Analysis of Enterococcus Faecalis, Staphylococcus Aureus, and Candida Albicans in cast metal cores


  • María del Pilar Angarita-Díaz Universidad Cooperativa de Colombia
  • Diana Forero-Escobar Universidad Cooperativa de Colombia
  • Nerly Fernanda Gutiérrez-Medina Universidad Cooperativa de Colombia
  • Francy Tatiana Yañez-Velandia Universidad Cooperativa de Colombia
  • Carlos Andrés Romero-Rojas Universidad Cooperativa de Colombia



Cast core, Microbiological analysis, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.


Introduction: all dental treatments should strictly follow aseptic protocols in order to reduce failure, especially when performing endodontic procedures. Despite being a key recommendation in this type of interventions, this statement is generally ignored, as students and clinicians tend to neglect the sterilization of posts prior to their use. To raise awareness on this practice, the objective of this study was to demonstrate the presence of microorganisms that cause failure, such as Enterococcus faecalis, Staphylococcus aureus and Candida albicans, in non-sterile cores. Methods: during the first half of 2016, fabricated cast cores were collected in the dental clinics of Universidad Cooperativa de Colombia at Villavicencio. The cores were immersed in saline solution making dilutions to up to 10–4, and finally inserted in duplicate into differential mediums for the microorganisms under study. The candidate colonies were then quantified and selected for the microorganisms under study, performing identification and confirmation in a certified clinical laboratory. Results: the presence of E. faecalis was detected in one of the cores (3.2%) used in the clinic, quantified in 5x104 CFU/ml. The presence of S. aureus or C. albicans was not identified, but other microorganisms were found, such as Candida parapsilopsis (35.5%), Candida tropicalis (6.5%), Kokuria kristinae (16.1%), Staphylococcus saprophyticus (12.9%) and Stenotrophomona maltophilia (3.2%). Conclusion: out of the microorganisms analyzed in this study, only E. faecalis was identified. However, other microorganisms associated with endodontic failure or other type of complications were identified.

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Author Biographies

María del Pilar Angarita-Díaz, Universidad Cooperativa de Colombia

PhD in Biotechnology. Professor of Oral Microbiology, researcher at the GIOMET. School of Dentistry, Universidad Cooperativa de Colombia, Villavicencio. 

Diana Forero-Escobar, Universidad Cooperativa de Colombia

DMD, Magister in Management. Program Head, School of Dentistry. Universidad Cooperativa de Colombia, Villavicencio 

Nerly Fernanda Gutiérrez-Medina, Universidad Cooperativa de Colombia

Ninth semester dental students. School of Dentistry. Universidad Cooperativa de Colombia, Villavicencio. This article is one of the requirements to qualify for the title of DMD 

Francy Tatiana Yañez-Velandia, Universidad Cooperativa de Colombia

Ninth semester dental students. School of Dentistry. Universidad Cooperativa de Colombia, Villavicencio. This article is one of the requirements to qualify for the title of DMD

Carlos Andrés Romero-Rojas, Universidad Cooperativa de Colombia

Ninth semester dental students. School of Dentistry. Universidad Cooperativa de Colombia, Villavicencio. This article is one of the requirements to qualify for the title of DMD


Li X, Kolltveit KM, Tronstad L, Olsen I. Systemic diseases caused by oral infection. Clin Microbiol Rev 2000; 13(4): 547–558.

Shweta, Prakash SK. Dental abscess: a microbiological review. Dent Res J (Isfahan) 2013; 10(5): 585–591.

Carrotte P. Endodontics: part 1. The modern concept of root canal treatment. Br Dent J 2004; 197(4): 181–183. DOI: 10.1038/sj.bdj.4811565 URL:

Mwangi KJ. Knowledge and practice of aseptic techniques among dental students in the University of Nairobi dental hospital [Internet]. Nairobi: University of Nairobi; 2006. Disponible en:

Naik S, Khanagar S, Kumar A, Vadavadagi S, Neelakantappa H, Ramachandra S. Knowledge, attitude, and practice of hand hygiene among dentists practicing in Bangalore city - A cross-sectional survey. J Int Soc Prev Community Dent 2014; 4(3): 159-163. DOI: 10.4103/2231-0762.142013 URL:

Yüzbasioglu E, Saraç D, Canbaz S, Saraç YS, Cengiz S. A survey of cross-infection control procedures: knowledge and attitudes of Turkish dentists. J Appl Oral Sci 2009; 17(6): 565–569.

