Indispensable amino acid deprivation does not cause rapid amino acid depletion in fish body and this principle has a potential to be used as the new strategy in nutrition


  • Konrad Dabrowski
  • Yongfang Zhang
  • Murat Arslan
  • Bendik Fyhn Terjesen



Degradation of body proteins occurs not only during fasting but it is a continuous process of renewal of proteins, including those degraded proteosomally immediately after synthesis which apparently accounts for 30% of all newly formed (18), as well as those “storage” proteins that are hydrolyzed at much slower rate by cytoplasmic proteases. If this concept is applied to acute deficiency in indispensable amino acids in food, then such imbalances may in fact become imperfections physiologically overridden by continuous flow of amino acids returned to synthesis sites.
Studies conducted in animals have shown that an amino acid imbalanced diet reduced feed intake (10, 16) and growth of animals (5, 8). . In addition, studies have also demonstrated that rats could recover from such deficiency when fed with feed containing the specific IDAA they were lacking (12). . Recently, Hao et al, (2005) argued that mammals recognize dietary IDAA deficiency in the brain‘s anterior cortex that is signaling diet rejection (7). . However, there was no information available on how IDAA deficient diets affect fish feed intake, feed utilization and differences in feed acceptance in a short term (within hours) and during a long period (within several weeks) until recently. We presented evidence that fish may in fact differ from a mammalian model, and a generalization over the anorectic response in the case of lacking IDAA did not apply (3).

Amino acid balanced diets in monogastric animals must be composed of various protein sources to provide the right proportion of all IDAA. Compared to fish meal, the major feed protein sources, such as plant proteins and meat and bone meal are deficient in methionine, whereas oilseeds are deficient in lysine. Therefore, these ingredients may not be used as the sole protein source in formulated, high-protein content (45-55%) diets. Under practical farming conditions, feed formulations that include plant proteins may result in disproportionate amounts of IDAA and IDAA deficiencies. IDAA deficient diets affected animals adversely, including depression of food intake and growth, change of feed intake behavior, development of lesions and result in low survival rates (8). Previous studies showed that feed intake of rats decreased significantly after they were fed a threonine deficient diet in comparison to control group (10, 11). The level of plasma histidine and threonine decreased rapidly after ingesting histidine or threonine deficient diets respectively, while the level of plasma total IDAA other than histidine and threonine increased significantly. There was no significant change in the plasma dispensable amino acid (DAA) concentrations after rats consumed histidine or threonine deficient diets. Liver and muscle histidine or threonine concentrations decreased after rats were fed histidine or threonine imbalanced diets (14). Feurte et al, (1999) also reported that plasma threonine concentrations significantly decreased between 30 to 60 minutes after rats ingested a threonine devoid diet (4).

The mechanism of interdependence of behavioral response (food rejection) and physiological indicators (concentration of IDAA in tissue) is being addressed systematically in mammals. . Studies showed that a threonine-imbalanced diet significantly decreased threonine concentrations in the anterior piriform cortex (APC) of the brain (5). . This correlated with rats rapidly rejecting IDAA deficient diet i.e. within 15 min after ingestion of threonine-imbalanced diet. Recent studies by Koehnle et al (2003) confirmed that rats recognized threonine deficient diet within the first meal and reduced the first meal duration and rejected the threonine deficient diet within 12-16 min (9). . It has been long recognized, however, that animals as ancient as spiders (6) optimize proportions of IDAA in their diets by adapting specific strategy of nutritional polyphagy. By diversification of prey (protein and amino acid composition), predatory insects arrive at selecting nutrients not only by quantity but also most importantly by quality (13).

Weekes et al (2006) suggested an alternative method of induced amino acid imbalance in mammals by abomasal infusion of free amino acid solution lacking one or more indispensable amino acids (20). This is the first attempt to our knowledge that defined response in blood amino acid following prolonged deficiency in healthy animal. However, using fish model may prove to provide enormous advantage in respect to opportunity to follow the dietary impact on the “whole animal level”, whole body free amino acid concentrations.

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. Barluenga M, Meyer A . The midas cichlid species complex: incipient sympatric speciation in Nicaraguan cichlid fishes? Mol Ecology 2004; 13:2061-2076.

