, D., Gutiérrez-Medina, N. F., Yañez-Velandia, F. T., & Romero-Rojas, C. A. (2017). Analysis of Enterococcus Faecalis, Staphylococcus Aureus, and Candida Albicans in cast metal cores. Revista Facultad De Odontología Universidad De Antioquia, 28(2), 292–310. https://doi.org/10.17533/udea.rfo.v28n2a4