Helmly SB, Coulton KM, Adams DP. A study of aseptic techniques in a dental hygiene educational clinic. J Dent Hyg 2007; 81(4)

Matsuda JK, Grinbaum RS, Davidowicz H. The assessment of infection control in dental practices in the municipality of São Paulo. Braz J Infect Dis 2011; 15(1): 45–51. DOI: 10.1016/S1413-8670(11)70139-8 URL:

Al-Haroni M, Skaug N. Knowledge of prescribing antimicrobials among Yemeni general dentists. Acta Odontol Scand 2006; 64(5): 274–280. DOI: 10.1080/00016350600672829 URL:

Fuentes R, Weber B, Flores T, Oporto G. Uso de profilaxis antibiótica en implantes dentales : revisión de la literatura. Int J Odontostomat 2010; 4(1): 5–8. DOI: 10.4067/S0718-381X2010000100001 URL:

Barzuna M, Lara D. Descripción del manejo aséptico pre-trans colocación de espiga colada/poste prefabricado. Asociación Costarricense Congresos Odontológicos 2006; 13: 80-86. Disponible en:

Aslam A, Panuganti V, Nanjundasetty JK, Halappa M, Krishna V. Knowledge and attitude of endodontic postgraduate students toward sterilization of endodontic files: a cross-sectional study. Saudi Endod J 2014; 4(1): 18-22. DOI: 10.4103/1658-5984.127982 URL:

Abbott PV, Salgado JC. Strategies for the endodontic management of concurrent endodontic and periodontal diseases. Aust Dent J 2009; 54(Suppl 1): S70–S85. DOI: 10.1111/j.1834-7819.2009.01145.x URL:

Wong AW, Zhang C, Chu CH. A systematic review of nonsurgical single-visit versus multiple-visit endodontic treatment. Clin Cosmet Investig Dent 2014; 6: 45–56. DOI: 10.2147/CCIDE.S61487 URL:

Baumgartner JC, Bakland LK, Sugita EI. Microbiology of endodontics and asepsis in endodontic practice. En: Ingle J, Bakland L (ed). Endodontics. 5 ed. London: BC Decker Inc; 2002. 63-93.

Trope M, Bergenholtz G. Microbiological basis for endodontic treatment: can a maximal outcome be achieved in one visit? Endod Top 2002; 1: 40–53.

Farber PA, Seltzer S. Endodontic Microbiology . I . Etiology. J Endod 1988; 14(7): 363-371. DOI: 10.1016/S0099-2399(88)80200-0 URL:

Peciuliene V, Maneliene R, Balcikonyte E, Drukteinis S, Rutkunas V. Microorganisms in root canal infections: a review. Stomatologija 2008; 10(1): 4-9.

Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 2001; 34(6): 429–434.

Duggan JM, Sedgley CM. Biofilm formation of oral and endodontic enterococcus faecalis. J Endod 2007; 33(7): 815–818. DOI: 10.1016/j.joen.2007.02.016 URL:

Silva-Herzog D, Garcia CC, Rodríguez MH, González AM. Invasión por Candida albicans y Enterococcus faecalis en dentina humana. Rev Nal Odontol Mex 2011; 3(7): 4–8.

Gomes BP, Ferraz CC, Vianna ME, Rosalen PL, Zaia AA, Teixeira FB et al. In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms. Braz Dent J 2002; 13(3): 155–161.

Peciuliene V, Balciuniene I, Eriksen HM, Haapasalo M. Isolation of enterococcus faecalis in previously Root-Filled Canals in a Lithuanian Population. J Endod 2000; 26(10): 593–595. DOI: 10.1097/00004770-200010000-00004 URL:

Zhang C, Du J, Peng Z. Correlation between Enterococcus faecalis and Persistent Intraradicular Infection Compared with Primary Intraradicular Infection: A Systematic Review. J Endod; 2015; 41(8): 1207–1213. DOI: 10.1016/j.joen.2015.04.008 URL:

Stevens RH, Grossman LI. Evaluation of the antimicrobial potential of calcium hydroxide as an intracanal medicament. J Endod 1983; 9(9): 372–374. DOI: 10.1016/S0099-2399(83)80187-3 URL:

Hubble TS, Hatton JF, Nallapareddy SR, Murray BE, Gillespie MJ. Influence of Enterococcus faecalis proteases and the collagen-binding protein, Ace, on adhesion to dentin. Oral Microbiol Immunol 2003; 18(2): 121–126.