. Dabrowski K, Arslan M, Terjesen BF, Zhang Y . The effect of dietary indispensable amino acid imbalances on feed intake: Is there sensing of deficiency and neural signaling present in fish? Aquaculture 2007; 268:136-142.

. Dabrowski K, Terjesen BF, Zhang Y, Phang JM, Lee KJ . A concept of dietary dipeptides: a step to resolve the problem of amino acid availability in early life of vertebrates . J Exp Biol 2005; 208:2885-2894 .

. Feurte S, Nicolaidis S, Even PC, Tome D, Mahe S, et alRapid fall in plasma threonine followed by increased intermeal interval in response to first ingestion of a threonine-devoid diet in rats . Appetite 1999; 33:329-341 .

. Gietzen DW . Neural mechanisms in the responses to amino acid deficiency. J Nutr 1993; 123:610-625.

. Greenstone MH . Spider feeding behavior optimizes dietary essential amino acid composition . Nature 1979; 282:501-503 .

. Hao S, Sharp JW, Ross-Inta CM, McDaniel BJ, Anthony TG, et al Uncharged tRNA and sensing of amino acid deficiency in mammalian piriform cortex. Science 2005; 307:1776-1778 .

. Harper AE, Benevenga NJ, Wohlhueter RM . Effects of ingestion of disproportionate amounts of amino acids . Psychol Rev 1970; 50:428-558 .

. Koehnle TJ, Russell MC; Gietzen DW . Rats rapidly reject diets deficient in essential amino acids. J Nutr 2003; 133:2331-2335 .

. Koehnle TJ, Russell MC, Morin AS, Erecius LF, Gietzen DW . Diets deficient in indispensable amino acids rapidly decrease the concentration of the limiting amino acid in the anterior piriform cortex of rats . J Nutr 2004; 134:2365-2371 .a

. Koehnle TJ, Stephens AL, Gietzen DW . Threonine imbalanced diet alters first meal-microstructure in rats. Physiol Behav 2004; 81:15-21 .b

. Markison S, Thompson BL, Smith JC, Spector AC . Time course and pattern of compensatory ingestive behavioral adjustments to lysine deficiency in rats. J Nutr 2000; 130:1320-1328 .

. Mayntz DR, Raubenheimer D, Salomon M, Toft S, Simpson SJ. Nutrient-specific foraging in invertebrate predators . Science 2005; 307:111-113 .

. Peng Y, Tews JK, Harper AE . Amino acid imbalance, protein intake, and changes in rat brain amino acids . Am J Physiol 1972; 222:314-321 .

. Rogers QR, Wigle AR, Laufer A, Castellanos VH, Morris JG . Cats select for adequate methionine but not threonine . J Nutr 2004; 134(8 Suppl): 2046S-2049S .

. Russell MC, Koehnle TJ, Barrett JA . The rapid anorectic response to a threonine imbalanced diet is decreased by injection of threonine into the anterior piriform cortex of rats . Nutr Neurosci 2003; 6:247-251 .

. Santiago CB, Lovell RT . Amino acid requirements for growth of Nile tilapia . J Nutr 1988; 118:1540-1546 .

. Schubert U, Anton LC, Gibbs J, Norbury CC, Yewdell JW, et al Rapid degradation of a large fraction of newly synthesized proteins by proteasomes . Nature 2000; 404:770-774 .

. Terjesen BF, Park K, Tesser MB, Portella MC, Zhang Y, et al Lipoic acid and ascorbic acid affect plasma freeLipoic acid and ascorbic acid affect plasma free amino acids selectively in the teleost fish pacu (Piaractus mesopotamicus) . J Nutr 2004; 134:2930-2934 .2004; 134:2930-2934 .134:2930-2934 .

. Weekes TL, Luimes PH, Cant JP . Responses to amino acid imbalances and deficiencies in lactating dairy cows. J Dairy Sci 2006; 89:2177-2187 .




How to Cite

Dabrowski, K., Zhang, Y., Arslan, M., & Fyhn Terjesen, B. (2016). Indispensable amino acid deprivation does not cause rapid amino acid depletion in fish body and this principle has a potential to be used as the new strategy in nutrition. Revista Colombiana De Ciencias Pecuarias, 20(4), 4.




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