Use of laser fluorescence in the diagnosis of enamel caries: systemic review and meta-analysis
DOI:
https://doi.org/10.17533/udea.rfo.v38n1e362064Keywords:
dental caries, fluorescence, sensitivity and specificity, meta-analysisAbstract
Introduction: caries is one of the most prevalent diseases worldwide. Traditional diagnostic methods show significant limitations in sensitivity and specificity. Laser fluorescence has emerged as a complementary tool that provides more reliable and reproducible measurements. This study evaluated the diagnostic accuracy of laser fluorescence for the identification of enamel caries through a meta-analysis of sensitivity, specificity, and area under the ROC curve. Methods: a systematic search was conducted in PubMed and LILACS databases (2019 - 2024), to identify original studies that met the eligibility criteria defined by PIRDS methodological guidelines for diagnostic tests. Clinical and in vitro studies assessing the diagnostic accuracy of laser fluorescence for enamel caries were included. Studies on dentin, root lesions, and artificially induced caries were excluded. The main study variables were extracted and described. Results: from an initial sample of 84 articles, seven met the eligibility criteria. The wavelength used in most studies was 655 nm. Sensitivity had a mean of 0.7988 (95 % CI 0.7345 – 0.8632). Specificity had a mean of 0.7567 (95 % CI 0.5539 – 0.9595). The area under the ROC curve had a mean of 0.800 (95 % CI 0.7085 – 0.8915). These findings indicate that laser fluorescence has acceptable diagnostic performance, although its accuracy varies depending on clinical context and methodology used. Conclusion: laser fluorescence is a useful complementary tool for the diagnosis of enamel caries, standing out for its validity and reliability. However, it should not be used as a stand-alone diagnostic method.
Downloads
References
Cubero Santos A, Lorido Cano I, González Huéscar A, Ferrer García MÁ, Zapata Carrasco MD, Ambel Sánchez JL. Prevalencia de caries dental en escolares de educación infantil de una zona de salud con nivel socioeconómico bajo. Rev Pediatr Aten Primaria. 2019; 21(82): e47-59.
Morales Miranda L, Gómez González W. Caries dental y sus consecuencias clínicas relacionadas al impacto en la calidad de vida de preescolares de una escuela estatal. Rev Estomatol Herediana. 2019; 29(1): 17-29. DOI: https://doi.org/10.20453/reh.v29i1.3491
AbdELkader AR, Hafez Ibrahim S, Elsayed Hassanein O. Reliability of impedance spectroscopy versus digital radiograph and ICDAS-II in occlusal caries detection: a prospective clinical trial. Sci Rep. 2024; 1(14): 16553. DOI: https://doi.org/10.1038/s41598-024-66627-4
NouhzadehMalekshah S, Fekrazad R, Bargrizan M, Kalhori KA. Evaluation of laser fluorescence in combination with photosensitizers for detection of demineralized lesions. Photodiagnosis PhotodynTher. 2019; 26: 300-5. DOI: https://doi.org/10.1016/j.pdpdt.2019.03.019
Laverde-Albarracín DM. La laserfluorescencia cualitativa en el diagnóstico del límite cavitario. Rev Inf Cient. 2023; 102: 4370. DOI: https://doi.org/10.5281/zenodo.10136113
Monea M, Eşian D, Vlad RE, Bica CI. In vivo effectiveness of visual inspection and laser fluorescence in the diagnosis of early pit-and-fissure carious lesions: a cross-sectional study in a group of Romanian children. Medicine (Baltimore). 2021; 100(45): e27811. DOI: https://doi.org/10.1097/md.0000000000027811
Marcondes APM, de Campos PH, Ribeiro CS, de Novaes TF de, Lussi A, Diniz MB. Performance of near-infrared light transillumination in the detection of occlusal caries lesions in deciduous teeth. Photodiagnosis Photodyn Ther. 2023; 44: 103744. DOI: https://doi.org/10.1016/j.pdpdt.2023.103744
Sardana D, Li KY, Ekambaram M, Yang Y, McGrath CP, Yiu CK. Validation of clinical photography and a laser fluorescence device for assessment of enamel demineralization during multi-bracketed fixed orthodontic treatment. Photodiagnosis Photodyn Ther. 2022; 38: 102828. DOI: https://doi.org/10.1016/j.pdpdt.2022.102828
Litzenburger F, Schäfer G, Hickel R, Kühnisch J, Heck K. Comparison of novel and established caries diagnostic methods: a clinical study on occlusal surfaces. BMC Oral Health. 2021; 21(1): 97. DOI: https://doi.org/10.1186/s12903-021-01465-8
Terrer E, Slimani A, Giraudeau N, Levallois B, Tramini P, Bonte E, et al. Performance of fluorescence-based systems in early caries detection: a public health issue. J Contemp Dent Pract. 2019; 10(20): 1126-31.