Reader CM, Boniface M, Bujanda-Wagner S. Refractory endodontic lesion associated with Staphylococci aureus. J Endod 1994; 20(12): 607–609. DOI: 10.1016/S0099-2399(06)80087-7 URL:

Hegde AM, Pallavi L. Prevalence of selected microorganisms in the pulp space of human deciduous teeth with irreversible pulpitis. Endodontology 2013; 25(1): 107–111.

Gomes C, Fidel S, Fidel R, de-Moura-Sarquis MI. Isolation and taxonomy of filamentous fungi in endodontic infections. J Endod 2010; 36(4): 626–629. DOI: 10.1016/j.joen.2010.01.016 URL:

Siqueira JF Jr, Sen BH. Fungi in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004; 97(5): 632–641. DOI: 10.1016/S1079210404000046 URL:

Pan H, Zhang Y, He GX, Katagori N, Chen H. A comparison of conventional methods for the quantification of bacterial cells after exposure to metal oxide nanoparticles. BMC Microbiol 2014; 14(1): 222. Disponible en: DOI: 10.1186/s12866-014-0222-6 URL:

Samra Z, Heifetz M, Talmor J, Bain E, Bahar J. Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens evaluation of use of a new chromogenic agar in detection of urinary tract pathogens. J Clin Microbiol 1998; 36(4): 990–994. DOI: URL:

Kroning IS, Iglesias MA, Sehn CP, Valente Gandra TK, Mata MM, da Silva WP. Staphylococcus aureus isolated from handmade sweets: biofilm formation, enterotoxigenicity and antimicrobial resistance. Food Microbiol 2016; 58: 105–111. DOI: 10.1016/ URL:

Odds FC, Bernaerts R. CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol 1994; 32(8): 1923–1929.

Romeu B, Salazar P, Navarro A, Lugo D, Hernández U; Rojas N et al. Utilidad del sistema VITEK en la identificación y determinación de la susceptibilidad antimicrobiana de bacterias aisladas de ecosistemas dulceacuícolas. Rev CENIC Ciencias Biol 2010; 41: 1–9.

Lana A, Ribeiro-Sobrinho AP, Stehling R, Garcia GD, Silva BK, Hamdan JS et al. Microorganisms isolated from root canals presenting necrotic pulp and their drug susceptibility in vitro. Oral Microbiol Immunol 2001; 16(2): 100–105.

Pinheiro ET, Gomes BP, Ferraz CC, Teixeira FB, Zaia AA, Souza-Filho FJ. Evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility. Oral Microbiol Immunol 2003; 18(2): 100–103.

Ingle JI, Bakland LK, Baumgartner JC. Ingle’s endodontics 6. 6 ed. Bindner P, editor. Hamilton, Ontario: BC Decker; 2008.

Ensinas P, Zacca R, Iriarte M. Estudio microbiológico de pernos colados antes de ser cementados en el conducto radicular. Canal Abierto 2006; 14-17.

Kristich CJ, Rice LB, Arias CA. Enterococcal infection: treatment and antibiotic resistance. En: Gilmore MS. Enterococci: from commensals to leading causes of drug resistant infection. Boston: Massachusetts Eye and Ear Infirmary; 2014.

Melo-Maltos SM. Influência da dose infectante na colonização bacteriana do sistema de canais radiculares e na translocação para linfonodos submandibulares em camundongos gnotobióticos. [Trabajo de grado maestría en Endodoncia]. Belo Horizonte: Universidade Federal de Minas Gerais. Faculdade de Odontologia; 2001.

Friedman S. Prognosis in the treatment of teeth with endodontic infections. En: Fouad AF. Endodontic microbiology. 1 ed. Singapore: Wiley-Blackwell; 2009. 281-319.

Hibbing ME, Fuqua C, Parsek MR, Peterson SB. Bacterial competition: surviving and thriving in the microbial jungle. Nat Rev Microbiol 2010; 8(1): 15–25. DOI: 10.1038/nrmicro2259 URL:

Park B, Nizet V, Liu GY. Role of Staphylococcus aureus catalase in niche competition against Streptococcus pneumoniae. J Bacteriol 2008; 190(7): 2275–2278. DOI: 10.1128/JB.00006-08 URL:

Slack G. The resistance to antibiotics of microorganisms isolated from root canals. Br Dent 1957; 18: 493–494.

Kothavade RJ, Kura MM, Valand AG, Panthaki MH. Candida tropicalis: Its prevalence, pathogenicity and increasing resistance to fluconazole. J Med Microbiol 2010; 59(8): 873–880. DOI: 10.1099/jmm.0.013227-0 URL:

Fernandes T, Silva S, Henriques M. Candida tropicalis biofilm’s matrix-involvement on its resistance to amphotericin B. Diagn Microbiol Infect Dis 2015; 83(2): 165–169. DOI: 10.1016/j.diagmicrobio.2015.06.015 URL:

Fernández-Ruiz M, Puig-Asensio M, Guinea J, Almirante B, Padilla B, Almela M et al. Candida tropicalis bloodstream infection: incidence, risk factors and outcome in a population-based surveillance. J Infect 2015; 71(3): 385–394. DOI: 10.1016/j.jinf.2015.05.009 URL:

Silva S, Negri M, Henriques M, Oliveira R, Williams DW, Azeredo J. Candida glabrata, Candida parapsilosis and Candida tropicalis: Biology, epidemiology, pathogenicity and antifungal resistance. FEMS Microbiol Rev 2012; 36(2): 288–305. DOI: 10.1111/j.1574-6976.2011.00278.x URL:

Pfaller MA, Diekema DJ. Epidemiology of invasive candidiasis: A persistent public health problem. Clin Microbiol Rev 2007; 20(1): 133–163. DOI: 10.1128/CMR.00029-06 URL:

Rossignol T, Ding C, Guida A, d’Enfert C, Higgins DG, Butler G. Correlation between biofilm formation and the hypoxic response in Candida parapsilosis. Eukaryot Cell 2009; 8(4): 550–559. DOI: 10.1128/EC.00350-08 URL:

Singh R, Parija SC. Candida parapsilosis: an emerging fungal pathogen. Indian J Med Res 2012; 136(4): 671–673.

George M, Ivancaková R. Root canal microflora. Acta Medica (Hradec Králové) 2007; 50(1): 7–15.

Kutllovci TA, Iljovska S, Begzati A, Jankulovska M, Popovska M, Rexhepi A et al. Bacteriological identification of selected pathogens in infected primary and young permanent teeth associated with clinical symptoms. Open J Med Microbiol 2015; 5(2): 59–68. DOI: 10.4236/ojmm.2015.52007 URL:

Garcia DDO, Timenetsky J, Martinez MB, Francisco W, Sinto SI, Yanaguita RM. Proteases (caseinase and elastase), hemolysins, adhesion and susceptibility to antimicrobials of Stenotrophomonas maltophilia isolates obtained from clinical specimens. Braz J Microbiol 2002; 33(2): 157–162. DOI: 10.1590/S1517-83822002000200012 URL:

Choi SH, Woo JH, Jeong JY, Kim NJ, Kim MN, Kim YS et al. Clinical significance of Staphylococcus saprophyticus identified on blood culture in a tertiary care hospital. Diagn Microbiol Infect Dis 2006; 56(3): 337–339. DOI: 10.1016/j.diagmicrobio.2006.08.012 URL:




How to Cite

Angarita-Díaz, M. del P., Forero-Escobar, D., Gutiérrez-Medina, N. F., Yañez-Velandia, F. T., & Romero-Rojas, C. A. (2017). Analysis of Enterococcus Faecalis, Staphylococcus Aureus, and Candida Albicans in cast metal cores. Revista Facultad De Odontología Universidad De Antioquia, 28(2), 292–310.