Park KJ, Voigt A, Schneider H, Ziebolz D, Haak R. Light-based diagnostic methods for the in vivo assessment of initial caries lesions: laser fluorescence, QLF and OCT. Photodiagnosis Photodyn Ther. 2021; 34: 102270. DOI: https://doi.org/10.1016/j.pdpdt.2021.102270
Nair MJ, Rao A, Jayaprakash K, Natarajan S, Kumblekar V, Suprabha BS. Effectiveness of laser fluorescence–based device in detecting the extent of re-mineralisation in primary teeth compared to the conventional method: an in vitro: study. Indian J Dent Res. 2022; 33(3): 292-6. DOI: https://doi.org/10.4103/ijdr.ijdr_546_21
Campbell JM, Klugar M, Ding S, Carmody DP, Hakonsen SJ, Jadotte YT, et al. Diagnostic test accuracy: methods for systematic review and meta-analysis. Int J Evid Based Healthc. 2015; 13(3): 154-62. DOI: https://doi.org/10.1097/xeb.0000000000000061
Kreher D, Park KJ, Schmalz G, Schulz-Kornas E, Haak R, Ziebolz D. Evaluation of quantitative light-induced fluorescence to assess lesion depth in cavitated and non-cavitated root caries lesions: an in vitro study. Photodiagnosis Photodyn Ther. 2022; 37: 102675. DOI: https://doi.org/10.1016/j.pdpdt.2021.102675
Vizcaíno-Salazar GJ. Importancia del cálculo de la sensibilidad, la especificidad y otros parámetros estadísticos en el uso de las pruebas de diagnóstico clínico y de laboratorio. Med Lab. 2017; 23(7-8): 365-86.
Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. Declaración PRISMA 2020: una guía actualizada para la publicación de revisiones sistemáticas. Rev Esp Cardiol. 2021; 74(9): 790-9. DOI: https://doi.org/10.1016/j.rec.2021.07.010
El-Sharkawy YH, Elbasuney S. Non-invasive caries detection and delineation via novel laser-induced fluorescence with hyperspectral imaging. Spectrochim Acta A Mol Biomol Spectrosc. 2020; 240: 118616. DOI: https://doi.org/10.1016/j.saa.2020.118616
Ro JH, Bang JJ, Kim YI, Lee DJ, Ko CC, Garcia-Godoy F, et al. Spectral characteristics of caries autofluorescence obtained from different locations and caries severities. J Biophotonics. 2020; 13(1): e201900224. DOI: https://doi.org/10.1002/jbio.201900224
Gómez Clavel JF, Vences DT, Valdiviezo AC, Peña Castillo E, García Muñoz A, Trejo Iriarte CG. Correlation between caries history and laser fluorescence of sound tooth enamel in restored and unrestored dentition. J Oral Sci. 2019; 61(3): 441-6. DOI: https://doi.org/10.2334/josnusd.18-0183
Tassoker M, Ozcan S, Karabekiroglu S. Occlusal caries detection and diagnosis using visual ICDAS Criteria, laser fluorescence measurements, and near-infrared light transillumination images. Med Princ Pract. 2020; 29(1): 25-31. DOI: https://doi.org/10.1159/000501257
Gondim Silva R, Abreu LG. Estudo da acurácia de dispositivos de indução da fluorescência e do método radiográfico para o diagnóstico de lesões de cárie em dentes decíduos: uma revisão sistemática e meta-análise. Arq odontol. 2022; 58: 63-86. DOI: https://doi.org/10.35699/2178-1990.2022.35908
Kapor S, Rankovic MJ, Khazaei Y, Crispin A, Schüler I, Krause F, et al. Systematic review and meta-analysis of diagnostic methods for occlusal surface caries. Clin Oral Investig. 2021; 8(25): 4801-15. DOI: https://doi.org/10.1007/s00784-021-04024-1
Downloads
Published
How to Cite
Issue
Section
Categories
License
Copyright (c) 2025 Revista Facultad de Odontología Universidad de Antioquia

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright Notice
Copyright comprises moral and patrimonial rights.
1. Moral rights: are born at the moment of the creation of the work, without the need to register it. They belong to the author in a personal and unrelinquishable manner; also, they are imprescriptible, unalienable and non negotiable. Moral rights are the right to paternity of the work, the right to integrity of the work, the right to maintain the work unedited or to publish it under a pseudonym or anonymously, the right to modify the work, the right to repent and, the right to be mentioned, in accordance with the definitions established in article 40 of Intellectual property bylaws of the Universidad (RECTORAL RESOLUTION 21231 of 2005).
2. Patrimonial rights: they consist of the capacity of financially dispose and benefit from the work trough any mean. Also, the patrimonial rights are relinquishable, attachable, prescriptive, temporary and transmissible, and they are caused with the publication or divulgation of the work. To the effect of publication of articles in the journal Revista de la Facultad de Odontología, it is understood that Universidad de Antioquia is the owner of the patrimonial rights of the contents of the publication.
The content of the publications is the exclusive responsibility of the authors. Neither the printing press, nor the editors, nor the Editorial Board will be responsible for the use of the information contained in the articles.
I, we, the author(s), and through me (us), the Entity for which I, am (are) working, hereby transfer in a total and definitive manner and without any limitation, to the Revista Facultad de Odontología Universidad de Antioquia, the patrimonial rights corresponding to the article presented for physical and digital publication. I also declare that neither this article, nor part of it has been published in another journal.
Open Access Policy
The articles published in our Journal are fully open access, as we consider that providing the public with free access to research contributes to a greater global exchange of knowledge.
Creative Commons License
The Journal offers its content to third parties without any kind of economic compensation or embargo on the articles. Articles are published under the terms of a Creative Commons license, known as Attribution – NonCommercial – Share Alike (BY-NC-SA), which permits use, distribution and reproduction in any medium, provided that the original work is properly cited and that the new productions are licensed under the same conditions.
![]()
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.



